A two-component signal-transduction cascade in Carnobacterium piscicola LV17B: two signaling peptides and one sensor-transmitter

Kleerebezem, Michiel; Kuipers, Oscar P.; Vos, Willem M. de; Stiles, Michael E.; Quadri, Luis E. N.

doi:10.1016/s0196-9781(01)00494-6

Cited by 32 publications

(17 citation statements)

References 32 publications

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“…Each repeat consists of 9 bp separated by an AT-rich region of 12 nucleotides. The nucleotide sequences of the L (TGCCTAACA) and R (TACGTA ATA) repeats agree with the consensus sequence obtained from direct repeats observed in promoters of bacteriocin genes whose expression is dependent on an autoinduction mechanism (16,36,38,41).…”

Section: Resultsmentioning

confidence: 99%

Purification and Genetic Characterization of Plantaricin NC8, a Novel Coculture-Inducible Two-Peptide Bacteriocin fromLactobacillus plantarumNC8

Maldonado

Ruiz-Barba

Jiménez-Dı́az

2003

Appl Environ Microbiol

155

107

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A new, coculture-inducible two-peptide bacteriocin named plantaricin NC8 (PLNC8) was isolated from Lactobacillus plantarum NC8 cultures which had been induced with Lactococcus lactis MG1363 or Pediococcus pentosaceus FBB63. This bacteriocin consists of two distinct peptides, named ␣ and ␤, which were separated by C 2 -C 18 reverse-phase chromatography and whose complementary action is necessary for full plantaricin NC8 activity. N-terminal sequencing of both purified peptides showed 28 and 34 amino acids residues for PLNC8␣ and PLNC8␤, respectively, which showed no sequence similarity to other known bacteriocins. Mass spectrometry analysis showed molecular masses of 3,587 Da (␣) and 4,000 Da (␤). The corresponding genes, designated plNC8A and plNC8B, were sequenced, and their nucleotide sequences revealed that both peptides are produced as bacteriocin precursors of 47 and 55 amino acids, respectively, which include N-terminal leader sequences of the double-glycine type. The mature ␣ and ␤ peptides contain 29 and 34 amino acids, respectively. An open reading frame, orfC, which encodes a putative immunity protein was found downstream of plNC8B and overlapping plNC8A. Upstream of the putative ؊35 region of plNC8B, two direct repeats of 9 bp were identified, which agrees with the consensus sequence and structure of promoters of class II bacteriocin operons whose expression is dependent on an autoinduction mechanism.Many lactic acid bacteria (LAB) produce antimicrobial peptides known as bacteriocins, which are directed mainly to inhibit the growth of related species or species with the same nutritive requirements (14,31,35,52). Hence, bacteriocin production seems to be aimed to compete against other bacteria which are present in the same ecological niche (4,19,20,43). It has been shown that bacteriocin production provides the producer strain with a selective advantage over other nonproducing, isogenic bacteria (46). On the other hand, it might be expected that biosynthesis of bacteriocins is a high-energyconsuming process, providing an advantage to the producer strain only if the cost/benefit ratio is favorable (19,43). It is well known that biosynthesis of proteinaceous substances is a high-energy-consuming process and for this reason is well controlled by molecular regulatory systems such as induction and catabolic repression, for instance. This suggests that bacteriocin production by LAB should also be controlled and regulated by these molecular mechanisms.Bacteria must be able to adapt their metabolism to the changing environmental conditions. This adaptation requires that bacteria sense the multitude of extracellular signals and respond by controlling the expression of an adequate repertoire of genes. Thus, bacteriocin production has been shown to be influenced by pH (1,5,6,32,34), temperature (17, 37, 54), medium composition (3, 13, 26, 27, 55), DNA-damaging agents (24,25,42), and growth conditions (8,15,28,32). However, how the presence of competing microorganisms affects production of bacteriocins is still qu...

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Section: Resultsmentioning

confidence: 99%

Purification and Genetic Characterization of Plantaricin NC8, a Novel Coculture-Inducible Two-Peptide Bacteriocin fromLactobacillus plantarumNC8

Maldonado

Ruiz-Barba

Jiménez-Dı́az

2003

Appl Environ Microbiol

155

107

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“…Such an IF acts as an indicator of the cell density, which is sensed by the corresponding HK, resulting in activation of the RR, which then activates expression of all operons necessary for bacteriocin synthesis, transport, and regulation. This quorum-sensing or autoinduction mechanism mediated by inducer peptides has been found in Carnobacterium piscicola (3,30,40,45), Lactobacillus plantarum (4,11), Lactobacillus sakei (4,12), and Enterococcus faecium (37,38). In most of these systems, however, bacteriocin production is an unstable phenotype (10,12,16,37,44).…”

mentioning

confidence: 85%

Induction of Plantaricin Production inLactobacillus plantarumNC8 after Coculture with Specific Gram-Positive Bacteria Is Mediated by an Autoinduction Mechanism

2004

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Plantaricin NC8 (PLNC8), a coculture-inducible two-peptide bacteriocin from Lactobacillus plantarum NC8, has recently been purified and genetically characterized. Analysis of an 8.1-kb NC8 DNA region downstream of the PLNC8 operon revealed the presence of at least four operons involved in bacteriocin production, showing high homology to the plantaricin cluster in L. plantarum C11. However, we found a three-component regulatory operon involving a quorum-sensing mechanism. Two of these components, the induction factor (PLNC8IF) and the histidine kinase, are novel, while the response regulator is identical to PlnD from C11. Homologous expression of plNC8IF in NC8 allowed constitutive bacteriocin production. Heterologous expression of this gene in Lactococcus lactis MG1363 produced supernatants which promoted bacteriocin production in NC8. Reverse transcription-PCR studies indicated that cocultivation of NC8 with inducing cells promoted transcription of the bacteriocin and regulatory operons in NC8. An identical result was obtained after addition of an external source of PLNC8IF. We propose that the presence of specific bacteria could act as an environmental signal that is able to switch on bacteriocin production in L. plantarum NC8 via a quorum-sensing mechanism mediated by PLNC8IF.

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“…2a), which is similar to that previously obtained in optimized medium [16]. No effect on B38 growth was observed which is in accordance with previous reports [2,23].…”

Section: Induction Of An Antibacterial Activity By Autoclaved Mrsa Cementioning

confidence: 84%

Triggering of the Antibacterial Activity of Bacillus subtilis B38 Strain against Methicillin-Resistant Staphylococcus aureus

Tabbene

Karkouch

Slimene

et al. 2010

Appl Biochem Biotechnol

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When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of dead MRSA cells in a B38 culture, increased by 8-fold the B. subtilis strain antibacterial activity reaching 160 AU/ml against MRSA strain. This antibacterial activity recovered from cell-free supernatants was stimulated by an autoinducing compound which is sensitive to the action of proteinase K suggesting a proteinaceous nature. This compound was heat-stable till 80 °C and showed a molecular mass around 20 kDa as determined by SDS-PAGE. These results suggest that the production of antibacterial compounds by B38 strain is dependent on the amount of the autoinducing compound.

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A two-component signal-transduction cascade in Carnobacterium piscicola LV17B: two signaling peptides and one sensor-transmitter

Cited by 32 publications

References 32 publications

Purification and Genetic Characterization of Plantaricin NC8, a Novel Coculture-Inducible Two-Peptide Bacteriocin fromLactobacillus plantarumNC8

Purification and Genetic Characterization of Plantaricin NC8, a Novel Coculture-Inducible Two-Peptide Bacteriocin fromLactobacillus plantarumNC8

Induction of Plantaricin Production inLactobacillus plantarumNC8 after Coculture with Specific Gram-Positive Bacteria Is Mediated by an Autoinduction Mechanism

Triggering of the Antibacterial Activity of Bacillus subtilis B38 Strain against Methicillin-Resistant Staphylococcus aureus

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