Abnormal Expression of Long Noncoding RNAs in Primary Immune Thrombocytopenia: A Microarray Related Study

Abstract

Background/Aims: Long noncoding RNAs (lncRNAs) are important regulators of biological processes and they contribute to the pathological developments of various diseases, including autoimmune diseases. To gain the further understanding, we estimate the expression of lncRNAs in primary immune thrombocytopenia (ITP). Methods: In this study, microarray studies were performed to characterize expression profiles of various lncRNAs and mRNAs in blood samples collected from ITP patients. Quantitative real-time PCR (qRT-PCR) was performed to confirm the results, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and gene ontology analysis were used to provide functional annotations, co-expression network construction (CNC) analysis was made to reveal the relations between lncRNAs and their targeted genes. Results: A total of 1177 and 632 lncRNAs were significantly up-regulated or down-regulated, respectively, in “newly diagnosed ITP” patients versus healthy individuals. In addition, 1182 genes and 737 genes were up-regulated or down-regulated, respectively, in “chronic recurrent ITP” patients versus healthy individuals. In a KEGG analysis, “TNF signaling pathway-Homo sapiens (human)” was a key result. In a gene ontology analysis, “Granulocyte macrophage colony-stimulating factor production (GO: 0032604, ontology: Biological process, P = 1.69577E-05)” and “coreceptor activity (GO: 0015026, ontology: molecular function, P = 4.67594E-06)” were the two most critical results. Data from qRT-PCR and receiver operating characteristic curves further demonstrated that ENST00000440492, ENST00000528366, NR_038920, and ENST00000552576 can efficiently distinguish different stages of ITP, especially NR_038920 and ENST00000528366. In a CNC analysis, four lncRNAs were emphasized, and NR_038920 and ENST00000528366 were both associated with proteins with important roles in autoimmune diseases. Conclusions: These results suggest that lncRNAs act through targeted genes to mediate their functions and to mediate their functions and affect the pathogenesis of ITP.

Autoimmune Cytopenias in Chronic Lymphocytic Leukemia: Focus on Molecular Aspects

“…TNF and granulocyte macrophage colony-stimulating factor signaling were the most interested pathways. Interestingly, lncRNAs ENST00000440492, ENST00000528366, NR_038920, and ENST00000552576 were able to distinguish newly diagnosed from chronic ITP (120). Finally, Peng et al used gene expression profiling analysis and whole-exome sequencing on samples from family members with ITP, sporadic ITP cases and healthy individuals and identified a potential pathologic p.G76S heterozygous mutation on the TNFRSF13B gene.…”

Decreasing lncRNA PVT1 causes Treg/Th17 imbalance via NOTCH signaling in immune thrombocytopenia

“…Over the past few years, it has been reported that lncRNAs are involved in the pathogenesis of ITP [23]. Ayoub et al indicated that lncRNAs metastasis associated in lung denocarcinoma transcript 1 (MALAT1) and THRIL could be potential markers of ITP [24].…”

LncRNAs LY86-AS1 and VIM-AS1 Distinguish Plasma Cell Leukemia Patients from Multiple Myeloma Patients

“…Thrombocytopenia is a common sign of MM [ 64 ]. In the study by Li et al [ 65 ], a relation between autoimmune idiopathic thrombocytopenic purpura and an increased level of lncRNA was described. It is suggested that lncRNAs affect and mediate the functions of the gene network in this disease [ 65 ].…”