AB-Kefir Reduced Body Weight and Ameliorated Inflammation in Adipose Tissue of Obese Mice Fed a High-Fat Diet, but Not a High-Sucrose Diet

Abstract

Consumption of different types of high-calorie foods leads to the development of various metabolic disorders. However, the effects of multi-strain probiotics on different types of diet-induced obesity and intestinal dysbiosis remain unclear. In this study, mice were fed a control diet, high-fat diet (HFD; 60% kcal fat and 20% kcal carbohydrate), or western diet (WD; 40% kcal fat and 43% kcal carbohydrate) and administered with multi-strain AB-Kefir containing six strains of lactic acid bacteria and a Bifidobacterium strain, at 109 CFU per mouse for 10 weeks. Results demonstrated that AB-Kefir reduced body weight gain, glucose intolerance, and hepatic steatosis with a minor influence on gut microbiota composition in HFD-fed mice, but not in WD-fed mice. In addition, AB-Kefir significantly reduced the weight and size of adipose tissues by regulating the expression of CD36, Igf1, and Pgc1 in HFD-fed mice. Although AB-Kefir did not reduce the volume of white adipose tissue, it markedly regulated CD36, Dgat1 and Mogat1 mRNA expression. Moreover, the abundance of Eubacterium_coprostanoligenes_group and Ruminiclostridium significantly correlated with changes in body weight, liver weight, and fasting glucose in test mice. Overall, this study provides important evidence to understand the interactions between probiotics, gut microbiota, and diet in obesity treatment.

Bacillus toyonensis SAU-19 and SAU-20 Isolated From Ageratina adenophora Alleviates the Intestinal Structure and Integrity Damage Associated With Gut Dysbiosis in Mice Fed High Fat Diet

“…High fat diets has been reported to increase weight gain, affect immune organ weight and indices ( Jin et al, 2017 ; Li et al, 2020 ; Yoshizaki et al, 2020 ). Probiotics reduce excessive weight increase in animals fed high fat diet ( Kong et al, 2019 ; Chen Y. T. et al, 2021 ). From the results of the in vivo experiments, we observed that, Bacillus toyonensis SAU-19 and SAU-20 could reduce weight gain in mice compared to the HF group.…”

The Effects of Kefir and Kefir Components on Immune and Metabolic Physiology in Pre-Clinical Studies: A Narrative Review

“…However, fatty streaking of the aorta was not different between groups [59]. Kefir containing several strains of lactobacilli reduced body weight gain, insulin resistance, and hepatic steatosis in mice fed a high-fat diet [60]. Complementary results were seen in an experiment in which three different isolates (Lactobacillus acidophilus LA15.…”

Intestinal Flora Mediates Antiobesity Effect of Rutin in High‐Fat‐Diet Mice

“…In total, 12 genera correlated significantly with at least one obesity parameter (Figure 4K). The relative abundances of Roseburia, [ 33 ] Anaerovorax, Ruminiclostridium, [ 34 ] Tyzzerella, [ 35 ] and Lachnospiraceae_NK4A136_group correlated positively with the levels of serum transaminases, LPS, and liver weight and negatively with TJ gene expression levels. However, the relative abundances of Parasutterella and Akkermansia [ 36 ] correlated positively with TJ gene expression.…”