Accurate MALDI-TOF mass spectrometry identification of a colistin-resistant Moellerella wisconsensis strain

Leroy, Anne-Gaëlle; Malandain, D.; Duchalais, É.; Meurette, G.; Corvec, Stéphane

doi:10.1016/j.medmal.2016.01.009

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…For instance, the Enterobacteriaceae M. wisconsensis , a facultative anaerobic Gram-negative rod, was the major colonizer of B samples already at 0 days. This species is probably an environmental contaminant, as literature reports a variety of isolation sources, including water, foods, and samples of human origin ( Leroy et al, 2016 ). M. wisconsensis can be involved in the dissemination of antibiotic resistance, and its pathogenic role is debated ( Stock et al, 2003 ; Leroy et al, 2016 ; Ahmad et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiota Survey of Sliced Cooked Ham During the Secondary Shelf Life

et al. 2022

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Sliced cooked ham packaged in a modified atmosphere is a popular ready-to-eat product, subjected to abundant microbial contamination throughout its shelf life that can lead to deterioration of both sensorial properties and safety. In this study, the microbial load and the chemical–physical features of cooked ham of five producers were monitored for a period of 12 days after the opening of the packages (i.e., the secondary shelf life), during which the products were stored in a domestic refrigerator at 5.2 ± 0.6°C. The sensorial properties presented a perceivable decay after 8 days and became unacceptable after 12 days. High-performance liquid chromatography analysis and solid-phase microextraction coupled with gas chromatography profiling of volatile metabolites indicated that lactic acid, ethanol, acetic acid, acetoin, 3-methyl-1-butanol, and 2-3 butanediol were the main metabolites that characterized the evolution of the analyzed cooked ham. The microbiota was monitored by 16S ribosomal RNA gene profiling and culture-dependent techniques. Already at the opening of packages, all the products presented high microbial load, generally dominated by lactic acid bacteria, with evident differences among the products. The increase of lactic acid bacteria somehow protected samples from abundant contamination by other bacteria, concurring with the evolution of more safe products. This role was exerted by numerous Latilactobacillus, Leuconostoc, and Carnobacterium species, among which the most frequently detected were Latilactobacillus sakei, Latilactobacillus sakei carnosum, Leuconostoc mesenteroides, and Carnobacterium divergens. Some products presented more complex communities that encompassed Proteobacteria such as Moellerella wisconsensis, Proteus hauseri, Brochothrix thermosphacta, and less frequently Pseudomonas, Erwinia, and Massilia. Opportunistic pathogenic bacteria such as Escherichia coli and Vibrio sp. were found in small quantities. The yeasts Kazachstania servazzii and Debaryomyces hansenii occurred already at 0 days, whereas various species of Candida (Candida zeylanoides, Candida sake, Candida norvegica, and Candida glaebosa) were abundant only after 12 days. These results indicated that the microbiological contaminants overgrowing during the secondary shelf life did not derive from environmental cross-contamination at the opening of the tray but were already present when the packages were opened, highlighting the phases of production up to the packaging as those crucial in managing the safety risk associated to this product.

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Section: Discussionmentioning

confidence: 99%

Microbiota Survey of Sliced Cooked Ham During the Secondary Shelf Life

et al. 2022

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“…Although it is an uncommon bacterium in the daily clinical practice, there are several case reports in the literature describing its isolation from clinical specimens, such as human stool, bile, blood, bronchial aspirate, and wound swab, and its relationship with clinically overt disease [ 1 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 21 ]. Regarding our report, it represents the fourth case of isolation of M. wisconsensis from blood culture and the first case of its isolation from pigtail end and urine cultures.…”

Section: Discussionmentioning

confidence: 99%

Infections Caused by Moellerella wisconsensis: A Case Report and a Systematic Review of the Literature

et al. 2022

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Background: Moellerella wisconsensis, a member of the family of Enterobacteriaceae, although isolated widely in nature, rarely causes infections in humans. Herein, we report a case of isolation of M. wisconsensis from pigtail end culture, urine culture and blood culture in a 76-year-old patient. Objective: To systematically address all the relevant information regarding M. wisconsensis through literature. Methods: We searched PubMed and Scopus databases up to January 2022 and performed a qualitative synthesis of published articles reporting infection from M. wisconsensis in humans. Results: We identified 25 records on PubMed and 43 additional records on Scopus. After removing duplicates, we examined in detail 15 articles. Ten studies with a total of 17 cases were included in our systematic review. Nine studies described isolated case reports, while 1 study described 8 cases. The origin of the infection was the alimentary tract in 9 cases, gallbladder in 4 cases, peritoneal cavity in 2 cases, respiratory tract in 1 case and hemodialysis catheter insertion site in 1 case. In 3 of the aforementioned cases M. wisconsensis was also isolated in blood cultures. Conclusion: Physicians should be aware that M. wisconsensis can be present in multiple clinical specimens and that the antibiotic resistance profile of the isolates may pose significant challenges.

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“…Despite that almost forty years have passed since the first detection of M. wisconsensis in 1984, its exact distribution and potential pathogenicity remain widely unknown. The bacterium has been isolated from human clinical specimens and has been implicated in cases of gastroenteritis, diarrhea, cholecystitis, bacteremia, peritonitis and urinary tract infections [1][2][3][4][5][6][7]. Additionally, it has been retrieved from both domestic and wild animals, as well as from insects and parasites, and has been identified as the causative agent of animal disease [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

ESBL-Producing Moellerella wisconsensis—The Contribution of Wild Birds in the Dissemination of a Zoonotic Pathogen

Sofia

Γιαννακόπουλος

Papageorgiou

et al. 2022

Animals

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Moellerella wisconsensis is an Enterobacteriaceae with unclarified dispersion and pathogenicity. During an ongoing investigation about antimicrobial resistance in Greece, the occurrence of M. wisconsensis was evaluated among wild birds and humans. A total of 445 wild bird and 2000 human fecal samples were collected and screened for the presence of the organism. Subsequently, all M. wisconsensis strains were phenotypically and molecularly characterized regarding their antimicrobial resistance characteristics. Four M. wisconsensis were isolated from a common pheasant (Phasianus colchicus), two Eurasian magpies (Pica pica) and a great white-fronted goose (Anser albifrons). Among these four strains, the three latter presented resistance to 3rd generation cephalosporins, were phenotypically confirmed to produce ESBLs and were found to harbor blaCTX-M-1. The three ESBL isolates additionally exhibited resistance to tetracyclines, while resistance to aminoglycosides was detected in two of them and to trimethoprim/sulfamethoxazole in one. No Moellerella wisconsensis strains were retrieved from the human samples tested. This is the first report that provides evidence of M. wisconsensis dissemination among wild birds in Greece, describing CTX-M-1 production in multidrug resistant wild birds’ isolates of this bacterial species.

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Accurate MALDI-TOF mass spectrometry identification of a colistin-resistant Moellerella wisconsensis strain

Cited by 6 publications

References 8 publications

Microbiota Survey of Sliced Cooked Ham During the Secondary Shelf Life

Microbiota Survey of Sliced Cooked Ham During the Secondary Shelf Life

Infections Caused by Moellerella wisconsensis: A Case Report and a Systematic Review of the Literature

ESBL-Producing Moellerella wisconsensis—The Contribution of Wild Birds in the Dissemination of a Zoonotic Pathogen

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