Acetone

Sifniades, Stylianos; Levy, Alan B.; Bahl, Hubert

doi:10.1002/14356007.a01_079.pub2

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Acetone (dimethylketone) can be regarded as a partially activated hydrocarbon through its oxo‐group and is commonly used in numerous industrial processes, e.g., as solvent or as precursor for chemical syntheses. Furthermore, acetone is also produced by anaerobic, solventogenic bacteria like several Clostridia species (Dürre et al ., ; Sifniades et al ., ). Therefore, it occurs as an important pollutant in waste water and also in natural habitats.…”

Section: Introductionmentioning

confidence: 97%

Two enzymes of the acetone degradation pathway of Desulfococcus biacutus: coenzyme B₁₂‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

Frey

Schneider

Huhn

et al. 2018

Environ Microbiol Rep

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Degradation of acetone by the sulfate-reducing bacterium Desulfococcus biacutus involves an acetone-activation reaction different from that used by aerobic or nitrate-reducing bacteria, because the small energy budget of sulfate-reducing bacteria does not allow for major expenditures into ATP-consuming carboxylation reactions. In the present study, an inducible coenzyme B -dependent conversion of 2-hydroxyisobutyryl-CoA to 3-hydroxybutyryl-CoA was demonstrated in cell-free extracts of acetone-grown D. biacutus cells, together with a NAD -dependent oxidation of 3-hydroxybutyryl-CoA to acetoacetyl-CoA. Genes encoding two mutase subunits and a dehydrogenase, which were found previously to be strongly induced during growth with acetone, were heterologously expressed in E. coli. The activities of the purified recombinant proteins matched with the inducible activities observed in cell-free extracts of acetone-grown D. biacutus: proteins (IMG locus tags) DebiaDRAFT_04573 and 04574 constituted a B -dependent 2-hydroxyisobutyryl-CoA/3-hydroxybutyryl-CoA mutase, and DebiaDRAFT_04571 was a 3-hydroxybutyryl-CoA dehydrogenase. Hence, these enzymes play key roles in the degradation of acetone and define an involvement of CoA esters in the pathway. Further, the involvement of 2-hydroxyisobutyryl-CoA strongly indicates that the carbonyl-C of acetone is added, most likely, to formyl-CoA through a TDP-dependent enzyme that is co-induced in acetone-grown cells and is encoded in the same gene cluster as the identified mutase and dehydrogenase.

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Section: Introductionmentioning

confidence: 97%

Two enzymes of the acetone degradation pathway of Desulfococcus biacutus: coenzyme B₁₂‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

Frey

Schneider

Huhn

et al. 2018

Environ Microbiol Rep

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Selective bio-oxidation of propane to acetone using methane-oxidizing Methylomonas sp. DH-1

Hur

Nguyen

Kim

et al. 2017

Journal of Industrial Microbiology and Biotechnology

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Propane is the major component of liquefied petroleum gas (LPG). Nowadays, the use of LPG is decreasing, and thus utilization of propane as a chemical feedstock is in need of development. An efficient biological conversion of propane to acetone using a methanotrophic whole cell as the biocatalyst was proposed and investigated. A bio-oxidation pathway of propane to acetone in Methylomonas sp. DH-1 was analyzed by gene expression profiling via RNA sequencing. Propane was oxidized to 2-propanol by particulate methane monooxygenase and subsequently to acetone by methanol dehydrogenases. Methylomonas sp. DH-1 was deficient in acetone-converting enzymes and thus accumulated acetone in the absence of any enzyme inhibition. The maximum accumulation, average productivity and specific productivity of acetone were 16.62 mM, 0.678 mM/h and 0.141 mmol/g cell/h, respectively, under the optimized conditions. Our study demonstrates a novel method for the bioconversion of propane to acetone using methanotrophs under mild reaction condition.

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Synthesis of ZnO-CoO/Al2O3 nanoparticles and its application as a catalyst in ethanol conversion to acetone

Mohammed¹,

Ali

Alalwan

et al. 2021

Results in Chemistry

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Acetone

Cited by 3 publications

References 29 publications

Two enzymes of the acetone degradation pathway of Desulfococcus biacutus: coenzyme B₁₂‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

Two enzymes of the acetone degradation pathway of Desulfococcus biacutus: coenzyme B₁₂‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

Selective bio-oxidation of propane to acetone using methane-oxidizing Methylomonas sp. DH-1

Synthesis of ZnO-CoO/Al2O3 nanoparticles and its application as a catalyst in ethanol conversion to acetone

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Acetone

Cited by 3 publications

References 29 publications

Two enzymes of the acetone degradation pathway of Desulfococcus biacutus: coenzyme B12‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

Two enzymes of the acetone degradation pathway of Desulfococcus biacutus: coenzyme B12‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

Selective bio-oxidation of propane to acetone using methane-oxidizing Methylomonas sp. DH-1

Synthesis of ZnO-CoO/Al2O3 nanoparticles and its application as a catalyst in ethanol conversion to acetone

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Two enzymes of the acetone degradation pathway of Desulfococcus biacutus: coenzyme B₁₂‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase

Two enzymes of the acetone degradation pathway of Desulfococcus biacutus: coenzyme B₁₂‐dependent 2‐hydroxyisobutyryl‐CoA mutase and 3‐hydroxybutyryl‐CoA dehydrogenase