Acetylcholinesterase Inhibitory and Antioxidant Properties of Thai Vegetables

Langyanai, Supat; Chaniad, Prapaporn; Puripattanavong, Jindaporn

doi:10.18178/ijpmbs.6.2.67-72

Cited by 8 publications

(7 citation statements)

References 20 publications

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“…below 35%. In Bitter gourd, the result was significant which is in accordance with (Langyani, 2017). The found a significant result in Cauliflower and Okra which supports our result (Sahu et al, 2017).…”

Section: Discussionsupporting

confidence: 90%

Organophosphate and Carbamate Pesticides Residue Analysis in Vegetables Produced in Far-West Nepal

Budha¹,

Sapkota²,

Acharya³

et al. 2022

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This is an experimental research study carried out at the laboratory of wholesale fruits and vegetable market, Attarya, Kailali, to analyze organophosphate and carbamate pesticide residue in vegetables grown and marketed in four different districts of far-west Nepal by Rapid Bioassay of Pesticide Residue. The main goal of this study is to determine the proportion of AChE inhibition in various vegetable commodities and to compare the pesticide residue. Seven different vegetables were collected purposively from four distinct areas in Nepal’s far west region. Each of the 28 vegetable samples was analyzed for both organophosphate and carbamate residues. The sample was extracted from each vegetable and examined through a spectrophotometer and the data are interpreted. The experiment’s findings suggest that the Beans from Kanchanpur had the highest OP (21.69 percent) while cucumber from Dadeldhura had the lowest (0.07 percent). The bitter gourd of Kailali had the greatest C (24.39 percent), while the bottle gourd of Doti had the lowest (0.49 percent). All 56 items examined in the Attariya market laboratory were found to have low pesticide residue, with less than 35%. However, the waiting period must be strictly adhered to.

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Section: Discussionsupporting

confidence: 90%

Organophosphate and Carbamate Pesticides Residue Analysis in Vegetables Produced in Far-West Nepal

Budha¹,

Sapkota²,

Acharya³

et al. 2022

Env.cont.Reviews

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“…The inhibition of AChE activity was measured using the spectrophotometric Ellman's method with minor modifications using acetyl-thiocholine (ATCI) as a substrate [33,34]. The acetylcholinesterase enzyme hydrolyses the acetylthiocholine to thiocholine and acetic acid as products.…”

Section: Determination Of Acetylcholinesterase (Ache) Inhibitory Acti...mentioning

confidence: 99%

“…The AChE inhibitory activity of the at-line micro-fractions was also determined using Ellman's colorimetric method with minor modifications [34]. First, 50 μL of Tris-HCl buffer (pH 8.0) was added to the dried micro-fractions and the mixture was shaken for 5 min.…”

Section: Antioxidant Activities and Acetylcholinesterase (Ache) Inhib...mentioning

confidence: 99%

“…Similar findings were reported by previous research works, which indicate that most of active compounds were obtained in crude methanolic extracts of many plants. Thus, the crude methanolic extract is endowed with the greatest antioxidant activity [32,34]. Butylated hydroxytoluene (BHT) was used as the positive control and presented an IC50 value of 10.6 ± 0.1 μg/mL as shown in Table 1.…”

Section: Investigation Of Total Extraction Yield (%) and Antioxidant ...mentioning

confidence: 99%

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Identification of Antioxidants and Acetylcholinesterase Inhibitors from Hapaline benthamiana Schott. Using at Line LC-ESI-QTOF MS/MS

et al. 2022

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In this work, total phenolics, total flavonoids, antioxidant activity, and acetylcholinesterase inhibition potency of Hapaline benthamiana schott are reported. In addition, the investigation of antioxidant and anti-acetylcholinesterase compounds of this plant coupled to the at line-LC-ESI-QTOF-MS/MS technique is introduced for the first time. The results show that the methanolic extract contains the highest amount of total phenolics, total flavonoids, antioxidant activity, and acetylcholinesterase inhibition activity. The at line-LC-ESI-QTOF-MS/MS technique has provided information on the biological activity of each compound while mass spectrometry provided information on their molecular weights. Based on their mass spectra, 53 compounds were identified with most of them being phenolic, flavonoid, and glycolipid compounds. Seventeen compounds exhibited a strong antioxidant activity ranging between 51.52 - 63.58 % inhibition whereas one compound, namely septentriodine, showed a strong acetylcholinesterase inhibitory effect of more than 50 %. This study indicates that Hapaline benthamiana schott is a source of active compounds containing antioxidant and anti-acetylcholinesterase activities with health benefits. In addition, at line-LC-ESI-QTOF-MS/MS has been shown to be an effective technique for the rapid and simultaneous analyses of antioxidant and anti-acetylcholinesterase compounds in plant extracts. HIGHLIGHTS Investigation of antioxidants and acetylcholinesterase inhibitors from Hapaline benthamiana Schott coupled to at line-LC-ESI-QTOF-MS/MS technique is reported for the first time Fifty-three compounds from methanolic extract were successfully identified Eighteen compounds exhibited strong biological activity This technique is a rapid and valuable tool for the identification of lead compounds in complex extracts and for the investigation of their bioactivity GRAPHICAL ABSTRACT

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“…The development of AD is caused by depletion of the acetylcholine neurotransmitter (ACh) due to acetylcholinesterase (AChE) [ 3 , 4 ]. AChE and butylcholinesterase (BChE) hydrolyse ACh within the synaptic cleft [ 5 ]; therefore, one of the possible therapeutic strategies entails the use of AChE [ 6 , 7 ], which, according to the cholinergic theory, results in the elevation of the acetylcholine concentration in the brain and, therefore, improvement of the cognitive function in AD patients [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Honeys as Possible Sources of Cholinesterase Inhibitors

et al. 2022

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Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterised by low levels of the neurotransmitter (acetylcholine), oxidative stress, and inflammation of the central nervous system. The only currently available form of treatment entails the administration of AChE/BChE (acetylcholinesterase/butyrylcholinesterase) inhibitors to patients diagnosed with the disease. However, AD prevention is possible by administering the correct inhibitors with food. The aim of this study was to examine 19 types of honey in terms of their contents of cholinesterase inhibitors. The inhibition of AChE and BChE relative to the respective honey samples was evaluated using Ellman’s colorimetric method, including the “false-positive” effect. The highest potential for AChE inhibition was observed in the case of thyme honey (21.17% inhibition), while goldenrod honey showed the highest capacity for BChE inhibition (33.89%). Our study showed that honeys may provide a rich source of cholinesterase inhibitors and, in this way, play a significant role in AD.

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Acetylcholinesterase Inhibitory and Antioxidant Properties of Thai Vegetables

Cited by 8 publications

References 20 publications

Organophosphate and Carbamate Pesticides Residue Analysis in Vegetables Produced in Far-West Nepal

Organophosphate and Carbamate Pesticides Residue Analysis in Vegetables Produced in Far-West Nepal

Identification of Antioxidants and Acetylcholinesterase Inhibitors from Hapaline benthamiana Schott. Using at Line LC-ESI-QTOF MS/MS

Honeys as Possible Sources of Cholinesterase Inhibitors

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