A voltammetric assay for microRNA-25 based on the use of amino-functionalized graphene quantum dots and ss- and ds-DNAs as gene probes

“…The proposed method can distinguish the microRNA-541 sequence from the non-complementary sequence with the single-base mismatch at recognition sites. In addition, a voltammetry method based on amino functionalized GQDs (NH 2 -GQDs) was constructed to detect microRNA-25 [22]. In this work, the NH Copyright 2019 American Chemical Society.…”

Section: D-qd-based Electrochemical Dna Sensorsmentioning

confidence: 99%

“…Compared to other nanomaterials, two-dimensional nanomaterials have large specific surface area and excellent optical/electrical properties, making them the preferred choice for sensor design. More importantly, due to the excellent electrical conductivity and high current response sensitivity, 2D-QDs have greatly promoted the development of electrochemical biosensors (Figure 1) [20][21][22][23][24]. Although different 2D-QDs vary in the prominent properties, they share some common features that make them promising for constructing electrochemical biosensors: (1) electrochemical activity; (2) electrical conductivity; (3) large surface-to-volume ratio; (4) ease of functionalization.…”

Section: Introductionmentioning

confidence: 99%

Two-Dimensional Quantum Dot-Based Electrochemical Biosensors

Zhang

2022

Biosensors

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Two-dimensional quantum dots (2D-QDs) derived from two-dimensional sheets have received increasing interest owing to their unique properties, such as large specific surface areas, abundant active sites, good aqueous dispersibility, excellent electrical property, easy functionalization, and so on. A variety of 2D-QDs have been developed based on different materials including graphene, black phosphorus, nitrides, transition metal dichalcogenides, transition metal oxides, and MXenes. These 2D-QDs share some common features due to the quantum confinement effects and they also possess unique properties owing to their structural differences. In this review, we discuss the categories, properties, and synthetic routes of these 2D-QDs and emphasize their applications in electrochemical biosensors. We deeply hope that this review not only stimulates more interest in 2D-QDs, but also promotes further development and applications of 2D-QDs in various research fields.

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“…The incorporation of nanomaterials in addition to signal amplification strategy significantly enhanced the sensitivity and detection limit of electrochemical miRNA biosensor, such as the modification of electrode surface with AuNPs/Ti 3 C 2 MXene, AuNPs, addition of Ag-PEI NPs as electroactive label and addition of nanoscale copper based metal organic framework assembled Pt NPs and horseradish peroxidase (Cu-NMOF@PtNPs/HRP) as catalytic nanoprobe for the detection of miR-155 [32,[74][75][76]. Similarly, AuNPs, cysteamine-capped AuNPs, amino-functionalized graphene quantum dots (GQDs), graphene oxide (GO), electrochemically-reduced graphene oxide/gold nanowires (ERGO/AuNRs), GO/AuNRs, black phosphorus nanosheets/thionine-doped copper-MOF (BPNSs/TH/Cu-MOF) and C 60 @PAMAM-MOF were applied to modify the electrode surface for sensitive detection of miR-103, miR-25, miR-34a, miR-137, miR-199a-5p, miR-3123, and miR-3675-3p [77][78][79][80][81][82][83][84][85]. Furthermore, nanomaterials also serve as electroactive label for enhancing the electrochemical signal generation.…”

Section: Detection Of Other Mirnasmentioning

confidence: 99%

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

Low

Chai

et al. 2021

Micromachines

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MicroRNAs (miRNAs) are important non-coding, single-stranded RNAs possessing crucial regulating roles in human body. Therefore, miRNAs have received extensive attention from various disciplines as the aberrant expression of miRNAs are tightly related to different types of diseases. Furthermore, the exceptional stability of miRNAs has presented them as biomarker with high specificity and sensitivity. However, small size, high sequence similarity, low abundance of miRNAs impose difficulty in their detection. Hence, it is of utmost importance to develop accurate and sensitive method for miRNA biosensing. Electrochemical biosensors have been demonstrated as promising solution for miRNA detection as they are highly sensitive, facile, and low-cost with ease of miniaturization. The incorporation of nanomaterials to electrochemical biosensor offers excellent prospects for converting biological recognition events to electronic signal for the development of biosensing platform with desired sensing properties due to their unique properties. This review introduces the signal amplification strategies employed in miRNA electrochemical biosensor and presents the feasibility of different strategies. The recent advances in nanomaterial-based electrochemical biosensor for the detection of miRNA were also discussed and summarized based on different types of miRNAs, opening new approaches in biological analysis and early disease diagnosis. Lastly, the challenges and future prospects are discussed.

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A voltammetric assay for microRNA-25 based on the use of amino-functionalized graphene quantum dots and ss- and ds-DNAs as gene probes

Cited by 21 publications

References 29 publications

Evaluation of a biosensor-based graphene oxide-DNA nanohybrid for lung cancer

Evaluation of a biosensor-based graphene oxide-DNA nanohybrid for lung cancer

Two-Dimensional Quantum Dot-Based Electrochemical Biosensors

Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA

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