Ablation of liver cancer cells in vitro by a plasma needle

Abstract

A plasma needle using a dielectric barrier discharge reactor at atmospheric pressure with a funnel-shaped nozzle was developed. The preliminary characteristics of the plume and applications to the ablation of cultured human hepatocellular carcinoma (HCC) BEL-7402 cell line were presented. The effect of oxygen, which was injected into argon plasma afterglow region through a steel tube, was studied. The efficiency of argon-oxygen plasma depends sensitively on the oxygen concentration in argon plasma. Large differences between spectra in atmosphere and those in Dulbecco’s modified eagle medium are found. It is found that ultraviolet rays, O, OH, and Ar radicals can reach the bottom of solution and act on HCC cells and there is an optimum input power to get the most radicals.

Low temperature atmospheric pressure plasma sources for microbial decontamination

“…But also emerging application areas like plasma healthcare can be approachable by the usage of non-LTE plasmas (Stoffels, 2007;Kong et al, 2009;Lloyd et al, 2010;Laroussi, 2009;Fridman et al, 2008). Thus, plasma treatment of cancer cells (Kim, Kim, Park, Jeon, Seo, Iza and Lee, 2009;Vandamme et al, 2010;Zhang et al, 2008), living human cells (Tümmel et al, 2007;Stoffels et al, 2008;Yonson et al, 2006), prevention of nosocomial infections and the therapy of infected wounds (Fridman et al, 2008;Isbary et al, 2010) as a few examples are in the ongoing research focus.…”

Induction of cell growth arrest by atmospheric non-thermal plasma in colorectal cancer cells

“…It has been reported that plasma induces power-and timedependent cell death in several cell lines (Montie et al, 2000;Stoffels et al, 2006;Zhang et al, 2008). A gas-only treatment (helium and oxygen) was used to exclude the gas effects of nonthermal atmospheric plasma.…”

“…Studies investigating the interaction of plasma jets with living cells have shown eradication of pathogens (Goree et al, 2006;Kolb et al, 2008;Stoffels et al, 2006), blood coagulation Kalghatgi et al, 2007;Kieft et al, 2005), tissue sterilization Shashurin et al, 2008), and the ablation of cultured liver cancer cells (Zhang et al, 2008). The effects of cold atmospheric plasma were shown to be due not to heat, but to active species, mainly oxygen/hydroxyl radicals and nitric oxide, generated in the plasma or in the tissue brought into contact with the plasma (Chen et al, 2007).…”

“…Cell detachment was shown to be a reversible effect that allows removal or mobilization of cells (Kieft et al, 2006;Stoffels et al, 2006). Another mechanism generated by plasma is the induction of apoptosis (Montie et al, 2000;Nuccitelli et al, 2009;Shashurin et al, 2008;Zhang et al, 2008). Cells undergoing plasma-induced apoptosis usually develop characteristic morphological changes, including nuclear condensation and degradation of DNA into oligonucleosomal fragments.…”

“…Two important cell responses to plasma treatment have been identified: temporary loss of cell adhesion and programmed cell death (Kim et al, 2008(Kim et al, , 2009Stoffels et al, 2006;Zhang et al, 2008). Treatment with plasma allowed for localized cell detach-ment (Kieft et al, 2006).…”