Acetylated tubulin is found in all microtubule arrays of two species of pine

Gilmer, Susan; Clay, P.; MacRae, Thomas H.; Fowke, Larry C.

doi:10.1007/bf01282998

Cited by 30 publications

(37 citation statements)

References 41 publications

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“…A strong labeling in the cell cortex typical of the plasma-membrane-associated ER could also be observed (Quader et al 1987). During preprophase/ prophase an Mt-PPB, similar to that described in other Pinus species (Fowke 1993;Gilmer et al 1999), was formed (Fig. 1b).…”

Section: Resultssupporting

confidence: 73%

Endoplasmic reticulum preprophase band in dividing root-tip cells of Pinus brutia

Zachariadis

Quader

Galatis

et al. 2001

Planta

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In dividing root-tip cells of Pinus brutia Ten., immunolocalization of the luminal endoplasmic reticulum (ER) proteins, which have the C-terminal HDEL sequence, reveals that the ER is reorganized during the preprophase/prophase stage. Portions of ER were arrayed into a ring-like structure at the site of the microtubule preprophase band (Mt-PPB). This preprophase ER band (ER-PPB) resembles that of the Mt-PPB. The former undergoes a maturation process closely similar to that of the latter. Our data show that the PPB region has a more complex organization than is currently believed. The probable function(s) of the ER-PPB is discussed.

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Section: Resultssupporting

confidence: 73%

Endoplasmic reticulum preprophase band in dividing root-tip cells of Pinus brutia

Zachariadis

Quader

Galatis

et al. 2001

Planta

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“…Later, substantial acetylated a-tubulin was detected in suspension cells of tobacco (Smertenko et al 1997) and maize (Huang and Lloyd 1999), and its accumulation was stimulated by gibberellic acid (Huang and Lloyd 1999). Interestingly, acetylated a-tubulin was found in root tip cells of conifers but not in root tip cells of angiosperms (Gilmer et al 1999b). Additionally, although the composition of a-tubulin isoforms from maize pollen, anthers and roots reported here shared high similarities in 2-D patterns with that from maize suspension cells (Huang and Lloyd 1999), the extent of acetylation of a-tubulin isoforms differed remarkably, and moreover, acetylated a-tubulin isoforms in maize leaves have more basic pIs than those in cultured cells (Huang and Lloyd 1999).…”

Section: Ptms Of A-tubulinmentioning

confidence: 99%

“…tyrosination (Del Casino et al 1993;Duckett and Lloyd 1994;Smertenko et al 1997;Gilmer et al 1999a), acetylation (Å stro¨m 1992;Smertenko et al 1997;Huang and Lloyd 1999;Gilmer et al 1999b), detyrosination (Duckett and Lloyd 1994;Smertenko et al 1997), and polyglutamylation (Smertenko et al 1997). Moreover, controversial data exist on the presence of acetylated (Å stro¨m 1992;Del Casino et al 1993;Smertenko et al 1997;Gilmer et al 1999b) and detyrosinated (Duckett and Lloyd 1994;Smertenko et al 1997;Gilmer et al 1999a) a-tubulin in plants. In addition, most data available on PTMs of a-tubulin in plants were obtained by immunofluorescence microscopy, which generally focused on one single cell or tissue type, and thereby failed to produce a complete comparative picture of the possible PTMs of each atubulin isoform in different tissues of the same plant species.…”

Section: Introductionmentioning

confidence: 99%

Post-translational modifications of ?-tubulin in Zea mays L. are highly tissue specific

Wang

Vignani

Scali

et al. 2004

Planta

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To further understand post-translational modifications (PTMs) of plant alpha-tubulin, post-translationally modified alpha-tubulin isoforms from selected tissues of Zea mays L. were examined using two-dimensional electrophoresis and immunoblotting. Except for polyglycylated tubulin, tyrosinated, detyrosinated, acetylated and polyglutamylated alpha-tubulin isoforms were all present in maize tissues. Tyrosinated alpha-tubulin was the predominant variant in all cases, with isoforms alpha1-alpha4 (alpha5) being the most common components. Leaves exhibited a striking difference in PTM patterns of alpha-tubulin isoforms compared to other tissues examined. In leaves, several major specific isoforms were highly modified by detyrosination, acetylation and polyglutamylation. In pollen and anthers, only the most abundant isoform alpha3 was acetylated to an appreciable extent, and no acetylated isoform was found in roots. Similarly, in pollen, anthers and roots, only alpha3 was appreciably polyglutamylated. Additionally, a detyrosinated isoform alpha6 was present in anthers and in leaves, while the tyrosinated isoform alpha6 seemed to be pollen specific. These results indicate that certain types of PTM of plant alpha-tubulin preferentially occur in a tissue-specific way.

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“…Physiologically much stronger immunoreactivity of antibodies against acetylated tubulin was recorded in MT arrays of pines (Pinus radiata, Pinus contorta) than of angiosperms (A. cepa, Helianthus annuus), where no acetylated tubulin was detected (Gilmer et al 1999). In conifers, MT arrays showing a high degree of acetylation corresponded to relatively stable MTs, while those of lower acetylation reflected the most dynamic MT networks such as the perinuclear MTs in prophase and telophase cells (Gilmer et al 1999). These results have correlated tubulin acetylation with a higher degree of MT stabilization.…”

Section: Universality Of Microtubule Disruption By Cr(vi)mentioning

confidence: 99%

Hexavalent chromium-induced differential disruption of cortical microtubules in some Fabaceae species is correlated with acetylation of α-tubulin

2015

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The effects of hexavalent chromium [Cr(VI)] on the cortical microtubules (MTs) of five species of the Fabaceae family (Vicia faba, Pisum sativum, Vigna sinensis, Vigna angularis, and Medicago sativa) were investigated by confocal laser scanning microscopy after immunolocalization of total tubulin with conventional immunofluorescence techniques and of acetylated α-tubulin with the specific 6-11B-1 monoclonal antibody. Moreover, total α-tubulin and acetylated α-tubulin were quantified by Western immunoblotting and scanning densitometry. Results showed the universality of Cr(VI) detrimental effects to cortical MTs, which proved to be a sensitive and reliable subcellular marker for monitoring Cr(VI) toxicity in plant cells. However, a species-specific response was recorded, and a correlation of MT disturbance with the acetylation status of α-tubulin was demonstrated. In V. faba, MTs were depolymerized at the gain of cytoplasmic tubulin background and displayed low α-tubulin acetylation, while in P. sativum, V. sinensis, V. angularis, and M. sativa, MTs became bundled and changed orientation from perpendicular to oblique or longitudinal. Bundled MTs were highly acetylated as determined by both immunofluorescence and Western immunoblotting. Tubulin acetylation in P. sativum and M. sativa preceded MT bundling; in V. sinensis it followed MT derangement, while in V. angularis the two phenomena coincided. Total α-tubulin remained constant in all treatments. Should acetylation be an indicator of MT stabilization, it is deduced that bundled MTs became stabilized, lost their dynamic properties, and were rendered inactive. Results of this report allow the conclusion that Cr(VI) toxicity disrupts MTs and deranges the MT-mediated functions either by depolymerizing or stabilizing them.

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Acetylated tubulin is found in all microtubule arrays of two species of pine

Cited by 30 publications

References 41 publications

Endoplasmic reticulum preprophase band in dividing root-tip cells of Pinus brutia

Endoplasmic reticulum preprophase band in dividing root-tip cells of Pinus brutia

Post-translational modifications of ?-tubulin in Zea mays L. are highly tissue specific

Hexavalent chromium-induced differential disruption of cortical microtubules in some Fabaceae species is correlated with acetylation of α-tubulin

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