A Triantennary Dendritic Galactoside‐Capped Nanohybrid with a ZnS/CdSe Nanoparticle Core as a Hydrophilic, Fluorescent, Multivalent Probe for Metastatic Lung Cancer Cells

Abstract

A new tri‐antennary, galactoside‐capped gallamide dendritic anchor with thiolate focal group is synthesized via click chemistry. The highly hydrophilic dendron was efficiently anchored onto the surface of CdSe/ZnS core/shell nanoparticles in a covalent fashion, as evidenced by 1H NMR spectrum of the resultant nanohybrid with intact core fluorescent property. The water‐soluble nano‐hybrid was found smoothly and selectively uptaken by lung cancer cells enriched with membrane‐bound asialoprotein receptors in 2–3 h, as evidenced by confocal microscopic analysis. Furthermore, cancer cells that are undergoing active mitosis also tend to uptake the nanohybrids efficiently and remain sustainable in serum‐containing medium for several days, which shed light on its application as a photodynamic drug carrier for apoptosis study.

Methods for the Functionalisation of Nanoparticles: New Insights and Perspectives

“…Another example is the reaction of poly(alkylcyanoacrylate) nanoparticles, made from modified cyanoacrylate polymers, with alkyne substrates like alkyne-dansyl or alkyne-PEG. [54] A similar methodology can be applied to hybrid organic/inorganic particles [55][56][57][58][59][60][61][62][63] for the introduction of biolinkers (biotin), fluorescent markers (indocyanin, fluorescein), steroid moieties (oestrogen) or anti-cancer drugs (paclitaxel). Alkyne or azide groups can easily be introduced into these kinds of molecules.…”

Glyconanomaterials for biosensing applications

“…Covalent approaches include in situ protocols, for example, based on the addition of thiol-functionalized neoglycoconjugates to QD precursor solutions (De la Fuente and Penadés, 2005; Mukhopadhyay et al, 2009). The ligand exchange protocol is similar to the synthesis of gold glyconanomaterials, where the original capping agents can be replaced by thiol-derivatized glycoconjugates (Babu et al, 2007; Chen et al, 2008; Niikura et al, 2007, 2008; Robinson et al., 2005). QD glyconanomaterials have also been prepared by post-modification protocols similar to gold nanoparticles (Higuchi et al, 2008; Kikkeri et al, 2009b; Sandros et al, 2007).…”

Protein–quantum dot nanohybrids for bioanalytical applications

“…Protein–QD hybrids can be prepared by the direct synthesis of QDs in an aqueous solution containing proteins (Figure ). This approach requires fulfillment of the following criteria: (1) the QDs must contain metal ions available for coordination with specific ligands of amino acids, (2) synthetic conditions must avoid harsh conditions that can denature proteins, and (3) the as‐prepared protein‐conjugated QDs must retain their optical properties . Recently, a simple one‐step method was applied for the synthesis of water‐soluble chalcogenide QDs (e.g., CdSe and CdS) coupled with bovine serum albumin (BSA) .…”