Acceleration in the Repair and Regenerative Responses by Different Doses of Hesperidin in the Deep Full Thickness Cutaneous Wound of Mice Whole Body Exposed To 6 Gy of γ -Radiation

Abstract

Ionizing radiations are necessary evil as everyone is exposed to them intentionally for diagnostic purposes or unintentionally from cosmic sources or background radiations. Ionizing radiations are known to cause adverse health effects at low to moderate doses whereas high doses are fatal. One of the adverse effects of ionizing radiations is the retardation of wound healing. We have studied the effect of different doses of hesperidin on the wound contraction in mice whole body exposed to 6 Gy of ͏ γ-irradiation. The mice were administered orally with a single dose of 0, 10, 20, 50, 100, 200, 250 or 500 mg/kg of hesperidin one hour before exposure to 6 Gy of γ-radiation. After 10 minutes of irradiation a full-thickness skin wound was produced on the dorsum of Swiss albino mice receiving various doses of hesperidin or not before exposure to 6 Gy γ-radiations. Assessment of wound contraction was carried out by capturing the video images of the wound on different post-irradiation days until complete healing of wounds and the mean wound healing time was determined by daily monitoring the progress of wound healing until complete closure of wounds. Administration of 10 to 500 mg/kg body weight of hesperidin resulted in a dose-dependent increase in the wound contraction when compared with non-drug treated control. A highest but significant wound contraction was observed for 100 mg/kg hesperidin at all the post-irradiation days. A complete healing of wounds was observed by day 16 postirradiation in the hesperidin treated sham-irradiation group when compared to day 18.7 sham-irradiation group. The whole-body exposure of mice to 6 Gy of γ-radiation caused a significant delay in wound contraction and increased the mean wound healing time when compared to untreated control animals. Administration of different doses of hesperidin before 6 Gy irradiation progressively increased wound contraction and a maximum effect was observed at 100 mg/kg thereafter the effect was lesser than 100 mg/kg hesperidin. A similar effect was observed on mean wound healing time where the wound healing time was reduced by approximately two days when compared to irradiation alone. Our study demonstrates that hesperidin enhances the wound contraction and reduces the mean wound healing time.

Radioprotective Effect of Hesperidin: A Systematic Review

“…A hesperidin dose of 100 mg/kg body weight was mostly used in the included studies to assess its radioprotective effect. In addition, this dose has been shown to be the most effective in reducing the healing time of radiation-induced wounds by two days [24]. Results from another study by Haddadi et al also showed that this same oral dose of hesperidin was effective in accelerating wound healing from radiation-induced skin damage [31].…”

Chemopreventive effect of hesperidin, a citrus bioflavonoid in two stage skin carcinogenesis in Swiss albino mice

“…Topical and oral administration of hesperidin were effective in the healing of clean or infected wounds [25]. Hesperidin also accelerated repair and regeneration of irradiated excision wound in mice and was found to be non-toxic up to 5 g/kg body weight in mice [26, 27, 28, 29]. Dietary administration of hesperidin at the level of 5% for 13 weeks did not induce any toxic effects in different tissues [30].…”