A validated LC–MS/MS method for determination of periplogenin in rat plasma and its application in pharmacokinetic study

A validated LC–MS/MS assay for the simultaneous determination of periplocin and its two metabolites, periplocymarin and periplogenin in rat plasma: Application to a pharmacokinetic study

“…Furthermore, our group has established a method for the application in pharmacokinetic study of periplogenin recently [14]. However, in this paper, our focus is on an established and validated LC-MS/MS method for the determination of periplocin, periplocymarin and periplogenin simultaneously, and the application of this method in the pharmacokinetic study http://dx.doi.org/10.1016/j.jpba.2015.06.008 0731-7085/© 2015 Elsevier B.V. All rights reserved.…”

A Review on Phytochemistry and Pharmacology of Cortex Periplocae

“…Li et al [25] analyzed the fatty acid compositions in the Cortex Periplocae extract using a gas chromatography-mass spectrometer, and identified 14 types of fatty acids (40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53), mainly including palmitic acid, linoleic acid, oleic acid, and linolenic acid. Shi et al isolated 15 types of volatile oils (57)(58)(59)(60)(61)(62)(63)(64)(65)(66) from Cortex Periplocae and 10 of them were purified and identified [26].…”

“…As shown from the results, they found that the concentration of periplocin could only be detected at a few time points; however, its two metabolites (periplocymarin and periplogenin) reached the maximum plasma concentration after 8-10 h. The Cmax for periplocymarin and periplogenin were 1655.63 ± 404.26 ng/mL and 32.94 ± 9.16 ng/mL, respectively. Furthermore, research was done using the same method coupled with high performance liquid chromatography tandem mass spectrometry to validate the quantifying method of periplogenin in rat plasma using psoralen as an internal standard (IS) [48]. All these studies provided the pharmacokinetics (PK) information of the toxic compounds in Cortex Periplocae.…”

A Review on Phytochemistry and Pharmacology of <em>Cortex Periplocae</em>

“…As shown from the results, they found that the concentration of periplocin could only be detected at a few time points; however, its two metabolites (periplocymarin and periplogenin) reached the maximum plasma concentration after 8-10 h. The Cmax for periplocymarin and periplogenin were 1655.63 ± 404.26 ng/mL and 32.94 ± 9.16 ng/mL, respectively. Furthermore, research was done using the same method coupled with high performance liquid chromatography tandem mass spectrometry to validate the quantifying method of periplogenin in rat plasma using psoralen as an internal standard (IS) [50]. All these studies provided the pharmacokinetics (PK) information of the toxic compounds in Cortex Periplocae.…”