A unique neurogenomic state emerges after aggressive confrontations in males of the fish Betta splendens

Vu, Trieu-Duc; Iwasaki, Yoshiaki; Oshima, Koichiro; Chiu, Ming-Tzu; Nikaido, Masato; Okada, Norihiro

doi:10.1016/j.gene.2021.145601

Cited by 4 publications

(15 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3 B). This tendency generally reflects our previous study showing that neuronal responses were most diverse during fighting and became stable after fighting as characterized at the level of gene expression [ 25 ]. Another previous study reported that nuclear mRNA splicing via the spliceosome is one of the most enriched biological functions for the territorial male in the case of social dominance in Tripterygion delaisi fish [ 8 ].…”

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

“…We previously showed that fighting opponents develop an energy-saving strategy after a fight by reducing gene expression to a minimum among all individuals in the A0 and A30 groups [ 25 ]. We also revealed that W30 and L30 fish are tolerant of oxygen deficiency and stress based on the enrichment of hypoxia response element (HRE) motifs in some DEGs [ 25 ]. Based on our cumulative findings, we propose that both winners and losers are tolerant of oxygen deficiency and stress given not only the enrichment of HRE motifs in some DEGs but also the enrichment of IR in some other genes that are expressed in the brains of these fish.…”

Section: Discussionmentioning

confidence: 99%

“…Fish collection and experimental procedures have been described previously in our studies [ 7 , 25 ]. Briefly, several males of B. splendens (average standard length, 5.2 ± 1.1 cm) were used in this study.…”

Section: Methodsmentioning

confidence: 99%

“…For tissue preparation, males for RNA-seq were collected before fighting (B), during fighting (D20 and D60), and after fighting (A0 and A30). The RNA extraction and mRNA library preparation have been described previously in our studies [ 7 , 25 ]. Briefly, total RNA was isolated using TRIzol Reagent according to the manufacturer’s recommendation and was subsequently purified on columns with Quick-RNA MiniPrep (Zymo Research, USA).…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Alternative splicing plays key roles in response to stress across different stages of fighting in the fish Betta splendens

Oshima

Matsumura

et al. 2022

BMC Genomics

Self Cite

View full text Add to dashboard Cite

Background Aggression is an evolutionarily conserved behavior critical for animal survival. In the fish Betta splendens, across different stages of fighting interactions, fighting opponents suffer from various stressors, especially from the great demand for oxygen. Using RNA sequencing, we profiled differential alternative splicing (DAS) events in the brains of fish collected before fighting, during fighting, and after fighting to study the involvement of alternative splicing (AS) in the response to stress during the fight. Results We found that fighting interactions induced the greatest increase in AS in the ‘during-fighting’ fish, followed by that of the ‘after-fighting’ fish. Intron retention (IR) was the most enriched type among all the basic AS events. DAS genes were mainly associated with synapse assembly, ion transport, and regulation of protein secretion. We further observed that IR events significantly differentiated between winners and losers for 19 genes, which were associated with messenger RNA biogenesis, DNA repair, and transcription machinery. These genes share many common features, including shorter intron length and higher GC content. Conclusions This study is the first comprehensive view of AS induced by fighting interactions in a fish species across different stages of those interactions, especially with respect to IR events in winners and losers. Together, these findings facilitate future investigations into transcriptome complexity and AS regulation in response to stress under the context of aggression in vertebrates.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Alternative splicing plays key roles in response to stress across different stages of fighting in the fish Betta splendens

Oshima

Matsumura

et al. 2022

BMC Genomics

Self Cite

View full text Add to dashboard Cite

show abstract

The paradise fish, an advanced animal model for behavioral genetics and evolutionary developmental biology

Szabó,

Fodor,

Varga

et al. 2023

J Exp Zool Pt B

View full text Add to dashboard Cite

Paradise fish (Macropodus opercularis) is an air‐breathing freshwater fish species with a signature labyrinth organ capable of extracting oxygen from the air that helps these fish to survive in hypoxic environments. The appearance of this evolutionary innovation in anabantoids resulted in a rewired circulatory system, but also in the emergence of species‐specific behaviors, such as territorial display, courtship and parental care in the case of the paradise fish. Early zoologists were intrigued by the structure and function of the labyrinth apparatus and a series of detailed descriptive histological studies at the beginning of the 20th century revealed the ontogenesis and function of this specialized system. A few decades later, these fish became the subject of numerous ethological studies, and detailed ethograms of their behavior were constructed. These latter studies also demonstrated a strong genetic component underlying their behavior, but due to lack of adequate molecular tools, the fine genetic dissection of the behavior was not possible at the time. The technological breakthroughs that transformed developmental biology and behavioral genetics in the past decades, however, give us now a unique opportunity to revisit these old questions. Building on the classic descriptive studies, the new methodologies will allow us to follow the development of the labyrinth apparatus at a cellular resolution, reveal the genes involved in this process and also the genetic architecture behind the complex behaviors that we can observe in this species.

show abstract

The ornamental fighting fish is the next model organism for genetic studies

Yue

Wang

Sun

et al. 2022

Reviews in Aquaculture

View full text Add to dashboard Cite

The fighting fish Betta splendens is a freshwater species from Thailand and other Southeast Asian countries. This fish has been domesticated for 1000 years and bred for fighting, various colours, body size and fin types for 600 years. It is one of the most important fish species cultured for the world ornamental fish market. This fish is easy to culture, highly fecund and displays great morphological diversity. Its biology has been studied for over 100 years. Recently, its compact genome and transcriptomes have been sequenced. Genome editing with CRISPR/cas9 has been applied to knock out genes in this fish. Its diverse phenotypes, including colours, colour patterns, fin types, and aggressive behaviour, are complementary to those of other model animals.Therefore, this fish could be the next important model organism for studying phenotypic variation and aggressive behaviour. In this paper, we synthesized knowledge about its aquaculture, biology, genetics, genomic tools, phenotypes and novel insights on phenotypic variation, sex determination and aggression. We hope that the information described in this paper will facilitate genetic studies on phenotypic variations in aquaculture and aggressive behaviour in other species, including humans.

show abstract

A unique neurogenomic state emerges after aggressive confrontations in males of the fish Betta splendens

Cited by 4 publications

References 53 publications

Alternative splicing plays key roles in response to stress across different stages of fighting in the fish Betta splendens

Alternative splicing plays key roles in response to stress across different stages of fighting in the fish Betta splendens

The paradise fish, an advanced animal model for behavioral genetics and evolutionary developmental biology

The ornamental fighting fish is the next model organism for genetic studies

Contact Info

Product

Resources

About