Accuracy of the diagnostic tests for the detection of Chagas disease: a systematic review and meta-analysis

Candia-Puma, Mayron Antonio; Machaca-Luque, Laura Yesenia; Roque-Pumahuanca, Brychs Milagros; Galdino, Alexsandro Sobreira; Giunchetti, Rodolfo Cordeiro; Coelho, Eduardo Antônio Ferraz; Chávez-Fumagalli, Miguel Á.

doi:10.37766/inplasy2022.9.0132

2022

DOI: 10.37766/inplasy2022.9.0132

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Accuracy of the diagnostic tests for the detection of Chagas disease: a systematic review and meta-analysis

Mayron Antonio Candia-Puma¹,

Laura Yesenia Machaca-Luque²,

Brychs Milagros Roque-Pumahuanca³

et al.

Abstract: Review question / Objective: The objective of the current work is to systematically review and summarize the available literature on the diagnostic accuracy of diagnostic tests for Chagas Disease. Eligibility criteria: The studies were selected in three stages. In the first, non-English language articles, duplicate articles, reviews, and meta-analyses were excluded, only articles published after 1990 and conducted on humans were included. In the second stage, the titles and ab-stracts of the articles selected … Show more

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A Systematic Review and Meta-Analysis Comparing the Diagnostic Accuracy Tests of COVID-19

et al. 2023

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In this paper, we present a systematic review and meta-analysis that aims to evaluate the reliability of coronavirus disease diagnostic tests in 2019 (COVID-19). This article seeks to describe the scientific discoveries made because of diagnostic tests conducted in recent years during the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Between 2020 and 2021, searches for published papers on the COVID-19 diagnostic were made in the PubMed database. Ninety-nine scientific articles that satisfied the requirements were analyzed and included in the meta-analysis, and the specificity and sensitivity of the diagnostic accuracy were assessed. When compared to serological tests such as the enzyme-linked immunosorbent assay (ELISA), chemiluminescence immunoassay (CLIA), lateral flow immunoassay (LFIA), and chemiluminescent microparticle immunoassay (CMIA), molecular tests such as reverse transcription polymerase chain reaction (RT-PCR), reverse transcription loop-mediated isothermal amplification (RT-LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR) performed better in terms of sensitivity and specificity. Additionally, the area under the curve restricted to the false-positive rates (AUCFPR) of 0.984 obtained by the antiviral neutralization bioassay (ANB) diagnostic test revealed significant potential for the identification of COVID-19. It has been established that the various diagnostic tests have been effectively adapted for the detection of SARS-CoV-2; nevertheless, their performance still must be enhanced to contain potential COVID-19 outbreaks, which will also help contain potential infectious agent outbreaks in the future.

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A Systematic Review and Meta-Analysis Comparing the Diagnostic Accuracy Tests of COVID-19

et al. 2023

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Parasite Burden of Trypanosoma cruzi in Whole Blood and Buffy Coat Determined by Real-Time PCR in Individuals with Chronic Chagas Disease

Liempi,

Zulantay,

Varela

et al. 2024

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The objective of this study was to compare, by qPCR, the circulating blood parasite load of Trypanosoma cruzi in the buffy coat, and in whole blood mixed with boiled and unboiled guanidine hydrochloride-EDTA buffer, of individuals with chronic ChD. The concentration and purity of DNA were evaluated in a Nanodrop Denovix DS-11FX Series Spectrophotometer (DeNovix Inc., Wilmington, NC, USA). The parasite load was determined with the Taqman® qPCR system using a Stratagene Mx3000P thermocycler (Agilent Technologies, Santa Clara, CA, USA) with Cruzi 1 and Cruzi 2 satellite primers. Student’s t-test with Bonferroni correction, Chi-squared (χ2) tests and Spearman’s correlation coefficient were applied. The concentration and purity of DNA were higher in the buffy coat. Parasite DNA was detected and quantifiable in the three types of samples in seven patients, without statistically significant differences in the parasite load obtained. Higher correlations were found between the total DNA concentrations and the parasite loads obtained in the samples of the buffy coat.

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Cytokine Networks as Targets for Preventing and Controlling Chagas Heart Disease

et al. 2023

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Chagas disease, a neglected disease caused by the protozoan Trypanosoma cruzi, is endemic in 21 Latin American countries, affecting 6–8 million people. Increasing numbers of Chagas disease cases have also been reported in non-endemic countries due to migration, contamination via blood transfusions or organ transplantation, characterizing Chagas as an emerging disease in such regions. While most individuals in the chronic phase of Chagas disease remain in an asymptomatic clinical form named indeterminate, approximately 30% of the patients develop a cardiomyopathy that is amongst the deadliest cardiopathies known. The clinical distinctions between the indeterminate and the cardiac clinical forms are associated with different immune responses mediated by innate and adaptive cells. In this review, we present a collection of studies focusing on the human disease, discussing several aspects that demonstrate the association between chemokines, cytokines, and cytotoxic molecules with the distinct clinical outcomes of human infection with Trypanosoma cruzi. In addition, we discuss the role of gene polymorphisms in the transcriptional control of these immunoregulatory molecules. Finally, we discuss the potential application of cytokine expression and gene polymorphisms as markers of susceptibility to developing the severe form of Chagas disease, and as targets for disease control.

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Accuracy of the diagnostic tests for the detection of Chagas disease: a systematic review and meta-analysis

Cited by 5 publications

References 0 publications

A Systematic Review and Meta-Analysis Comparing the Diagnostic Accuracy Tests of COVID-19

A Systematic Review and Meta-Analysis Comparing the Diagnostic Accuracy Tests of COVID-19

Parasite Burden of Trypanosoma cruzi in Whole Blood and Buffy Coat Determined by Real-Time PCR in Individuals with Chronic Chagas Disease

Cytokine Networks as Targets for Preventing and Controlling Chagas Heart Disease

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