Absorption and excretion of conjugated flavonols, including quercetin-4′-O-β-glucoside and isorhamnetin-4′-O-β-glucoside by human volunteers after the consumption of onions

Aziz, Azlina Abdul; Edwards, Christine; Lean, Michael E. J.; Crozier, Alan

doi:10.1080/10715769800300291

Cited by 175 publications

(89 citation statements)

References 22 publications

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“…These data supported previous data of 6% quercetin bioavailability, t max = 0.68 h and t 1/2 = 10.9 h, after 12 human subjects ingested 331 μmol of quercetin in onions (28), a dose comparable with our study (381 μmol). Lesser apparent quercetin absorption of 1% was shown after intake of 300 g of lightly fried yellow onions in five subjects (29). The reason for the difference in these bioavailability values is not clear, but may be due to substances in the omelet or in red onions that facilitated the absorption of quercetin.…”

Section: Discussionmentioning

confidence: 94%

Greater Apparent Absorption of Flavonoids Is Associated with Lesser Human Fecal Flavonoid Disappearance Rates

Simons

Renouf

Murphy

et al. 2009

J. Agric. Food Chem.

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It was hypothesized that 5,7,4 0 -OH-flavonoids disappeared more rapidly from human fecal incubations and were less absorbable by humans than flavonoids without 5-OH moieties. Anaerobic fecal disappearance rates over 24 h were determined for 15 flavonoids in samples from 20 men and 13 women. In these anaerobic fecal mixtures, flavonoids with 5,7,4 0 -OH groups, genistein, apigenin, naringenin, luteolin, kaempferol, and quercetin (disappearance rate, k = 0.46 ( 0.10 h -1 ), and methoxylated flavonoids, hesperetin and glycitein (k = 0.24 ( 0.21 h -1 ), disappeared rapidly compared with flavonoids lacking 5-OH (e.g., daidzein, k = 0.07 ( 0.03 h -1 ). Apparent absorption of flavonoids that disappeared rapidly from in vitro fecal incubations, genistein, naringenin, quercetin, and hesperetin, was compared with that of daidzein, a slowly disappearing flavonoid, in 5 men and 5 women. Subjects ingested 104 μmol of genistein and 62 μmol of daidzein (soy milk), 1549 μmol of naringenin and 26 μmol of hesperetin (grapefruit juice), and 381 μmol of quercetin (onions) in three test meals, each separated by 1 week. Blood and urine samples were collected over 24 h after each test meal. Plasma flavonoid concentrations ranged from 0.01 to 1 μM. The apparent absorption, expressed as percentage of ingested dose excreted in urine, was significantly less for naringenin (3.2 ( 1.7%), genistein (7.2 ( 4.6%), hesperetin (7.3 ( 3.2%), and quercetin (5.6 ( 3.7%) compared with daidzein (43.4 ( 15.5%, p = 0.02). These data affirmed the hypothesis that the 5,7,4 0 -OH of flavonoids limited apparent absorption of these compounds in humans.

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Section: Discussionmentioning

confidence: 94%

Greater Apparent Absorption of Flavonoids Is Associated with Lesser Human Fecal Flavonoid Disappearance Rates

Simons

Renouf

Murphy

et al. 2009

J. Agric. Food Chem.

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“…Interestingly, the relative urinary excretion of naringenin from grapefruit juice seems higher than that of hesperetin from orange juice: 8.9% (Fuhr & Kummert, 1995), Bioavailability of flavanones in humans C Manach et al 14% (Lee & Reidenberg, 1998), 30% (Erlund et al, 2001). Only a few data have been published for flavonols and flavanols, but their relative urinary excretion seems slightly lower than that of flavanones: 2% for tea catechins (Lee et al, 1995) and 0.3 -1.4% for quercetin (Hollman et al, 1997;Aziz et al, 1998). In contrast, the percentage of ingested isoflavones that are excreted in urine is markedly higher than that of other flavonoids: 10 -20% for genistein and 20 -50% for daidzein (Xu et al, 1994(Xu et al, , 2000King & Bursill, 1998).…”

Section: Discussionmentioning

confidence: 99%

Bioavailability in humans of the flavanones hesperidin and narirutin after the ingestion of two doses of orange juice

Manach¹,

Morand²,

et al. 2003

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Objective: Flavanones are polyphenols specific of citrus fruits, where they are present in high amounts. Although citrus fruits and juices are widely consumed in the world, little information has been published on flavanone bioavailability in humans. The aim of the present study was to determine the nature of the circulating metabolites, the plasma kinetics and the urinary excretion patterns of the flavanones, hesperidin and narirutin. Design: After an overnight fast, five healthy volunteers ingested 0.5 or 1 l of a commercial orange juice providing 444 mg=l hesperidin and 96.4 mg=l narirutin, together with a polyphenol-free breakfast. Blood was sampled at 10 different timepoints over a 24 h period. Urine was collected for 48 h, in five fractions. Results: Flavanones metabolites appeared in plasma 3 h after the juice ingestion, reached a peak between 5 and 7 h, then returned to baseline at 24 h. The peak plasma concentration of hesperetin was 0.46 AE 0.07 mmol=l and 1.28 AE 0.13 mmol=l after the 0.5 and 1 l intake, respectively. It was lower for naringenin: 0.20 AE 0.04 mmol=l after the 1 l dose. The circulating forms of hesperetin were glucuronides (87%) and sulphoglucuronides (13%). For both flavanones, the urinary excretion was nearly complete 24 h after the orange juice ingestion. The relative urinary excretion was similar for hesperetin and naringenin and did not depend on the dose: values ranged from 4.1 AE 1.2 to 7.9 AE 1.7% of the intake. Conclusions: In case of a moderate or high consumption of orange juice, flavanones may represent an important part of the pool of total polyphenols present in plasma.

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“…Several studies have provided information on the bioavailability of¯avonoids from a food matrix, however they give no indication of the potential antioxidant activity associated with this absorption (Hollman et al, , 1996b(Hollman et al, , 1997aAziz et al, 1998). We have therefore examined the effects of long-term rutin (quercetin-3-O-brutinoside) supplementation in human volunteers.…”

Section: Discussionmentioning

confidence: 99%

“…Acid hydrolysis of rutin (to yield quercetin) facilitated post-column derivatization with aluminium nitrate enabling¯uorimetric detection which afforded signi®cantly enhanced assay sensititivity (Hollman et al, 1996a). Acid hydrolysis of plasma was performed in 50% methanol containing 20 mmolal diethyldithiocarbamate and 1.2 M HCl (Aziz et al, 1998). Plasma ascorbic acid levels in metaphosphoric acid-treated samples were determined by reverse-phase HPLC with UV detection at 263 nm (Ross, 1994) Determination of plasma retinol, a-tocopherol, g-tocopherol and six carotenoids was by reverse-phase HPLC with simultaneous UV and¯uorimetric detection (Hess et al, 1991).…”

Section: Methodsmentioning

confidence: 99%

Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study

Boyle¹,

Dobson²,

Duthie³

et al. 2000

Eur J Clin Nutr

179

107

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Objective: To determine the potential antioxidant effect of rutin (quercetin-3-O-b-rutinoside) supplementation. Design: A 6-week randomized single-blind placebo controlled trial was conducted; 500 mg rutin supplement was compared to an equivalent amount of glucose placebo. In addition, a pharmacokinetic study was carried out. Setting: The Rowett Research Institute, Aberdeen, UK. Subjects: Eighteen healthy non-obese normocholesterolaemic female volunteers in the age range 18 ± 48 y. Main outcome measures: Plasma¯avonoids, ascorbic acid, tocopherols and carotenoids, plasma antioxidant capacity, lymphocyte DNA damage, blood chemistry and haematology, liver function tests, urinary malondialdehyde, 8-hydroxy-2H -deoxyguanosine and 8-iso-prostaglandin F 2a . Results: Eighteen volunteers completed the trial. Rutin supplementation did not induce any adverse changes in blood chemistry or indices of liver function. Plasma¯avonoids were signi®cantly elevated in the rutinsupplemented group. Endogenous oxidation of pyrimidines was signi®cantly decreased in both rutin-and placebo-treated volunteers. There was no signi®cant change in the level of urinary 8-hydroxy-2 H -deoxyguanosine or urinary malondialdehyde in either group. A linear correlation was observed between urinary malondialdehyde and urinary 8-iso-prostaglandin F 2a (R 0.54, P`0.01). Conclusion: Six weeks' rutin supplementation signi®cantly elevated the levels of three plasma¯avonoids (quercetin, kaempferol and isorhamnetin) but there was no signi®cant change in plasma antioxidant status. The decrease in the level of endogenous base oxidation in lymphocyte DNA seen in both the placebo-and rutinsupplemented subjects may re¯ect seasonal changes in other dietary antioxidants.

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Absorption and excretion of conjugated flavonols, including quercetin-4′-O-β-glucoside and isorhamnetin-4′-O-β-glucoside by human volunteers after the consumption of onions

Cited by 175 publications

References 22 publications

Greater Apparent Absorption of Flavonoids Is Associated with Lesser Human Fecal Flavonoid Disappearance Rates

Greater Apparent Absorption of Flavonoids Is Associated with Lesser Human Fecal Flavonoid Disappearance Rates

Bioavailability in humans of the flavanones hesperidin and narirutin after the ingestion of two doses of orange juice

Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study

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