Acceleration of dermal wound healing by using electrospun curcumin‐loaded poly(ε‐caprolactone)‐poly(ethylene glycol)‐poly(ε‐caprolactone) fibrous mats

Fu, Shaozhi; Meng, Xiao-Hang; Fan, Juan; Yang, Linglin; Wen, Qinglian; Ye, Sujuan; Lin, Sheng; Wang, Biqiong; Chen, Lanlan; Wu, Jingbo; Chen, Yue; Fan, Junming; Li, Zhi

doi:10.1002/jbm.b.33032

Cited by 83 publications

(57 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[15][16][17][18] Numerous studies have proposed electrospun meshes loaded with curcumin, given the potential for health care applications. A wide range of polymers has been used, including polycaprolactone (PCL), [19][20][21] polylactic acid (PLA), 22 polylactic-co-glycolic acid, 23 polyvinylpyrrolidone (PVP), 24 silk, 25 and cellulose acetate. 26 Because of the hydrophobic nature of curcumin, the use of hydrophobic polymers such as degradable polyesters is particularly attractive to achieve a slow and prolonged release.…”

Section: Introductionmentioning

confidence: 99%

“…Relatively high amounts of curcumin have been incorporated in electrospun materials, as most of the studies carried out propose proportions of curcumin 1% of the polymer weight. [19][20][21][22]25,28 These materials often lead to concentrations near or above the apoptosis threshold of 10 μM. Although several authors claim benefits from such materials to stimulate repair, it is not clear why these amounts have been selected while data from studies carried out with free curcumin suggest that lower concentrations might be more adequate for wound healing applications.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Mouthuy¹,

Škoc²,

Gašparović³

et al. 2017

IJN

View full text Add to dashboard Cite

Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 μM, respectively), which are near or above the known toxicity threshold of curcumin (~10 μM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Mouthuy¹,

Škoc²,

Gašparović³

et al. 2017

IJN

View full text Add to dashboard Cite

show abstract

“…22,23 Polycaprolactone (PCL) is a biocompatible and biodegradable material approved by the US Food and Drug Administration (FDA). 24,25 The electrospun PCL nanofibrous mesh possesses high porosity and good mechanical strength, which are important factors for wound dressings.…”

mentioning

confidence: 99%

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

Liu¹,

Luo²,

Wang³

et al. 2017

IJN

View full text Add to dashboard Cite

Bacterial infection is a major hurdle to wound healing, and the overuse of antibiotics have led to global issue, such as emergence of multidrug-resistant bacteria, even “super bacteria”. On the contrary, nanosilver (NS) can kill bacteria without causing resistant bacterial strains. In this study, NS was simply generated in situ on the polycaprolactone (PCL) nanofibrous mesh using an environmentally benign and mussel-inspired dopamine (DA). Scanning electron microscopy showed that NS uniformly formed on the nanofibers of PCL mesh. Fourier transform infrared spectroscopy revealed the step-by-step preparation of pristine PCL mesh, including DA coating and NS formation, which were further verified by water contact angle changing from hydrophobic to hydrophilic. To optimize the NS dose, the antibacterial activity of PCL/NS against Staphylococcus aureus , Escherichia coli and Acinetobacter baumannii was detected by bacterial suspension assay, and the cytotoxicity of NS was evaluated using cellular morphology observation and Cell Counting Kit-8 (CCK8) assay. Then, inductively coupled plasma atomic emission spectrometry exhibited that the optimized PCL/NS had a safe and sustained silver release. Moreover, PCL/NS could effectively inhibit bacterial infection in an infectious murine full-thickness skin wound model. As demonstrated by the enhanced level of proliferating cell nuclear antigen (PCNA) in keratinocytes and longer length of neo-formed epidermis, PCL/NS accelerated wound healing by promoting re-epithelialization via enhancing keratinocyte proliferation in infectious wounds.

show abstract

“…We have demonstrated that the electrospun nanofibrous dressings presented in this study have the capacity to be a biocompatible, safe, and effective alternative for wound healing and dressing of choice concurring with other in vivo animal studies. [15][16][17] Electrospun nanofibers properties used for wound management including hydrophilicity, flexibility and strength, biocompatibility, and specific cell interactions are largely determined by the chemical composition of the materials used. 25 Formulation A was biodegradable polyester based requiring only 1 minute for creation of a dressing in a desirable and workable thickness and texture in comparison with 1.5 min for formulations B and C and 2 min for formulation D. Moreover, in formulations B and C the smell of the solvents was evident in the first minute of device operation, while in formulation A and D it was not noticeable, which is an important element in occupational health and safety for any associated risks from exposure to these materials.…”

Section: Discussionmentioning

confidence: 99%

“…15 Subsequently, the use of electrospun biopolymer nanofibrous dressings allows a biocompatible, simple, and cost-effective alternative to wound management and has gained considerable interest to wound care clinicians. 11,16,17 Electrospun polymer nanofibrous dressings have shown to facilitate cell migration and proliferation of the wound bed, provide hemostasis, gaseous exchange, and management of wound exudate.…”

Section: Introductionmentioning

confidence: 99%

The Feasibility of a Handheld Electrospinning Device for the Application of Nanofibrous Wound Dressings

Haik

Kornhaber

Blal

et al. 2017

Advances in Wound Care

View full text Add to dashboard Cite

{These authors contributed equally to this work. Objectives:The aim of this study was to determine the feasibility of a portable electrospinning device for the application of wound dressings. Approach: Four polymer nanofibers dressings were applied on superficial partial thickness wounds to a porcine model and compared with a traditional paraffin tulle gras dressing. The polymer nanofibrous dressings were applied using a handheld portable electrospinning device activated at a short distance from the wound. The partial thickness donor sites were evaluated on day 2, 7, and 14 when dressings were removed and tissue samples were taken for histological examination. Results: No significant difference was detected between the different electrospun nanofibrous dressings and traditional paraffin tulle gras. Desirable characteristics of the electrospun nanofiber dressing group included nontouch technique, ease of application, adherence and reduction in wound edema and inflammation. There was no delayed wound healing or signs of infection reported in both the electrospun nanofiber and traditional tulle gras dressings. Innovation: Used on partial thickness wounds, polymer electrospun nanofiber dressings provide excellent surface topography and are a nontouch, feasible, and safe method to promote wound healing with the potential to reduce wound infections. Such custom-made nanofibrous dressings have implications for the reduction of pain and trauma, number of dressing changes, scarring, and an added cost benefit. Conclusion: We have demonstrated that this portable handheld electrospinning device can be utilized for different formulations and materials and customized according to the characteristics of the target wound at the various stages of wound healing.

show abstract

Acceleration of dermal wound healing by using electrospun curcumin‐loaded poly(ε‐caprolactone)‐poly(ethylene glycol)‐poly(ε‐caprolactone) fibrous mats

Cited by 83 publications

References 38 publications

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Investigating the use of curcumin-loaded electrospun filaments for soft tissue repair applications

Optimization and integration of nanosilver on polycaprolactone nanofibrous mesh for bacterial inhibition and wound healing in vitro and in vivo

The Feasibility of a Handheld Electrospinning Device for the Application of Nanofibrous Wound Dressings

Contact Info

Product

Resources

About