Aberrant oestrogen receptor species in human meningioma tissue

Koehorst, S. G. A.; Jacobs, Heinz; Tilanus, Marcel G.J.; Bouwens, Anne G.M.; Thijssen, J. H. H.; Blankenstein, Marinus A.

doi:10.1016/0960-0760(92)90187-n

Cited by 25 publications

(14 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Preliminary data from Koehorst et al (20) noted that a PCR product similar in size to A4, but no sequence data were provided to confirm this. We have not detected any of these isoforms in normal tissues under conditions where A4 is readily apparent (J.K.S., unpublished results).…”

Section: Discussionmentioning

confidence: 99%

Identification of an isoform of the estrogen receptor messenger RNA lacking exon four and present in the brain.

Skipper

Young

Bergeron

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

An isoform of the estrogen receptor messenger RNA (ER-mRNA) was identified in RNA from the brain of lizards and rats. Poly(A)+ RNA from brain and uteri was reverse transcribed using gene-specific primer for the ER. The resulting complementary DNA was amplified in a polymerase chain reaction followed by cloning and sequencing of the amplified products. This isoform lacks exon four and is designated A4 ER-mRNA. Although several isoforms of the ER have been described from cancerous cells, to our knowledge, none has been identified previously in the brain. Furthermore, the A4 isoform is the only isoform detected in normal tissue. The A4 isoform appeared most abundant in RNA from brain tissue, whereas uterine RNA contained only trace amounts of the isoform. Apparently, tissue-specific alternative splicing accounts for these differences in abundance. Because exon four encodes a part of the steroid-binding domain, we predict that the corresponding protein encoded by the isoform will not bind estradiol and may therefore belong to a growing subclass of the steroid/thyroid/vitamin superfamily known as orphan receptors. We predict that the putative A4 protein may function as a ligand-independent transcription factor that acts on the same DNA response elements as the conventional ER. The abundance of this isoform in the brain relative to the uterus raises fundamental questions regarding the regulation of estrogenresponsive genes in different tissues.The estrogen receptor (ER) is a member of the steroid/ thyroid/vitamin superfamily of nuclear receptors. The most thoroughly studied members of this family function as ligandactivated transcription factors that bind to sequence-specific response elements in the DNA to modulate transcription of specific sets of genes (for reviews, see refs.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of an isoform of the estrogen receptor messenger RNA lacking exon four and present in the brain.

Skipper

Young

Bergeron

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…This variant ER mRNA has also been observed in MCF7 cells (14) and in human meningioma tissues (23 (26), breast cancer tumor cells (27), and cells of the osteoblast lineage (2,4). Covalent labeling and immunodetection of nuclear ERs (21) revealed an entity with an apparent molecular weight of 65,000, a typical size for ER.…”

mentioning

confidence: 99%

“…1). The 500-bp band has been described in MCF7 cells (14) and in human meningioma tissues (23 (Fig. 6).…”

mentioning

confidence: 99%

Functional estrogen receptors in a human preosteoclastic cell line.

Fiorelli

Gori

Petilli

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The primary biological effect of the estrogen estradiol-17P (17PE2) on bone is to decrease bone resorption. decreasing bone resorption (1). It has, therefore, been proposed that estrogen acts indirectly on osteoclast differentiation or function through its effects on osteoblasts, bone marrow stromal cells (6, 7), and bone endothelium (8).More recent evidence has accumulated that suggests a direct effect of 1713E2 on mature osteoclasts, by the demonstration of functional ERs in mature osteoclasts (9-11).To evaluate the possible direct action of 17f3E2 on osteoclastogenesis, we used the human leukemic cell line FLG 29.1, which is induced by phorbol esters to express many features typical of osteoclasts (12

show abstract

“…We recently reported on the detection of mRNA coding for the wild type receptor by using the reversed-transcriptase-PCR technique (Koehorst et al, 1992). Apart from the wild type (wt) mRNA we also found two alternatively spliced mRNA's.…”

mentioning

confidence: 99%

“…The ERE-protein complex from the meningiomas co-migrates with the MCF7 ER-ERE complex even in gels run for longer times (data not shown). To check the integrity of the isolated protein all the meningiomas were tested for SpI binding and all except one were found to be positive (Figure 3, lower (Koehorst et al, 1992). Besides the wild type transcript two variants were detected, one missing exon 7 and the other missing exon 4 (Koehorst et al, 1993).…”

mentioning

confidence: 99%

Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE)

Koehorst

Jacobs²,

Thijssen³

et al. 1993

Br J Cancer

View full text Add to dashboard Cite

When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable. A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay. The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein. Images Figure 1 p291-b Figure 2 Figure 3

show abstract

Aberrant oestrogen receptor species in human meningioma tissue

Cited by 25 publications

References 13 publications

Identification of an isoform of the estrogen receptor messenger RNA lacking exon four and present in the brain.

Identification of an isoform of the estrogen receptor messenger RNA lacking exon four and present in the brain.

Functional estrogen receptors in a human preosteoclastic cell line.

Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE)

Contact Info

Product

Resources

About