Acetylcholinesterase-based biosensor electrodes for organophosphate pesticide detection

Vakurov, Alexander; Simpson, Clinton; Daly, Catherine; Gibson, Timothy D.; Millner, Paul A.

doi:10.1016/j.bios.2004.03.039

Cited by 47 publications

(42 citation statements)

References 14 publications

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“…A similar strategy was employed by Yang et al [108] with the only difference being trans-4-cinnamic acid diazonium tetrafluoroborate was used to modify the electrode. Other workers have modified electrodes with 4-nitrophenyl diazonium salt followed by reductive conversion of the nitro group to an amine followed by attachment of the enzymes, glucose oxidase [71], acetylcholine esterase for the detection of pesticides by enzyme inhibition [109] or tyrosinase [77] for detecting phenols at diamond electrodes. More complicated strategies for enzyme and other proteins attachment to electrodes modified with aryl diazonium salts have also been developed.…”

Section: Aryl Diazonium Salt Modified Carbon Electrodesmentioning

confidence: 99%

Advances in Interfacial Design for Electrochemical Biosensors and Sensors: Aryl Diazonium Salts for Modifying Carbon and Metal Electrodes

2008

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A high degree of control over the modification of electrode surfaces is required for many sensing applications. This critical review briefly outlines some of the considerations for interfacial design in amperometric sensors and discusses some of advantages and disadvantages of alkanethiol modified metal electrodes for such applications before concentrating on the modification of electrodes surfaces using aryl diazonium salts. The pros and cons of this chemistry, and the application of aryl diazonium salts for sensing on carbon electrodes is reviewed before recent advances in using this chemistry for modifying metal electrodes are presented.

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Section: Aryl Diazonium Salt Modified Carbon Electrodesmentioning

confidence: 99%

Advances in Interfacial Design for Electrochemical Biosensors and Sensors: Aryl Diazonium Salts for Modifying Carbon and Metal Electrodes

2008

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“…The enzyme non-covalently immobilized onto polyethyleneimine modified screen-printed carbon electrodes showed an improvement of stability when compared to non-immobilized AChE, AChE covalently immobilized onto dialdehyde and polyethyleneimine modified electrodes. Several stabilizer mixtures were also employed for an additional stabilization of AChE, demonstrating higher storage stability in the dry state with dextran-sulphate/sucrose or polygalacturonic acid/sucrose mixtures [138].…”

Section: Immobilisationmentioning

confidence: 99%

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

et al. 2010

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The present review reports the research carried out during last 9 years on biosensors based on cholinesterase inhibition for nerve agents, organophosphorus and carbammic insecticides, and aflatoxin B 1 detection. Relative applications in environmental and food areas are also reported. Special attention is paid to the optimization of parameters such as enzyme immobilization, substrate concentration, and incubation time in the case of reversible inhibition by aflatoxin B 1 or irreversible inhibition by organophosphorus and carbamic insecticides, and nerve agents in order to optimize and improve the analytical performances of the biosensor. Evaluation of selectivity of the system is also discussed.

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“…amine group, hydroxyl group and carboxy group, are drawn in by the ring-opening reaction of epoxy groups, the chemical property modification of polymer is obtained and the polymer can be changed into functional polymer with biocompatibility. [12][13][14][15] In this work, with glycidyl methacrylate (GMA) as monomer and ethylene glycol dimethylacrylate (EGDMA) as cross linking agent, cross linking microbeads with an average diameter of 100 μm were synthesized by suspension copolymerization. Subsequently, by the active epoxy groups of GMA, the GMA/EGDMA microbeads were modified with aminating agents of hexanediamine and other three kinds of multiethylene multi-amine.…”

Section: Introductionmentioning

confidence: 99%

Preparation and adsorption property of aminated cross linking microbeads of GMA/EGDMA for bilirubin

2009

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Cross linking microbeads with a controllable diameter were synthesized by suspension copolymerization of the monomer glycidyl methacrylate (GMA) and the cross linking agent ethylene glycol dimethylacrylate (EGDMA). By the ring-opening reaction of the epoxy groups, the microbeads GMA/EGDMA were modified with different aminating agents and resulting in the aminated microbeads. The morphology of the microbeads was characterized by SEM. The adsorption property of aminated microbeads for bilirubin was investigated, and the effects of various factors, such as the chemical structures of the aminating agents, pH values of the medium and the presence of bovine serum albumin in the adsorption medium, on the adsorption property were examined. The experimental results show that the aminated microbeads have strong adsorption ability for bilirubin, and the isotherm adsorption behaviour is fitted to Freundlich equation satisfactorily. The adsorption ability of the aminated microbeads modified with hexanediamine is stronger than that of others, and the longer the molecule of multi-ethylene multiamine, the weaker the adsorption ability for bilirubin. The pH value of the medium affects the adsorption ability greatly, as pH = 6, the adsorption ability is strongest. In the presence of BSA, the microbeads still have a higher adsorption capacity towards bilirubin.

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Acetylcholinesterase-based biosensor electrodes for organophosphate pesticide detection

Cited by 47 publications

References 14 publications

Advances in Interfacial Design for Electrochemical Biosensors and Sensors: Aryl Diazonium Salts for Modifying Carbon and Metal Electrodes

Advances in Interfacial Design for Electrochemical Biosensors and Sensors: Aryl Diazonium Salts for Modifying Carbon and Metal Electrodes

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

Preparation and adsorption property of aminated cross linking microbeads of GMA/EGDMA for bilirubin

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