A transcriptomic analysis on gene expressions in the infective third and pathogenic fifth larval stages of Angiostrongylus cantonensis

Chang, Shwu Fen; Tang, Petrus; Yen, Chuan-Min; Chow, Kai-Ping N.; Wang, Lian-Chen

doi:10.1016/j.parint.2013.08.012

Cited by 8 publications

(5 citation statements)

References 45 publications

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“…While some genes are expressed constitutively across all stages, some are stage specific. Chang et al (2014 a ) found that transcripts exclusive to L 5 worms were related to growth, development, sexual differentiation and reproduction, while L3-specific transcripts were mostly related to metabolism. The classes of proteases expressed between these two stages also differed (Chang et al 2014 a ).…”

Section: Molecular Biologymentioning

confidence: 99%

“…Chang et al (2014 a ) found that transcripts exclusive to L 5 worms were related to growth, development, sexual differentiation and reproduction, while L3-specific transcripts were mostly related to metabolism. The classes of proteases expressed between these two stages also differed (Chang et al 2014 a ). Huang et al (2013) observed differences in galectin abundance in the soluble proteomes of male L 5 & male adult worms, and female adult & male adult worms.…”

Section: Molecular Biologymentioning

confidence: 99%

“…Proteases are important virulence factors in other pathogenic helminths, essential for tissue penetration and nutrient acquisition (Stack et al 2011; McVeigh et al 2012; Morassutti and Graeff-Teixeira, 2012). Chang et al (2014 a ) identified transcripts for proteases of various types in the L 3 and L 5 stages; most notably metalloproteases, aspartic proteases & cysteine proteases in the L 3 stage and cysteine, aspartic & serine proteases in the L 5 stage. Serine proteases of A. cantonensis react to sera from infected patients (Morassutti et al 2012 b ), and a recombinant asparaginyl endopeptidase was recognized by serum from infected BALB/c and ICR strain mice and, by serum from human angiostrongyliasis patients (Chang et al 2014 b ).…”

Section: Molecular Biologymentioning

confidence: 99%

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Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen

et al. 2016

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Angiostrongylus cantonensis is a metastrongyloid nematode found widely in the Asia-Pacific region, and the aetiological agent of angiostrongyliasis; a disease characterized by eosinophilic meningitis. Rattus rats are definitive hosts of A. cantonensis, while intermediate hosts include terrestrial and aquatic molluscs. Humans are dead-end hosts that usually become infected upon ingestion of infected molluscs. A presumptive diagnosis is often made based on clinical features, a history of mollusc consumption, eosinophilic pleocytosis in cerebral spinal fluid, and advanced imaging such as computed tomography. Serological tests are available for angiostrongyliasis, though many tests are still under development. While there is no treatment consensus, therapy often includes a combination of anthelmintics and corticosteroids. Angiostrongyliasis is relatively rare, but is often associated with morbidity and sometimes mortality. Recent reports suggest the parasites' range is increasing, leading to fatalities in regions previously considered Angiostrongylus-free, and sometimes, delayed diagnosis in newly invaded regions. Increased awareness of angiostrongyliasis would facilitate rapid diagnosis and improved clinical outcomes. This paper summarizes knowledge on the parasites' life cycle, clinical aspects and epidemiology. The molecular biology of Angiostrongylus spp. is also discussed. Attention is paid to the significance of angiostrongyliasis in Australia, given the recent severe cases reported from the Sydney region.

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Section: Molecular Biologymentioning

confidence: 99%

Section: Molecular Biologymentioning

confidence: 99%

Section: Molecular Biologymentioning

confidence: 99%

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Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen

et al. 2016

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“…Different parasitic nematode species and different developmental stages in a certain nematode species may have different repertoire of proteases for adapting themselves to a specific niche, and comparative analyses of these differences would be essential to understand their pathogenesis and develop potential control strategies. For example, transcriptome analysis of a parasitic nematode Angiostrongylus cantonensis revealed that the infective third stage larvae highly expressed metallo-, aspartic-, and cysteine protease, while the fifth stage larvae highly expressed cysteine-, aspartic-, and serine proteases ( Chang et al, 2014 ), suggesting these proteases have different roles in different developmental stages of parasites. Moreover, transcript profiles of aspartic protease genes (e.g., Sc-ASP110, 113, 155) in an entomopathogenic nematode Steinernema carpocapsae were characterized and different expression profiles of each transcript were found in the different developmental stages ( Balasubramanian, Nascimento, Ferreira, Martinez, and Simões, 2012 ; Balasubramanian, Toubarro, Nascimento, Ferreira, and Simões, 2012 ; Balasubramanian and Simões, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome sequencing and analysis of Anisakis pegreffii (Nematoda: Anisakidae) third-stage and fourth stage larvae

Nam

Kim

2020

Journal of Nematology

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Anisakis pegreffii is known as one of the causes of a fish-borne zoonosis, anisakidosis. Despite its significant public health and food hygiene impacts, little is known of the pathogenesis, genetic background of this parasite, at least partly due to the lack of genome and transcriptome information. In this study, RNA-seq and de novo assembly were conducted to obtain transcriptome profiles of the A. pegreffii third and fourth larvae. The third stage larvae (APL3) were collected from chub mackerel and the fourth stage larvae (APL4) were obtained by in vitro culture. In total, 47,243 and 43,660 unigenes were expressed in APL3 and APL4 transcriptomes. Of them, 18,753 were known and 28,490 were novel for APL3, while 18,996 were known and 24,664 were novel for APL4. The most abundantly expressed genes in APL3 were mitochondrial enzymes (COI, COII, COIII) and polyubiquitins (UBB, UBIQP_XENLA). Collagen-related genes (col-145, col-34, col-138, Bm1_54705, col-40) were the most abundantly expressed in APL4. Mitochondrial enzyme genes (COIII, COI) were also highly expressed in APL4. Among the transcripts, 614 were up-regulated in APL3, while 1,309 were up-regulated in APL4. Several protease and protein biosynthesis-related genes were highly expressed in APL3, all of which are thought to be crucial for invading host tissues. Collagen synthesis-related genes were highly expressed in APL4, reflecting active biosynthesis of collagens occurs during moulting process of APL4. Of these differentially expressed genes, several genes (SI, nas-13, EF-TSMT, SFXN2, dhs-27) were validated to highly transcribed in APL3, while other genes (col-40, F09E10.7, pept-1, col-34, VIT) in APL4. The biological roles of these genes in vivo will be deciphered when the reference genome sequences are available, together with in vitro experiments.

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“…Diagnosis is sought via clinical criteria, including the presence of cerebrospinal fluid eosinophils and a history of exposure to A. cantonensis larvae (third-larvae, L3), e.g., from raw freshwater snails (Pila or Pomacea snails) or contaminated vegetables [12,13]. Conversely, the diagnosis based on clinical presentation is difficult because the symptoms are often confused with those produced by other parasitic diseases such as paragonimiasis, gnathostomiasis, and cysticercosis [14].…”

Section: Introductionmentioning

confidence: 99%

Angiostrongylus cantonensis an Atypical Presenilin: Epitope Mapping, Characterization, and Development of an ELISA Peptide Assay for Specific Diagnostic of Angiostrongyliasis

et al. 2022

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Background: Angiostrongyliasis, the leading cause universal of eosinophilic meningitis, is an emergent disease due to Angiostrongylus cantonensis (rat lungworm) larvae, transmitted accidentally to humans. The diagnosis of human angiostrongyliasis is based on epidemiologic characteristics, clinical symptoms, medical history, and laboratory findings, particularly hypereosinophilia in blood and cerebrospinal fluid. Thus, the diagnosis is difficult and often confused with those produced by other parasitic diseases. Therefore, the development of a fast and specific diagnostic test for angiostrongyliasis is a challenge mainly due to the lack of specificity of the described tests, and therefore, the characterization of a new target is required. Material and Methods: Using bioinformatics tools, the putative presenilin (PS) protein C7BVX5-1 was characterized structurally and phylogenetically. A peptide microarray approach was employed to identify single and specific epitopes, and tetrameric epitope peptides were synthesized to evaluate their performance in an ELISA-peptide assay. Results: The data showed that the A. cantonensis PS protein presents nine transmembrane domains, the catalytic aspartyl domain [(XD (aa 241) and GLGD (aa 332–335)], between TM6 and TM7 and the absence of the PALP and other characteristics domains of the class A22 and homologous presenilin (PSH). These individualities make it an atypical sub-branch of the PS family, located in a separate subgroup along with the enzyme Haemogonchus contournus and separated from other worm subclasses. Twelve B-linear epitopes were identified by microarray of peptides and validated by ELISA using infected rat sera. In addition, their diagnostic performance was demonstrated by an ELISA-MAP4 peptide. Conclusions: Our data show that the putative AgPS is an atypical multi-pass transmembrane protein and indicate that the protein is an excellent immunological target with two (PsAg3 and PsAg9) A. costarisencis cross-reactive epitopes and eight (PsAg1, PsAg2, PsAg6, PsAg7, PsAg8, PsAg10, PsAg11, PsAg12) apparent unique A. cantonensis epitopes. These epitopes could be used in engineered receptacle proteins to develop a specific immunological diagnostic assay for angiostrongyliasis caused by A. cantonensis.

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A transcriptomic analysis on gene expressions in the infective third and pathogenic fifth larval stages of Angiostrongylus cantonensis

Cited by 8 publications

References 45 publications

Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen

Angiostrongylus cantonensis: a review of its distribution, molecular biology and clinical significance as a human pathogen

De novo transcriptome sequencing and analysis of Anisakis pegreffii (Nematoda: Anisakidae) third-stage and fourth stage larvae

Angiostrongylus cantonensis an Atypical Presenilin: Epitope Mapping, Characterization, and Development of an ELISA Peptide Assay for Specific Diagnostic of Angiostrongyliasis

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