Acridine orange coated magnetic nanoparticles for nucleus labeling and DNA adsorption

Liu, Chi-Hsien; Sahoo, Soubhagya Laxmi; Tsao, Min-Han

doi:10.1016/j.colsurfb.2013.11.003

Cited by 25 publications

(16 citation statements)

References 39 publications

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“…Functionalized MNPs can label cell nucleus through the specic absorption between DNA and acridine on MNPs and can emit uorescence aer DNA binding. 18 These studies support the nding that uorescent nanoparticles can facilitate various purposes, such as tracking, sensing, imaging, and labeling. Despite the extensive applications of Hoechst dye for DNA staining, uncertainty still remains concerning the dye's photo-stability and intense washes aer staining.…”

Section: Introductionmentioning

confidence: 57%

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

et al. 2017

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Section: Introductionmentioning

confidence: 57%

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

et al. 2017

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“…Tanaka et al (34) analyzed 94 primary lung cancer patients without distant metastasis and 31 primary lung cancer patients with distant metastasis using the CELLSEARCH ® System, and found that there was a significant difference in CTC counts between them and demonstrated that CTCs were the main indicator of distant metastasis. However, the diagnostic significance of CTCs was controversial in various previous studies (13,32,33), and its value requires further investigation.…”

Section: Discussionmentioning

confidence: 96%

“…The sensitivity of this method was inferior to that of the method used in the present study, as reported previously (27,28). Various steps, including centrifugation, counting, and rinsing, as well as the inherent characteristics of AO-F, such as the weak photostability of free AO-F, may cause cell loss to varying degrees (32). Furthermore, the present study found a positive correlation between the number of AO-F-positive cells and the total number of cells in the system.…”

Section: Discussionmentioning

confidence: 99%

New applications of the acridine orange fluorescence staining method: Screening for circulating tumor cells

et al. 2017

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The aim of the present study was to explore use of the acridine orange fluorescence (AO-F) staining method for screening of circulating tumor cells (CTCs) in renal cell carcinoma (RCC) patients. The AO-F positive staining rate of live and dead tumor cells was calculated. The positive staining rate in the live group was 93.4±3.0%, while the dead group failed to emit specific fluorescence. A known number of tumor cells were added to peripheral blood, and the detection sensitivity of the four groups (50, 100, 200 and 500 cells/tube) was 10.2±3.8, 9.2±2.3, 10.8±2.6 and 10.5±1.9%, respectively. The average detection sensitivity of the four groups was 10.16±2.73%. There was a positive correlation between the number of cells that was positively stained with AO-F and the total number cells in the system (χ2=0.959; P<0.001). Subsequently, the AO-F staining method was used to detect positive staining cells in 8 healthy volunteers (control group), and 112 non-metastatic and 27 metastatic RCC patients. The positive staining rate was 13.67% (19/139) in RCC patients, while none of the control group was positive. The AO-F positive staining rate was not significantly different between the metastatic and non-metastatic patients according to age, gender, the pathological pattern, T2/3 (according to the Tumor-Node-Metastasis classification) or Fuhrman grade, while there was a significant difference according to T1. The positive staining rate was 8.93% (10/112) for non-metastatic patients and 33.33% (9/27) for metastatic patients, which showed a significant difference (P<0.05). In 112 non-metastatic and 27 metastatic patients, the positive staining rate was not significantly associated with gender, age, tumor size, the pathological pattern, T classification, Fuhrman grade, the presence of a lesion or metastasis to the lungs. The present study demonstrated that the method of CTC staining with AO-F, which has high reproducibility and specificity, was feasible for identifying CTCs and warrants further study.

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“…It has excellent potential for physiological applications such as targeted drug and gene delivery [6,25,26]，cell and protein separation [8,27], magnetic resonance (MR) imaging [5,28] and cancer therapy (hyperthermia) [7,29]. The MNPs could be flexibly designed and synthesized as multifunctional nanoparticles via modifying ligands on the surface according to different application purposes [30,31].…”

Section: Introductionmentioning

confidence: 99%

Multifunctional fluorescent magnetic nanoparticles for lung cancer stem cells research

Zhou

Chen

Wang

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Acridine orange coated magnetic nanoparticles for nucleus labeling and DNA adsorption

Cited by 25 publications

References 39 publications

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

Magnetic nanoparticles with fluorescence and affinity for DNA sensing and nucleus staining

New applications of the acridine orange fluorescence staining method: Screening for circulating tumor cells

Multifunctional fluorescent magnetic nanoparticles for lung cancer stem cells research

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