Accumulation of Phytohormones by Soil Bacteria Azotobacter Vinelandii and Bacillus Subtilis Under the Influence of Nanomaterials

Abstract

Azotobacter vinelandii IMV B-7076 was shown to be capable of biosynthesis and accumulation of various biologically active substances, including high amounts of 3-indoleacetic acid (IAA) in free and bound forms, in the cultural medium. Cultivation of these bacteria with vermiculite particles had increased accumulation of abscisic acid (ABA) in 2.3 times compared to the control. Addition of silica dioxide nanoparticles to the nutrient medium had resulted in higher cytokinin content. Thus, the detected amount of zeatin was 2.4 times higher; zeatin-riboside – higher in 3.6 times; while the content of zeatin-glucoside had a slight increase by 3%. High amounts of cytokinins, 3-indoleacetic and abscisic acids were also detected in the cultural medium of Bacillus subtilis IMV B-7023. Bacteria cultivation in the presence of vermiculite particles in nutrient medium had stimulated an increase of isopentyl adenine content in 4.0 times compared to control. Substantial (in 4.5 times) increase of 3-indoleacetic acid synthesis compared to control was detected. Addition of nanoparticles to the nutrient medium of Bacillus subtilis IMV B-7023 had also promoted synthesis of the abscisic acid in 1.7 times compared to control.

Natural and Synthetic Nanomaterials in Microbial Biotechnologies for Crop Production

“…It was found, that during the cultivation of bacilli with 5 g/L of this minerals the content of zeatin in the culture increased by 17 %, that of zeatin riboside -by 20 %, and zeatin glucoside -by 144 % [69]. These nanoparticles had a significant stimulating effect on the synthesis of phytohormones by the bacteria A. vinelandii IMV B-7076 [74].…”

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“…Researchers reported the ability of Azotobacter to produce phytohormones Indole Acetic Acid (IAA) and Cytokinines (CKs) in liquid culture (Viscardi et al, 2016;Chobotarov et al, 2017;Hindersah et al, 2020). The CKs include zeatin, zeatin-riboside dan zeatin glucose also detected in nanoparticle solid-based inoculant (Chobotarov et al, 2017).…”

Effect of Nanoparticles of Natural Minerals, Iron and Mangan Compounds, on the Growth and Superoxide Dismutase Activity of Bacillus Subtilis Imv B-7023

“…72856) with particles of mineral clay bentonite was used to develop a highly efficient complex bacterial preparation Azogran for agriculture. It is components are characterized by the capability of synthesizing a wide range of biologically active substances (Ocheretyanko et al 2016;Chobotarov et al 2017), inhibiting the diffusion of phytopathogens and phytophages in the phytosphere (Roi et al 2005;Roy et al 2014), and causing antioxidant impact on the plant seeds, exposed to hydrogen peroxide (Skorochod et al 2011;Skorochod et al 2013). The metabolic processes in living cells including microorganisms are accompanied by the formation of reactive oxygen intermediates (ROI), excessive amounts of which may lead to oxidative stress and damage to biomolecules.…”