A type-specific blocking ELISA for the detection of infectious bronchitis virus antibody

Abstract

Infectious bronchitis virus (IBV) infection has been a major threat to the poultry industry worldwide. Current commercially available ELISA kits detect group-specific antibodies; however, to understand the status of field infection, a monoclonal antibody (mAb) blocking ELISA (b-ELISA) against local IBVs was developed. The selected mAb showed specificity to Taiwan IBV strains but had no cross-reactivity with the vaccine strain H120. Using the hemagglutination inhibition (HI) test as a standard, the cut-off value, sensitivity, and specificity of a b-ELISA using this mAb were evaluated in 390 field samples. The type-specificity of detection was validated using a panel of chicken hyperimmune sera. The results showed that the b-ELISA demonstrated high sensitivity (98.0%) and specificity (97.2%) of detection. The agreement between the results of the b-ELISA and the HI test was statistically significant (Kappa=0.95), and there was no significant difference between these two methods (McNemar p=0.72). The b-ELISA specifically detected Taiwan IBV serotypes but not three non-Taiwan IBV serotypes nor sera against other avian pathogens. This b-ELISA provides type-specific antibody detection of local IBV strains. It has the potential to serve as a rapid and reliable diagnostic method for IBV clinical infections in the field in Taiwan.

Development and Characterization of Neutralizing Monoclonal Antibodies Against the S1 Subunit Protein of QX-like Avian Infectious Bronchitis Virus Strain Sczy3

“…(17) The development of monoclonal antibodies (MAbs) against coronavirus is critical for improvements in clinical diagnosis. (18,19) Serological assays such as antigen capture enzyme-linked immune sorbent assay (AcELISA) can be used for antigen detection of clinical samples. (18) Specific MAb against Taiwan IBV strain 2575/98 showed specificity to Taiwan IBV strains but had no cross-reactivity with the vaccine strain H120, and the MAb was used to establish a type-specific blocking ELISA to detect Taiwan IBV infection effectively.…”

Peptides with 16R in S2 protein showed broad reactions with sera against different types of infectious bronchitis viruses

“…To detect IBV antibodies as accurately and comprehensively as possible, many ELISA antibody detection methods have been established (Chen et al, 2011;Ding et al, 2015;Lei et al, 2017). All of these methods have good efficacy, but still have some limitations.…”

Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection

“…Avian coronavirus IBV strain 2575/98 was propagated in 10-dayold specific-pathogen-free (SPF) chicken embryos via the allantoic route as previously described [23]. The virus titers of IBVs were determined with the method of Reed and Muench [24] in SPF chicken embryos and expressed as 50% embryo infectious dose (EID 50 ) [25].…”

“…The virus titers of IBVs were determined with the method of Reed and Muench [24] in SPF chicken embryos and expressed as 50% embryo infectious dose (EID 50 ) [25]. The virus-containing allantoic fluid was concentrated and purified using sucrose gradient solution as previously described to derive the native virions [23].…”