Acid and bile tolerance of spore-forming lactic acid bacteria

Hyronimus, Bertrand; Marrec, Claire Le; Sassi, A. Hadj; Deschamps, Alain

doi:10.1016/s0168-1605(00)00366-4

Cited by 259 publications

(170 citation statements)

References 12 publications

Supporting

Mentioning

142

Contrasting

Unclassified

Order By: Relevance

“…B. coagulans showed optimum stability at pH 6.8 condition. Study on the spore forming lactic acid bacillus by Hyronimus and coworkers [23] had similar observations for acidic pH environment and found that the spore forming lactic acid bacillus are sensitive to low pH environment. Other lactobacilli like Lactobacillus acidophilus, a common probiotic, was found to be extremely sensitive to low pH environment.…”

Section: Aqueous Ph Stability Profile Of B Coagulansmentioning

confidence: 74%

Physicochemical Properties and Excipient Compatibility Studies of Probiotic Bacillus coagulans Spores

Bora¹,

Puri²,

Bansal³

2009

Sci. Pharm.

View full text Add to dashboard Cite

The probiotic formulations are susceptible to loss in viability due to formulation, processing, storage and in vivo environment. The aim of the present study was to perform preformulation studies of probiotic Bacillus coagulans spores to aid designing of stable formulations. Bacillus coagulans spores were studied for hygroscopicity, resistance to compaction force, aqueous pH stability, and excipient compatibility. The spores were found to be moderately hygroscopic with a significant loss of microbiological assay at water activity value of more than 0.5. Progressive loss of viability from 95% to 58% was observed with increase in compaction force from 1000 to 4000 psi. Aqueous suspension of Bacillus coagulans spores in buffer solutions of pH 1.2 to 8 showed rapid degradation with maximal stablility in pH 6.8. Excipient compatibility studies showed reduced assay with citric acid monohydrate, meglumine and sodium starch glycolate. The loss of activity seemed to be related to the moisture uptake, free and bound water present in the bulk.

show abstract

Section: Aqueous Ph Stability Profile Of B Coagulansmentioning

confidence: 74%

Physicochemical Properties and Excipient Compatibility Studies of Probiotic Bacillus coagulans Spores

Bora¹,

Puri²,

Bansal³

2009

Sci. Pharm.

View full text Add to dashboard Cite

show abstract

“…The initial bacterial concentration was 10 7 CFU/ml and was checked by viable count determination on MRS broth as described below. Samples were incubated for 3 h at 37 C. Cells were serially diluted 10-fold in phosphate buffer (0.1 M, pH 7) in order to neutralize the medium acidity (Hyronimus et al, 2000). The residual viable count was determined by dilution and plate counting on MRS agar after 48 h of incubation.…”

Section: Methodsmentioning

confidence: 99%

“…Fermented milk products containing viable lactobacilli have been used by humans primarily as a prophylactic aid and their use has been extended for treatment of intestinal infections (Xanthopoulos et al, 2000). Thus, the basic conditions for lactic acid bacteria to be used as probiotics include the following: (i) they should survive and grow in the intestinal tract, (ii) be safe, (iii) maintain viability during processing and storage, (iv) be tolerant to acid and bile, and (v) produce organic acids (Hyronimus et al, 2000;Lin et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Conversion of squid pen by a novel strain Lactobacillus paracasei subsp. paracasei TKU010, and its application in antimicrobial and antioxidants activity

Liang

Huang

et al. 2010

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

TKU010 was isolated from infant vomited milk and identifi ed as Lactobacillus paracasei subsp. paracasei. TKU010 had desirable properties concerning its ability to withstand adverse conditions in the gastrointestinal tract. The hydrolysate of casein enhanced the growth of TKU010 most obviously (17.20 18.25 OD 660 ), followed by the hydrolysate of SPP (16.00 15.06 OD 660 ). Incubating with SPP, both the culture supernatant of TKU010 on the fi rst day and the fourth day showed inhibitory activities on E. coli BCRC13086, F. oxysporum BCRC32121 and A. fumigatus BCRC30099. TKU010 culture supernatant (1% SPP) incubated for 3 days has high antioxidant activity; the DPPH scavenging ability was 75% per ml. Thus, TKU010 could be preferably used as a starter to produce fermented milk with possibly interesting organoleptic properties. Besides, we have shown that squid pen wastes can be utilized to generate a high value-added product, and have revealed its hidden potential in the production of biocontrol agents and functional foods.

show abstract

“…It is a good candidate for probiotic use, produces organic acids and possesses the capacity to sporulate. It secretes a bacteriocin, coagulin, which has activity against a broad spectrum of enteric microbes [15][16][17]. Bacillus subtilis is also a Gram-positive, lactic acid-forming bacterial species.…”

Section: Introductionmentioning

confidence: 99%

Growth Promoting Potential and Colonization Ability of Probiotics (Bacillus coagulans and Bacillus subtilis) on the Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

Bhavan¹

2018

Insights Biol Med

View full text Add to dashboard Cite

The probiotic effects of Bacillus coagulans and Bacillus subtilis were studied on survival, growth, concentrations of basic biochemical constituents, activities of digestive enzymes, and their colony establishments in the gut of Macrobrachium rosenbergii post-larvae (PL). Eleven groups of PL (2.03±0.05 in length and 0.18±0.01g in weight), each consists of 35 individuals maintained in 25 L of ground water and fed ad libitum with fi ve serially diluted concentrations, 10 , 10 -7 and 10 -9 of B. coagulans, and B. subtilis incorporated diets containing 40% protein, for 45 days. Diet without incorporation of any of these probiotics was served as control. These probiotics were found to be alive in the respective feed even on day-15 after their formulations. Signifi cant improvement in survival, nutritional indices (weight gain, specifi c growth rate, food conversion ratio and protein effi ciency ratio), contents of basic biochemical constituents (total protein, amino acid, carbohydrate and lipid) and activities of digestive enzymes (protease, amylase and lipase) were observed (P<0.05), particularly in 10 -7 concentration of B. coagulans, and B. subtilis incorporated diets fed PL when compared with control. The biochemical confi rmation tests revealed that presence of Escherichia coli, Acetonobacter sp., Salmonella sp., and Pseudomonas sp., in the gut of control PL. In the gut of PL fed with B. coagulans incorporated diet, Acetonobacter sp., Salmonella sp., and Pseudomonas sp., were found to be competitively excluded, whereas, in the gut of PL fed with B. subtilis incorporated diet, Acetonobacter sp., and Salmonella sp., only were found to be excluded competitively. Actually, colonies of Bacillus sp., and Lactobacillus sp., were found to be establishment in the gut of PL fed with B. coagulans, and B. subtilis incorporated diets. Overall, these probiotics incorporated diets produced better growth and survival due to better FCR and activities of digestive enzymes, which in turn led to better nutritional profi le. Therefore they are recommended as feed additives for sustainable culture of M. rosenbergii.

show abstract

Acid and bile tolerance of spore-forming lactic acid bacteria

Cited by 259 publications

References 12 publications

Physicochemical Properties and Excipient Compatibility Studies of Probiotic Bacillus coagulans Spores

Physicochemical Properties and Excipient Compatibility Studies of Probiotic Bacillus coagulans Spores

Conversion of squid pen by a novel strain Lactobacillus paracasei subsp. paracasei TKU010, and its application in antimicrobial and antioxidants activity

Growth Promoting Potential and Colonization Ability of Probiotics (Bacillus coagulans and Bacillus subtilis) on the Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

Contact Info

Product

Resources

About