Accumulation of phytoalexins in leaves of plane tree (Platanus spp.) expressing susceptibility or resistance toCeratocystis fimbriata f. sp.platani

Modafar, Cherkaoui El; Clérivet, Alain; Vigouroux, A.; Macheix, Jean‐Jacques

doi:10.1007/bf01874474

Cited by 32 publications

(16 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among these are the coumarins, which feature in the defence response against pathogen attack and abiotic stress. Scopolin is produced along with its related aglycon scopoletin (the active form) in leaves as part of the host's rapid response to both pathogen attack (Sequeira, ; Spring et al ., ; Elmodafar et al ., ; Garcia et al ., ), and abiotic stress (Koeppe et al ., ). Its role in leaves is thought to combine antibiosis (resulting from the toxicity of the phenolic moiety) with reactive oxygen species (ROS) scavenging.…”

Section: Introductionmentioning

confidence: 98%

Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex

et al. 2018

View full text Add to dashboard Cite

Secondary metabolites are involved in the plant stress response. Among these are scopolin and its active form scopoletin, which are coumarin derivatives associated with reactive oxygen species scavenging and pathogen defence. Here we show that scopolin accumulation can be induced in the root by osmotic stress and in the leaf by low-temperature stress in Arabidopsis thaliana. A genetic screen for altered scopolin levels in A. thaliana revealed a mutant compromised in scopolin accumulation in response to stress; the lesion was present in a homologue of THO1 coding for a subunit of the THO/TREX complex. The THO/TREX complex contributes to RNA silencing, supposedly by trafficking precursors of small RNAs. Mutants defective in THO, AGO1, SDS3 and RDR6 were impaired with respect to scopolin accumulation in response to stress, suggesting a mechanism based on RNA silencing such as the trans-acting small interfering RNA pathway, which requires THO/TREX function.

show abstract

Section: Introductionmentioning

confidence: 98%

Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Known post-infection host defense mechanisms involve physical factors such as the occlusion of the xylematic vessels and the compartmentalization of infected tissue areas as well as the production of flavans, umbelliferone, and scopoletine phytoalexins (6 -9). Unfortunately, only resistant P. occidentalis clones, not yet acclimatized to Europe, localized the disease (7,8). Recent papers (10,11) have shown that C. fimbriata f. sp.…”

mentioning

confidence: 99%

Purification, Characterization, and Amino Acid Sequence of Cerato-platanin, a New Phytotoxic Protein from Ceratocystis fimbriata f. sp. platani

Pazzagli¹,

Cappugi²,

Manao³

et al. 1999

Journal of Biological Chemistry

169

173

View full text Add to dashboard Cite

A new phytotoxic protein (cerato-platanin) of about 12.4 kDa has been identified in culture filtrates of the Ascomycete Ceratocystis fimbriata f. sp. platani, the causal agent of canker stain disease. The toxicity of the pure protein was bioassayed by detecting the inducing necrosis in tobacco leaves. The pure protein also elicited host synthesis of fluorescent substances in tobacco and plane (Platanus acerifolia) leaves. We purified the protein from culture medium to homogeneity. Its complete amino acid sequence was determined; this protein consists of 120 amino acid residues, contains 4 cysteines (S-S-bridged), and has a high percentage of hydrophobic residues. The molecular weight calculated from the amino acid sequence agrees with that determined by mass spectrometry, suggesting that no post-transnational modification occurs. Searches performed by the BLAST program in data banks (Swiss-Prot, EBI, and GenBank) revealed that this protein is highly homologous with two proteins produced by other Ascomycete fungi. One, produced during infection of wheat leaves, is codified by the snodprot1 gene of Phaeosphaeria nodorum (the causal agent of glume blotch of wheat), whereas the other is the rAsp f13 allergen from Aspergillus fumigatus. Furthermore, the N terminus of cerato-platanin is homologous with that of cerato-ulmin, a phytotoxic protein belonging to the hydrophobin family and produced by Ophiostoma (Ceratocystis) ulmi, a fungus responsible for Dutch elm disease.The European plane tree (Platanus acerifolia) is an ornamental plant species of the urban environment. A great number of plane trees in the parks and towns of southern Europe have been destroyed by Ceratocystis fimbriata (Ell. and Halst.) Davidson f. sp. platani Walter, the Ascomycete responsible for canker stain disease (1). This disease is characterized by foliar wilting and spreading lesions that involve phloem, cambium, and extensive regions of sapwood (2, 3). The pathogen spreads from tree to tree by means of root grafts of closely spaced plants and, more frequently, through wounds caused by pruning (4).The American species Platanus occidentalis has been shown to contain a source of resistance to C. fimbriata f. sp. platani that could prove of great interest in the genetic improvement of the European plane (5). Known post-infection host defense mechanisms involve physical factors such as the occlusion of the xylematic vessels and the compartmentalization of infected tissue areas as well as the production of flavans, umbelliferone, and scopoletine phytoalexins (6 -9). Unfortunately, only resistant P. occidentalis clones, not yet acclimatized to Europe, localized the disease (7,8). Recent papers (10, 11) have shown that C. fimbriata f. sp. platani displays an array of phytotoxic metabolites possibly involved in determining some of the symptoms of canker stain.In the present paper we report on the purification procedure, the amino acid sequence, and the characterization of the biological activity of a new protein (named cerato-platanin) from the cul...

show abstract

“…The derivatives of oleuropein, like oleuropein glucoside, seems to be implied in the defence of the olive tree by inhibiting the pectinases of F. oleagineum (Graniti 1993) and by constituting a precursor of phytoalexins (Uccella 2000), extremely toxic molecules induced in response to pathogen infection (El Modafar et al 1995. The derivative nonglucosidic form (oleuropein aglycone) of oleuropein expresses antioxidant activity (Galli and Visioli 1999) and great cytotoxicity (Babich and Visioli 2003).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of susceptibility of an olive progeny (Picholine x Meski) to olive leaf spot disease caused by Fusicladium oleagineum

Rhouma¹,

Chettaoui²,

Krid³

et al. 2012

Eur J Plant Pathol

View full text Add to dashboard Cite

show abstract

Accumulation of phytoalexins in leaves of plane tree (Platanus spp.) expressing susceptibility or resistance toCeratocystis fimbriata f. sp.platani

Cited by 32 publications

References 25 publications

Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex

Accumulation of the coumarin scopolin under abiotic stress conditions is mediated by the Arabidopsis thaliana THO/TREX complex

Purification, Characterization, and Amino Acid Sequence of Cerato-platanin, a New Phytotoxic Protein from Ceratocystis fimbriata f. sp. platani

Evaluation of susceptibility of an olive progeny (Picholine x Meski) to olive leaf spot disease caused by Fusicladium oleagineum

Contact Info

Product

Resources

About