Acne is Not Associated with Yet-Uncultured Bacteria

Bek-Thomsen, Malene; Lomholt, Hans; Kilian, Mogens

doi:10.1128/jcm.00799-08

Cited by 131 publications

(109 citation statements)

References 38 publications

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“…In general, all case–control studies demonstrated similarly an increased microbial abundance of Propionibacterium acnes in the skin microbiome of patients with AV, compared to healthy 51, 52, 53. In addition, an association between a specific P. acnes strains and acne affected skin, and healthy skin respectively was demonstrated 51, 52. Acne improved and Propionibacterium abundance decreased after various treatments, together with an increase of microbial diversity in the two controlled studies.…”

Section: Resultsmentioning

confidence: 56%

“…Three (3) were case–control studies and two (2) were small single‐centre, controlled studies, of whom one was a double‐blind, randomized‐controlled trial. In general, all case–control studies demonstrated similarly an increased microbial abundance of Propionibacterium acnes in the skin microbiome of patients with AV, compared to healthy 51, 52, 53. In addition, an association between a specific P. acnes strains and acne affected skin, and healthy skin respectively was demonstrated 51, 52.…”

Section: Resultsmentioning

confidence: 59%

“…Five studies investigated the skin microbiome in patients with AV, Table 1 51, 52, 53, 54, 55. Three (3) were case–control studies and two (2) were small single‐centre, controlled studies, of whom one was a double‐blind, randomized‐controlled trial.…”

Section: Resultsmentioning

confidence: 99%

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A systematic literature review of the human skin microbiome as biomarker for dermatological drug development

Kolk

Wall

Balmforth

et al. 2018

Brit J Clinical Pharma

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AimsTo explore the potential of the skin microbiome as biomarker in six dermatological conditions: atopic dermatitis (AD), acne vulgaris (AV), psoriasis vulgaris (PV), hidradenitis suppurativa (HS), seborrhoeic dermatitis/pityriasis capitis (SD/PC) and ulcus cruris (UC).MethodsA systematic literature review was conducted according to the PRISMA guidelines. Two investigators independently reviewed the included studies and ranked the suitability microbiome implementation for early phase clinical studies in an adapted GRADE method.ResultsIn total, 841 papers were identified and after screening of titles and abstracts for eligibility we identified 42 manuscripts that could be included in the review. Eleven studies were included for AD, five for AV, 10 for PV, two for HS, four for SD and 10 for UC. For AD and AV, multiple studies report the relationship between the skin microbiome, disease severity and clinical response to treatment. This is currently lacking for the remaining conditions.ConclusionFor two indications – AD and AV – there is preliminary evidence to support implementation of the skin microbiome as biomarkers in early phase clinical trials. For PV, UC, SD and HS there is insufficient evidence from the literature. More microbiome‐directed prospective studies studying the effect of current treatments on the microbiome with special attention for patient meta‐data, sampling methods and analysis methods are needed to draw more substantial conclusions.

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Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 59%

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A systematic literature review of the human skin microbiome as biomarker for dermatological drug development

Kolk

Wall

Balmforth

et al. 2018

Brit J Clinical Pharma

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“…Antibacterial activity test from ethyl acetate fraction of green tea leaves extract with buffer solution pH 4 methods in Propionibacterium acnes ATCC 6919: Propionibacterium acnes ATCC 6919 is a Gram-positive bacterium which is a facultative anaerobic bacteria that cause acne specific (Bek-Thomsen et al, 2008).…”

Section: Phosphate Acid (%) -----------------------------------------mentioning

confidence: 99%

Stability of Epigallocatechin Gallate (EGCG) from Green Tea (Camellia sinensis) and its Antibacterial Activity against Staphylococcus epidermidis ATCC 35984 and Propionibacterium acnes ATCC 6919

Widyaningr¹,

Fudholi²,

Sudarsono³

et al. 2015

Asian J. of Biological Sciences

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One of problem from anti acne cream formula containing Epigallocatechin gallate (EGCG) from green tea leaves is EGCG stability. The EGCG is unstable to heat and easily oxidated. However, EGCG is the main marker substance related to antibacterial activity. This study aimed to determine the EGCG stability and its antibacterial activity. The EGCG stability testing was obtained during extraction process by adding phosphoric acid buffer, pH 4 buffer solution and control. The stability of these groups then tested at 2, 25 and 40°C temperature. Evaluation for absorbance changing by UV-VIS spectrophotometry was obtained during 15 days. After that, EGCG concentration from the three groups were compared using HPLC. Antibacterial activity testing for acne causing bacteria Staphylococcus epidermidis ATCC 35984 was conducted from phosphoric acid and pH 4 buffer solution groups. The result showed that extreme cold temperature decreasing treatment against green tea leaves extract after infundation, pH 4 buffer solution adding and storage at temperature of 2°C produced the highest EGCG concentration namely 60.98% w/w. Besides that, antibacterial activity from the ethyl acetate fraction of green tea leaves extract by using this method produced a good inhibition against P. acnes and S. epidermidis. Moreover, the antibacterial activity against P. acnes was higher than S. epidermis. The adding of pH 4 buffer solution in extraction process produced the highest EGCG concentration than phosphoric acid adding and no buffer adding. The EGCG was stable at the storage temperature of 2°C and 6% ethyl acetate fraction of green tea leaves extract can be used as an anti acne dosage.

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“…These results clearly exclude the possibility that yet-uncultured bacteria are associated with acne-affected skin follicles. [2] Propionibacterium acnes play a central role in acne pathogenesis. Not only does this anaerobic bacterium produce lipases, proteases, and other extracellular enzymes, it also secretes chemotactic factors attracting polymorphonuclear leukocytes, lymphocytes, and macrophages.…”

Section: Discussionmentioning

confidence: 99%

Bacteriology of Acne

Srikanth¹,

Kalyani²,

Mohan³

et al. 2015

jemds

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CONTEXT: Acne vulgaris is the most common skin disorder in youth and is of multifactorial etiology. Severe forms of acne are often therapy resistant. AIMS: To determine bacteria involved in acne vulgaris and to determine the in vitro antibiotic sensitivity of aerobic isolates. SETTINGS AND DESIGN: Cross sectional study. METHODS AND MATERIALS: Samples from skin lesions of study group (80 patients) and normal skin of control group (20 patients) were collected by sterile swabs. The specimens were used for aerobic and anaerobic culture at 37°C. The isolates were subjected to biochemical tests for identification. All the aerobic isolates were subjected to antimicrobial sensitivity testing by disc diffusion method. RESULTS: High incidence of acne was found in the age group of 13-20 years (76.25%). Males were more affected than females. Pustules (76.25%) were the commonest presentation. Students (71.25%) were more commonly affected. Among aerobic isolates (71.24%), Staphylococcus epidermidis (54.38%) was the most common organism. Among the anaerobes, Propionibacterium acnes (55.17) were the most common. Among the aerobic isolates, most were sensitive to Minocycline followed by Ofloxacin, Azithromycin, Cephalexin, Tetracycline, Cotrimoxazole and Ampicillin. CONCLUSIONS: In the present study, acne affected individuals were young males, especially students, with pustules being the commonest presentation. Staphylococcus epidermidis, the dominant organism isolated was susceptible to Ofloxacin, Minocycline and Azithromycin.

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Acne is Not Associated with Yet-Uncultured Bacteria

Cited by 131 publications

References 38 publications

A systematic literature review of the human skin microbiome as biomarker for dermatological drug development

A systematic literature review of the human skin microbiome as biomarker for dermatological drug development

Stability of Epigallocatechin Gallate (EGCG) from Green Tea (Camellia sinensis) and its Antibacterial Activity against Staphylococcus epidermidis ATCC 35984 and Propionibacterium acnes ATCC 6919

Bacteriology of Acne

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