2020
DOI: 10.1038/s41598-020-73958-5
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A universal method for high-quality RNA extraction from plant tissues rich in starch, proteins and fiber

Abstract: Using existing protocols, RNA extracted from seeds rich in starch often results in poor quality RNA, making it inappropriate for downstream applications. Though some methods are proposed for extracting RNA from plant tissue rich in starch and other polysaccharides, they invariably yield less and poor quality RNA. In order to obtain high yield and quality RNA from seeds and other plant tissues including roots a modified SDS-LiCl method was compared with existing methods, including TRIZOL kit (Invitrogen), Plant… Show more

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Cited by 77 publications
(62 citation statements)
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“…The yield of RNA extracted by CTAB is reduced due to the high a nity of CTAB with nucleic acids and other biopolymers [30]. Thus, modi ed CTAB method might not well suitable for acquiring RNA samples from Lilium davidii var.…”
Section: Discussionmentioning
confidence: 99%
“…The yield of RNA extracted by CTAB is reduced due to the high a nity of CTAB with nucleic acids and other biopolymers [30]. Thus, modi ed CTAB method might not well suitable for acquiring RNA samples from Lilium davidii var.…”
Section: Discussionmentioning
confidence: 99%
“…Most of the samples extracted with the original protocol had a ratio of A260/A230 below 1.8, which indicated the presence of contamination such as polysaccharides, salts, and residual extraction reagents. This problem also occurred in previous studies using soybean leaf samples by Bilgin et al (2009) and wheat seed samples by Vennapusa et al (2020), which had A260/ A230 ratios of 0.94 and 0.51, respectively. The results of the optimisation protocol in this study showed a significant improvement compared to the results of the standard protocol, which almost entirely did not meet the general standard A260/A230 ratio above 1.8.…”
Section: Resultsmentioning
confidence: 84%
“…It shows that the optimisation performed on optimised protocol does not have much effect in increasing the ratio. Previous research that used the TRIzol method in plant samples by Bilgin et al (2009) and Vennapusa et al (2020) also produced an A260/A280 ratio in the range of 1.88 to 1.98, meaning that the TRIzol original protocol alone could produce an adequate A260/A280 ratio from plant samples rich of secondary metabolites. However, the optimisation protocol is suggested if the sample does not meet the value above 1.8.…”
Section: Resultsmentioning
confidence: 97%
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