Abietic acid ameliorates psoriasis-like inflammation and modulates gut microbiota in mice

Li, Xiao Qiang; Chen, Yong; Dai, Guan Cheng; Zhou, Bin; Yan, Xiaoning; Tan, Ren Xiang

doi:10.1016/j.jep.2021.113934

Cited by 24 publications

(13 citation statements)

References 30 publications

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“…Terpenoid natural compounds have novel and diverse molecular structures and a wide range of biological activities, and are important structural sources for drug research and development [23,24], such as vinca alkaloids, paclitaxel, camptothecin and so on. Abietic acid is a natural tricyclic diterpenoid compound derived from rosin, which has been found to have anti-inflammatory, antibacterial and antiviral activities [18,19], and is a suitable drug candidate for sepsis treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Abietic acid (AA) is a natural terpenoid compound that has been shown to have various biological activities such as antitumor, antibacterial, antiviral and anti-inflammatory [18,19]. In vitro, previous studies have found that AA could lipopolysaccharide-induced macrophage inflammation [20,21], but its specific mechanism of action is not clear, and whether it is related to macrophage polarization is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Abietic acid attenuates sepsis-induced lung injury by inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway to inhibit M1 macrophage polarization

et al. 2022

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Lung injury is one of the leading causes of death in sepsis. Abietic acid (AA) has demonstrated antiinflammatory and bacteriostatic properties. Herein, we established a mouse model of sepsis by cecal ligation and puncture, and intraperitoneally injected AA to treat. Lung injury was assessed by H&E staining and the inflammation in bronchoalveolar lavage fluid (BALF) were assessed by counting the number of inflammatory cells and detecting the content of inflammatory factors. Meanwhile, we also designed to study the effect of AA on lipopolysaccharide (LPS)-induced inflammatory response and macrophage marker gene expression in RAW264.7 cells in vitro. In this study, we found that AA inhibited LPS-induced secretion of inflammatory mediators (IL-1β, TNF-α, IL-6 and MIP-2), and decreased the expression of M1 macrophage e markers (CD16 and iNOS) and p-p65 protein, while increased the expression of M2 macrophage markers (CD206 and Arg-1) in RAW264.7 cells in vitro. In vivo, the therapy of AA not only rescued septic animals, but also attenuated lung injury in sepsis mice. Moreover, AA decreased the number of total cells, neutrophils and macrophages, the conceration of total protein, and the levels of inflammatory mediators in BALF of sepsis mice. Further, we found that AA inhibited M1 macrophage polarization and blocked NF-κB pathway in BALF of sepsis mice. In conclusion, Abietic acid attenuates sepsis-induced lung injury, and its mechanism may be related to reducing inflammation by inhibiting NF-κB signaling to inhibit M1 macrophage polarization.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Abietic acid attenuates sepsis-induced lung injury by inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway to inhibit M1 macrophage polarization

et al. 2022

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“…The severity of dorsal skin lesions was assessed by the psoriasis area and severity index (PASI). 22 The erythema, scaling, and thickening were independently scored from 0 to 4 as follows: 0, none; 1, slight; 2, moderate; 3, marked; 4, very marked. The cumulative score (erythema, scaly, thickening) is used as a measure of the severity of skin damage.…”

Section: Methodsmentioning

confidence: 99%

Effect of Total Glucosides of Paeony on Imiquimod-Induced Psoriatic Skin Lesions by Regulating VEGF

Zhang¹,

Zhou²,

Sun³

2021

CCID

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Purpose The purpose of this study was to use a murine model of psoriasis to examine the effect of total glycosides of paeony (TGP) on psoriatic skin lesions and on the expression of vascular endothelial growth factor (VEGF) in skin lesions and blood. Methods A murine model of psoriasis was produced by shaving the backs of the mice and applying 5% imiquimod cream, 50 mg, to the backs of the mice once a day. Mice were killed on day 8, and skin and blood samples were obtained for histopathological examination and analysis of VEGF mRNA expression. Results By day 8 of the application of imiquimod cream, skin lesions characteristic of psoriasis were evident, and histopathological examination of skin sections showed changes consistent with psoriasis (corneum thickening and parakeratosis, attenuation of the stratum granulosum, thickening of the stratum spinosum, and lengthening of the epidermal ridge). In the treatment group, 7 days of treatment with TGP resulted in resolution of the skin lesions, and histopathological examination showed the epidermis and dermis are approximately normal, without corneum thickening, hyperkeratosis, and parakeratosis. On day 7 of treatment, skin expression of VEGF mRNA was significantly lower in the treatment group than in the group that did not receive treatment (p < 0.05). Blood VEGF mRNA expression was not different between the groups. Conclusion TGP is effective for the treatment of psoriasis and may act by decreasing lesion VEGF mRNA expression.

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“…The osteoprotective effect of AA was detected in receptor activator of nuclear factors κB ligand (RANKL)-induced osteoclastogenesis and in lipopolysaccharide (LPS)-induced osteolysis models [ 19 ], while its mitigation effects on nephropathy progression were detected in the kidney in type-2 diabetes mellitus (DM) [ 1 ]. Furthermore, AA significantly ameliorated psoriasis-like skin inflammation in imiquimod (IMQ)-treated BALB/c mice, interleukin (IL)-1β-induced inflammation in human osteoarthritic chondrocytes, airway inflammation in an allergic asthma model, and kidney inflammation in a type-2 DM model [ 1 , 20 , 21 , 22 ]. However, whether AA has anti-atopic dermatitis (AD) effects in the DNCB-induced AD model remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Anti-Atopic Dermatitis Effects of Abietic Acid Isolated from Rosin under Condition Optimized by Response Surface Methodology in DNCB-Spread BALB/c Mice

et al. 2023

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Abietic acid (AA) is known to have beneficial effects on inflammation, photoaging, osteoporosis, cancer, and obesity; however, its efficacy on atopic dermatitis (AD) has not been reported. We investigated the anti-AD effects of AA, which was newly isolated from rosin, in an AD model. To achieve this, AA was isolated from rosin under conditions optimized by response surface methodology (RSM), and its effects on cell death, iNOS-induced COX-2 mediated pathway, inflammatory cytokine transcription, and the histopathological skin structure were analyzed in 2,4-dinitrochlorobenzene (DNCB)-treated BALB/c mice after treatment with AA for 4 weeks. AA was isolated and purified through isomerization and reaction-crystallization under the condition (HCl, 2.49 mL; reflux extraction time, 61.7 min; ethanolamine, 7.35 mL) established by RSM, resulting in AA with a purity and extraction yield of 99.33% and 58.61%, respectively. AA exhibited high scavenging activity against DPPH, ABTS, and NO radicals as well as hyaluronidase activity in a dose-dependent manner. The anti-inflammatory effects of AA were verified in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages through amelioration of the inflammatory response, including NO production, iNOS-induced COX-2 mediated pathway activation, and cytokine transcription. In the DNCB-treated AD model, the skin phenotypes, dermatitis score, immune organ weight, and IgE concentration were significantly ameliorated in the AA cream (AAC)-spread groups compared to the vehicle-spread group. In addition, AAC spread ameliorated DNCB-induced deterioration of skin histopathological structure through the recovery of the thickness of the dermis and epidermis and the number of mast cells. Furthermore, activation of the iNOS-induced COX-2 mediated pathway and increased inflammatory cytokine transcription were ameliorated in the skin of the DNCB+AAC-treated group. Taken together, these results indicate that AA, newly isolated from rosin, exhibits anti-AD effects in DNCB-treated AD models, and has the potential to be developed as a treatment option for AD-related diseases.

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Abietic acid ameliorates psoriasis-like inflammation and modulates gut microbiota in mice

Cited by 24 publications

References 30 publications

Abietic acid attenuates sepsis-induced lung injury by inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway to inhibit M1 macrophage polarization

Abietic acid attenuates sepsis-induced lung injury by inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway to inhibit M1 macrophage polarization

Effect of Total Glucosides of Paeony on Imiquimod-Induced Psoriatic Skin Lesions by Regulating VEGF

Anti-Atopic Dermatitis Effects of Abietic Acid Isolated from Rosin under Condition Optimized by Response Surface Methodology in DNCB-Spread BALB/c Mice

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