Absence of Minocycline in Sebum?

Aubin, F.; Blanc, Dominique; Guinchard, C.; Agache, P

doi:10.1111/j.1346-8138.1989.tb01282.x

Cited by 11 publications

(3 citation statements)

References 22 publications

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“…The prevalence of SD is 11.6% in USA [6] but can be higher in elderly people (over 80%) [5] and in Parkinsonism (52–59.5%) [8]. In recent years, a few studies concerning SD and PD have been published [18-20]. To our best knowledge, this is the first culture-based epidemiology study performed on patients with SD and PD.…”

Section: Discussionmentioning

confidence: 99%

A laboratory-based study on patients with Parkinson’s disease and seborrheic dermatitis: the presence and density of Malassezia yeasts, their different species and enzymes production

Arsenijevic

Milobratović²,

Barać

et al. 2014

BMC Dermatol

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BackgroundSeborrheic dermatitis (SD) and Parkinson’s disease (PD) are frequently associated conditions. Aims of this study were: to determine severity of SD, presence of different species and density of Malassezia yeasts; to assess yeast lipases and phosphatases production in vitro and to compare these results between SD patients with and without PD.MethodsThis case–control prospective study was conducted at the Dermatology and Neurology Units, Clinical Centre of Serbia and at the National Medical Mycology Reference Laboratory, University of Belgrade Medical School, Serbia. A total of 90 patients and 70 healthy controls (HC) were investigated: 60 patients with SD (SDN) and 30 patients with SD and PD (SDP). Culture-based mycological examination was carried out on lesional skin (LS) and non-lesional skin (NLS). A yeasts density was determined by counting the Malassezia colony forming units per tape (CFU/tape). Enzymes production by isolated Malassezia was investigated.ResultsThe most patients with SD were male (76.7%; SDP and 63.3%; SDN) and the intensity of SD was dominantly severe or moderate (76.7%; SDP and 75%; SDN). The presence of Malasseziа was high on LS in both groups (87.3%; SDP and 86.7%; SDN) (p=0.667).The highest yeasts density (mean CFU/tape=67.8) was detected on LS in 53% of SDP group and in 21.7% of SDN group (mean CFU/tape=31.9) (p < 0.01). The presence of negative cultures was lower in SDP group (13.3%) in comparison to HC and SDN groups (37% and 31.7%, respectively). Malassezia density on NLS in SDP group (mean CFU/tape=44.3) was significantly higher in comparison to SDN and HC (p=0.018). M. globosa was the most abundant species identified amongst isolates from the SDP group (42.3%) and exhibited high production of phosphatase and lipase in vitro.ConclusionFrom this laboratory-based study a positive correlation between SD, PD, M. globosa incidence, high yeast density and high phosphatase and lipase activity was established. Our data lead to conclusion that local skin performance of PD patient’s characterized with increased sebum excretion ratio play a role in SD by stimulation of yeasts replication and enzyme production.

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Section: Discussionmentioning

confidence: 99%

A laboratory-based study on patients with Parkinson’s disease and seborrheic dermatitis: the presence and density of Malassezia yeasts, their different species and enzymes production

Arsenijevic

Milobratović²,

Barać

et al. 2014

BMC Dermatol

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“…Aubin et al . stated that minocycline was not detected in the sebum of 10 healthy volunteers receiving minocycline at 200 mg/day for 4 weeks, claiming that the effectiveness of minocycline in the treatment of acne vulgaris is not based on sebaceous secretion 27 . In a further randomized placebo‐controlled clinical study with 45 acne patients, minocycline at 100 mg/day for 28 days led to a late, subclinical increase in sebaceous excretion, as detected by lipometry 28 .…”

Section: Discussionmentioning

confidence: 99%

Minocycline suppresses lipogenesis via inhibition of p300 histone acetyltransferase activity in human SZ95 sebocytes

Shin

Zouboulis

Kim

et al. 2022

Acad Dermatol Venereol

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Background Minocycline is a second-generation tetracycline drug, which is widely used to treat diverse infectious and inflammatory diseases such as acne vulgaris. The effects of minocycline on acne vulgaris have been mainly attributed to its anti-inflammatory effect; however, its sebum-regulating effect and the relevance to epigenetic regulation in human sebaceous glands remain uninvestigated.Objectives To identify the potential underlying epigenetic mechanism of sebum-inhibitory effects of minocycline in human SZ95 sebocytes.Methods The quantity of lipid droplets and the expression of key lipogenic genes were analysed in minocyclinetreated SZ95 sebocytes. To examine whether the sebum-inhibitory effects of minocycline are relevant to histone acetylation, we analysed the effects of minocycline on p300 HAT and total HDAC activity. To elucidate the functional implication of p300 HAT inhibition by minocycline in sebocytes, we assessed the effect of p300 knockdown, inhibition and overexpression on lipid accumulation in SZ95 sebocytes.Results Minocycline suppressed the insulin and liver X receptor agonist-induced lipid accumulation and the expression of the key lipogenic transcription factor sterol regulatory element-binding protein 1 (SREBP1) and its downstream genes, fatty acid synthase (FAS) and acetyl-CoA carboxylase a (ACCa). Minocycline inhibited p300 HAT activity in a concentration-dependent manner, but demonstrated no effect on global HDAC activity, resulting in a significant decrease in histone acetylation. Downregulation of p300 by knockdown or inhibition significantly suppressed SREBP1 expression, histone acetylation and lipid accumulation, whereas p300 overexpression enhanced these effects. Moreover, p300 overexpression rescued minocycline-inhibited SREBP1 expression and lipid synthesis. Conclusions Our findings revealed a novel sebum-regulating effect of minocycline. Moreover, as p300 HAT is a key epigenetic regulator of sebaceous lipogenesis, its inhibitors could be used for the treatment of acne vulgaris.

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“…It might be assumed that significant concentrations of minocycline would be present in sebaceous follicles. However, minocycline has only been found in cutaneous surface lipids and not in the sebum [2, 3]. The purpose of the study was to investigate the comedonal diffusion of minocycline in acne volunteers, using high-performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%