In this paper, a rotational speed measuring method is proposed on the basis of a laser mouse sensor. A target disc, which reflects the laser beam, is mounted on the shaft of a motor under test. The laser mouse sensor is coupled with the target disc with an air gap. The change of the air gap between the laser sensor and target disc can be sampled with the laser mouse sensor ADNS-9800 of Avago/PixArt, so that the rotational speed from 50 RPM to 6000 RPM can be determined by using Fast Fourier Transform. The laser mouse sensor PLN-2037 of Philips uses displacement counts in order to determine the tangential speed, which is proportional to the rotational speed. This sensor can be used for measuring the speed from 10 RPM to 50 RPM. A combination of both sensors ADNS-9800 and PLN-2037 will provide a measuring range from 10 RPM to 6000 RPM.