Stability of Epigallocatechin Gallate (EGCG) from Green Tea (Camellia sinensis) and its Antibacterial Activity against Staphylococcus epidermidis ATCC 35984 and Propionibacterium acnes ATCC 6919

Abstract

One of problem from anti acne cream formula containing Epigallocatechin gallate (EGCG) from green tea leaves is EGCG stability. The EGCG is unstable to heat and easily oxidated. However, EGCG is the main marker substance related to antibacterial activity. This study aimed to determine the EGCG stability and its antibacterial activity. The EGCG stability testing was obtained during extraction process by adding phosphoric acid buffer, pH 4 buffer solution and control. The stability of these groups then tested at 2, 25 and 40°C temperature. Evaluation for absorbance changing by UV-VIS spectrophotometry was obtained during 15 days. After that, EGCG concentration from the three groups were compared using HPLC. Antibacterial activity testing for acne causing bacteria Staphylococcus epidermidis ATCC 35984 was conducted from phosphoric acid and pH 4 buffer solution groups. The result showed that extreme cold temperature decreasing treatment against green tea leaves extract after infundation, pH 4 buffer solution adding and storage at temperature of 2°C produced the highest EGCG concentration namely 60.98% w/w. Besides that, antibacterial activity from the ethyl acetate fraction of green tea leaves extract by using this method produced a good inhibition against P. acnes and S. epidermidis. Moreover, the antibacterial activity against P. acnes was higher than S. epidermis. The adding of pH 4 buffer solution in extraction process produced the highest EGCG concentration than phosphoric acid adding and no buffer adding. The EGCG was stable at the storage temperature of 2°C and 6% ethyl acetate fraction of green tea leaves extract can be used as an anti acne dosage.

Antibacterial activity of the dregs of green tea leaves (Camellia sinensis L.) on Staphylococcus epidermidis as causes of acne

“…Based on the antibacterial activity of S. epidermidis test of the dregs of green tea leaves in our study, it proved that the dregs leaf green tea has anti-acne activity at a concentration of 1% in inhibiting the survival of S. epidermidis. These results are consistent with research by Widyaningrum et al (2015a) which stated ethyl acetate fraction of green tea leaf extract concentration of 1% to 6% have antibacterial activity on P. acnes and S. epidermidis and concentration of 1 to 9 % have antibacterial activity on Staphylococcus aureus (Widyaningrum, 2013), so the concentration of 1% is recommended as a dose concentration of anti acne. This shows that the leaves of green tea leaves can be used as an alternative in overcoming acne.…”

“…It was done with infundation method by using distilled water with a temperature of 90°C for 30 minutes, then an extreme cold and the addition of Dapar solution to pH 4 were performed (Widyaningrum et al, 2015a).…”

“…The research of Widyaningrum et al (2015a) also stated that ethyl acetate fraction of green tea leaf extract concentration of 1% to 6% has antibacterial activity on P. acnes and Staphylococcus epidermidis (S. epidermidis). The concentration of 6% is recommended as a dose concentration of anti-acne.…”

Optimization of a Novel Kinetic-Assisted Infundation for Rich-Egcg and Polyphenols of White Tea (Camellia Sinensis) Using Central Composite Design

“…In order to obtain high levels of EGCG and polyphenols, a strategy is needed in regulating the material ratio with solvent, temperature, stirring rate, and extraction time. In various literature, EGCG and catechin derivatives will be easily damaged at temperatures above 100 °C [10,12,13]. However, at temperatures lower than 100 °C for a long time, the EGCG obtained decreases [11,21].…”

“…Kinetic-assisted infundation (KAI) is an extraction technique developed from conventional infundation (CIn) methods. Conventional methods have disadvantages such as slow-moving compound transfer processes caused by low solvent penetration [10,11]. In other conditions, advanced methods such as ultrasoundassisted extraction (UAE) and microwave-assisted extraction (MAE) are not necessarily suitable for the extraction of active compounds from white tea.…”