A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance

Abstract

HighlightGenes that enhance resistance to the Fusarium virulence factor deoxynivalenol (DON) are targets for disease resistance breeding. This study provides direct evidence that a wheat ABCC3 enhances resistance to DON.

TaFROG encodes a Pooideae orphan protein that interacts with SnRK1 and enhances resistance to the mycotoxigenic fungus Fusarium graminearum

“…VIGS VIGS were performed as described previously (Walter et al, 2015), with some modification. The BSMV-derived VIGS vectors used in this study consisted of the wild-type BSMV ND18 a, b, g tripartite genome (Holzberg et al, 2002;Scofield et al, 2005).…”

A valuable QTL for Fusarium head blight resistance from Triticum turgidum L. ssp. dicoccoides has a stable expression in durum wheat cultivars

“…As genomic data accumulates differences between FHB-resistant and susceptible wheat cultivars appear to be associated with early or delayed induction of defense genes involved in systemic, local responses and detoxification (Kosaka et al 2015;. The early induction of Fusarium-and DON-responsive genes in resistant genotypes has been demonstrated not only in spike samples but also in seedling tissues (Walter et al 2015), thus suggesting conserved mechanisms of resistance at both vegetative and reproductive stages. This feature of the disease allowed the development of an in vitro assay able to recognize genotypes carrying FHB resistance genes at seedling stage .…”

Deoxynivalenol resistance as a component of FHB resistance

“…TaABCC3.1 was shown to contribute to DON tolerance in wheat, as determined via enhanced DON bleaching of spikelets in plants in which the gene was silenced ( Fig. 1; Walter et al 2015). While PDR5 is likely to act as a molecular efflux pump, removing toxic substances, the function of TaABCC3.1 is unknown.…”

“…Ongoing studies are trying to delineate other orphan genes involved in wheat resistance to disease, including FHB disease. Genes associated with DON resistance and the DON response Walter et al 2008Walter et al , 2015Jia et al 2009;Schweiger et al 2013bSchweiger et al , 2016. The genes linked to Fhb1 are involved in numerous defence responses in plants, including pathogenesisrelated (PR) proteins, the synthesis of antimicrobial compounds, antioxidative stress responses, DON detoxification, cell morphogenesis and cell wall fortification (Walter and Doohan 2011;Foroud et al 2012;Schweiger et al 2016).…”