Acoustically evoked potentials in two cephalopods inferred using the auditory brainstem response (ABR) approach

Hu, Marian Yong-An; Yan, Hong; Chung, Wen-Sung; Shiao, Jen‐Chieh; Hwang, Pung‐Pung

doi:10.1016/j.cbpa.2009.02.040

Cited by 55 publications

(52 citation statements)

References 24 publications

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“…Recent laboratory experiments have demonstrated that squid do not exhibit anti-predator responses in the presence of odontocete echolocation clicks (Wilson et al 2007) indicating that they cannot detect the ultrasonic pressure component of a sound field. However, recently, Hu et al (2009) suggested that squid (Sepiotheutis lessoniana) can detect sound pressure stimuli using their statocyst organ. Unfortunately, these data had several methodological issues including no calibrations of particle motion and placing squid at the water's surface where discrepancies between sound pressure and particle motion are greatest.…”

Section: The Sensitivity Of Squid To Acoustic Stimulimentioning

confidence: 99%

Potential for Sound Sensitivity in Cephalopods

Mooney

Hanlon

Madsen

et al. 2012

Advances in Experimental Medicine and Biology

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Section: The Sensitivity Of Squid To Acoustic Stimulimentioning

confidence: 99%

Potential for Sound Sensitivity in Cephalopods

Mooney

Hanlon

Madsen

et al. 2012

Advances in Experimental Medicine and Biology

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“…Prior studies have shown the squid statocysts to be key sensory organs that may detect acceleration and can transduce detection of sound stimuli (Budelmann, 1990;Packard et al, 1990;Hu et al, 2009;Mooney et al, 2010). These studies combined with the findings presented here make it attractive to suggest squid hair cells as an invertebrate model system to study hair cell physiology and pharmacology.…”

Section: Discussionmentioning

confidence: 55%

“…However, those studies examined lateral line hair cells, which were bath-exposed to neomycin, as opposed to the encapsulated vestibular system hair cells analyzed in our study, making comparisons with our treatment protocol difficult. Lower concentrations of neomycin treatment (0.8 mmol l Ϫ1 ) do appear to impact statocyst physiological responses in the oval squid, Sepioteuthis lessoniana, extinguishing acoustically evoked potentials between 20 and 30 min after methodologically similar injections into the statocyst (Hu et al, 2009). However, Hu et al did not examine morphological changes, and they suggest that the extinction of response may have been due to brain death as opposed to hair cell damage.…”

Section: Discussionmentioning

confidence: 99%

“…The statocyst is analogous to the fish otolith system (Fay, 1974) and, to some extent, may share functions of the inner ear hair cells of higher vertebrates (Yost, 1994). Because squid are now considered to respond to some acoustic components, there is concern that increases in ocean noise may damage statocyst hair cells and impact their hearing (Packard et al, 1990;Budelmann et al, 1992;Kaifu et al, 2008;Hu et al, 2009;André et al, 2011;Mooney et al, 2012;Solé et al, 2013). Additionally, squid have been a model species in physiology and neuroscience since the classic experiments by Hodgkin and Huxley on the nature of the action potential Huxley, 1945, 1952), and their use is growing in other areas such as the study of camouflage and animal-bacterial symbioses (Hanlon, 2007;Lee et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Aminoglycoside-Induced Damage in the Statocyst of the Longfin Inshore Squid, Doryteuthis pealeii

Scharr

Mooney

Schweizer

et al. 2014

The Biological Bulletin

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Abstract. Squid are a significant component of the marine biomass and are a long-established model organism in experimental neurophysiology. The squid statocyst senses linear and angular acceleration and is the best candidate for mediating squid auditory responses, but its physiology and morphology are rarely studied. The statocyst contains mechano-sensitive hair cells that resemble hair cells in the vestibular and auditory systems of other animals. We examined whether squid statocyst hair cells are sensitive to aminoglycosides, a group of antibiotics that are ototoxic in fish, birds, and mammals. To assess aminoglycoside-induced damage, we used immunofluorescent methods to image the major cell types in the statocyst of longfin squid (Doryteuthis pealeii). Statocysts of live, anesthetized squid were injected with either a buffered saline solution or neomycin at concentrations ranging from 0.05 to 3.0 mmol l Ϫ1 . The statocyst hair cells of the macula statica princeps were examined 5 h post-treatment. Anti-acetylated tubulin staining showed no morphological differences between the hair cells of saline-injected and non-injected statocysts. The hair cell bundles of the macula statica princeps in aminoglycoside-injected statocysts were either missing or damaged, with the amount of damage being dose-dependent. The proportion of missing hair cells did not increase at the same rate as damaged cells, suggesting that neomycin treatment affects hair cells in a nonlethal manner. These experiments provide a reliable method for imaging squid hair cells. Further, aminoglycosides can be used to induce hair cell damage in a primary sensory area of the statocyst of squid. Such results support further studies on loss of hearing and balance in squid.

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“…Cephalopods exhibit a high degree of cephalization and a complex neural system with very efficient sensory organs such as lens eyes, chemo-receptors and balance receptors. 1,2 It is believed that these vertebrate-like features arose from the competition with fish since their first occurrence during the so called "Cambrian explosion" around 500 million years ago. 3,4 Although many features of fish and cephalopods have been described as convergent, fundamental anatomical and physiological differences of cephalopods constrain their evolutionary competition with fish.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in understanding trans-epithelial acid-base regulation and excretion mechanisms in cephalopods

Hwang

Tseng

2015

Tissue Barriers

Self Cite

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Cephalopods have evolved complex sensory systems and an active lifestyle to compete with fish for similar resources in the marine environment. Their highly active lifestyle and their extensive protein metabolism has led to substantial acid-base regulatory abilities enabling these organisms to cope with CO 2 induced acid-base disturbances. In convergence to teleost, cephalopods possess an ontogeny-dependent shift in ion-regulatory epithelia with epidermal ionocytes being the major site of embryonic acid-base regulation and ammonia excretion, while gill epithelia take these functions in adults. Although the basic morphology and excretory function of gill epithelia in cephalopods were outlined almost half a century ago, modern immunohistological and molecular techniques are bringing new insights to the mechanistic basis of acidbase regulation and excretion of nitrogenous waste products (e.g. NH 3 /NH 4 C ) across ion regulatory epithelia of cephalopods. Using cephalopods as an invertebrate model, recent findings reveal partly conserved mechanisms but also novel aspects of acid-base regulation and nitrogen excretion in these exclusively marine animals. Comparative studies using a range of marine invertebrates will create a novel and exciting research direction addressing the evolution of pH regulatory and excretory systems.

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Acoustically evoked potentials in two cephalopods inferred using the auditory brainstem response (ABR) approach

Cited by 55 publications

References 24 publications

Potential for Sound Sensitivity in Cephalopods

Potential for Sound Sensitivity in Cephalopods

Aminoglycoside-Induced Damage in the Statocyst of the Longfin Inshore Squid, Doryteuthis pealeii

Recent advances in understanding trans-epithelial acid-base regulation and excretion mechanisms in cephalopods

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