Azotobacter vinelandii IMV B-7076 was shown to be capable of biosynthesis and accumulation of various biologically active substances, including high amounts of 3-indoleacetic acid (IAA) in free and bound forms, in the cultural medium. Cultivation of these bacteria with vermiculite particles had increased accumulation of abscisic acid (ABA) in 2.3 times compared to the control. Addition of silica dioxide nanoparticles to the nutrient medium had resulted in higher cytokinin content. Thus, the detected amount of zeatin was 2.4 times higher; zeatin-riboside – higher in 3.6 times; while the content of zeatin-glucoside had a slight increase by 3%. High amounts of cytokinins, 3-indoleacetic and abscisic acids were also detected in the cultural medium of Bacillus subtilis IMV B-7023. Bacteria cultivation in the presence of vermiculite particles in nutrient medium had stimulated an increase of isopentyl adenine content in 4.0 times compared to control. Substantial (in 4.5 times) increase of 3-indoleacetic acid synthesis compared to control was detected. Addition of nanoparticles to the nutrient medium of Bacillus subtilis IMV B-7023 had also promoted synthesis of the abscisic acid in 1.7 times compared to control.

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The present study investigated the influence of different nanomaterials on physiological and biochemical activity of the nitrogen-fixing bacteria Azotobacter vinelandii IMV V-7076 and phosphate mobilizing bacteria Bacillus subtilis IMV V-7023 for the development of high-efficient free-flowing bacterial complex for crop production. Among the studied nanomaterials, vermiculite stimulated the most effectively bacterial growth, synthesis of amino acids and phytohormones, dehydrogenase, catalase and peroxidase activities. Based on vermiculite and highly efficient strains of bacteria Azotobacter vinelandii IMV V-7076 and Bacillus subtilis IMV V-7023, a free-flowing bacterial complex preparation for crop production was created. The preparation was stable during storage, it improved the nitrogenous and phosphorus nutrition of plants stimulated their growth by biologically active substances and protected plants from lesion by phytopathogenic micromicetes and bacteria.

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