Acidic fibroblast growth factor is present in nonneural tissue: isolation and chemical characterization from bovine kidney

Gautschi-Sova, Peter; Jiang, Zhi Ping; Frater‐Schroeder, M.; Boehlen, Peter

doi:10.1021/bi00392a039

Cited by 69 publications

(18 citation statements)

References 22 publications

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“…On Western blot analysis of heparin-binding proteins extracted from extracellular matrices produced by myocyte cultures, a time-dependent accumulation of a 15-kDa protein immunologically identical to acidic FGF is observed. This protein is similar in size to acidic FGF-like proteins detected in brain (22), kidney (23), and aortic smooth muscle cells (24). No acidic FGF-like protein is detected in heparin-binding proteins extracted from extracellular matrices deposited by nonmyocyte cultures, further supporting the conclusion that acidic FGF is produced by the myocyte.…”

supporting

confidence: 61%

Acidic fibroblast growth factor mRNA is expressed by cardiac myocytes in culture and the protein is localized to the extracellular matrix.

Weiner

Swain

1989

Proc. Natl. Acad. Sci. U.S.A.

116

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Acidic fibroblast growth factor mRNA is expressed by cardiac myocytes in culture and the protein is localized to the extracellular matrix ( Communicated by James B. Wyngaarden, December 9, 1988 ABSTRACT Acidic and basic fibroblast growth factors are heparin-binding proteins that induce cellular proliferation, mesodermal development, and vascular growth. As such, they may be important in cardiac development and disease. To determine whether cardiac myocytes contain fibroblast growth factors, neonatal rat cardiac myocytes were studied in primary culture and compared to primary cultures of nonmyocyte cardiac cells. Northern blot analysis revealed a 4.0-kilobase mRNA in myocytes that hybridized to acidic fibroblast growth factor cDNA and was not detectable in nonmyocyte cultures. Western blot analysis demonstrated the accumulation of a 15-kDa peptide with immunological identity to acidic fibroblast growth factor in extracts of extracellular matrix from myocyte cultures that was not detectable in similar extracts of nonmyocyte extracellular matrix. No acidic fibroblast growth factorlike protein was detectable in cellular lysates from either myocyte or nonmyocyte cultures. These results demonstrate that neonatal cardiac myocytes express acidic fibroblast growth factor mRNA and deposit a protein with immunological identity to acidic fibroblast growth factor into the extracellular matrix. The results suggest that acidic fibroblast growth factor produced by cardiac myocytes may mediate, through both paracrine and autocrine mechanisms, such diverse processes as myocyte differentiation, cellular proliferation, and vascular growth in the heart.

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supporting

confidence: 61%

Acidic fibroblast growth factor mRNA is expressed by cardiac myocytes in culture and the protein is localized to the extracellular matrix.

Weiner

Swain

1989

Proc. Natl. Acad. Sci. U.S.A.

116

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“…HBGF-1 protein was isolated from bovine kidney (20), human brain (21,23,27), smooth muscle cells (55), and various glioma cell lines (32). To see whether mRNA was present in these tissues and cell lines, we used the 2.6-kbp BglII-BglIll fragment to detect the HBGF-1 mRNA by Northern blotting and hybridization.…”

Section: Resultsmentioning

confidence: 99%

“…HBGF-2 has been isolated from a variety of tissues and tumor cell lines (25,35), whereas the sources of HBGF-1 have included neural tissues such as brain, hypothalamus, and retina (34,35). Recently, HBGF-1 was isolated from bovine kidney (20), human smooth muscle cells (55), and glioma cells (32). It has been postulated that HBGF-1 may stimulate cells to grow through an autocrine pathway (32,55).…”

mentioning

confidence: 99%

Cloning of the gene coding for human class 1 heparin-binding growth factor and its expression in fetal tissues.

et al. 1989

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We have identified four overlapping genomic DNA clones coding for human class 1 heparin-binding growth factor (HBGF-1), also known as acidic fibroblast growth factor, by screening genomic DNA libraries with an HBGF-1 cDNA probe. The exon-intron structure of the HBGF-1 gene was determined by Southern hybridization and nucleotide sequence analysis. The complete amino acid sequence of human HBGF-1 was deduced from the nucleotide sequence of these genomic DNA clones. The predicted amino acid sequence is identical to the published amino acid sequence determined by protein sequencing. Southern blot analysis of human DNA suggested that there is a single-copy gene coding for HBGF-1. A 4.5-kilobase mRNA and two minor species (3.4 and 2.0 kilobases) homologous to the HBGF-1 gene were detected in cellular RNA isolated from human adult brain and kidney. The HBGF-1 mRNAs from brain and kidney had slightly different sizes. The mechanism for the synthesis of different sizes of mRNA was not determined. We also detected HBGF-1 transcript from glioblastoma cells, fetal brain, and kidney but not from placenta or fetal liver. Since HBGF-1 is an angiogenic factor, these data suggest that it may play a role in embryonic angiogenesis during fetal development.

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“…(X65.) bovine kidney has been used in the past for purification purposes as an enriched source of both FGF2 (25) and FGF1 (29). In the developing metanephros, an undefined heparinbinding protein of 16-20 kDa with angiogenic activity has been identified in mouse renal rudiments at gdll, which is the developmental equivalent of gdl3 in rat, and represents a preinduction state in renal organogenesis (30 (38), it has also been shown to regulate cell adhesion (39) (43), and (25,43) adult tissues.…”

Section: Methodsmentioning

confidence: 99%

Basic fibroblast growth factor can mediate the early inductive events in renal development.

Perantoni

Dove

Karavanova

1995

Proc. Natl. Acad. Sci. U.S.A.

152

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METHODSTissues. Timed pregnant F344 rats were euthanized at gestational day 13 (gdl3; the day a spermatic plug was observed was designated as gdO), and embryonic kidneys were surgically excised in phosphate-buffered saline. Metanephrogenic mesenchymes were then isolated from the buds and cultured as described (8). Only mesenchymes from rudiments in which the bud had just begun its primary branching were used, since at this stage it has not been induced to form tubules.Purification of Condensing Activity. Bovine pituitary extract was prepared for induction studies as described (15)

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Acidic fibroblast growth factor is present in nonneural tissue: isolation and chemical characterization from bovine kidney

Cited by 69 publications

References 22 publications

Acidic fibroblast growth factor mRNA is expressed by cardiac myocytes in culture and the protein is localized to the extracellular matrix.

Acidic fibroblast growth factor mRNA is expressed by cardiac myocytes in culture and the protein is localized to the extracellular matrix.

Cloning of the gene coding for human class 1 heparin-binding growth factor and its expression in fetal tissues.

Basic fibroblast growth factor can mediate the early inductive events in renal development.

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