Acroptilon repens induces apoptosis in human breast adenocarcinoma

Abstract

Evaluating the effect of herbal extracts has been always interesting for cancer researchers considering that these natural materials could be suitable sources for finding new anti-cancer agents. In the present study, Acroptilon repens methanol extract had been evaluated for its cytotoxic effects in two human breast cancer cell lines, MCF-7 and MDA-MB-468, using MTT assay. The apoptosis potential had also been evaluated using annexin-V/propidium iodide assay, Hoechst 33258 staining and evaluating the cell cycle with flow cytometery. The MTT results showed cytotoxicity with IC50 values of 69.2 and 32.6 μg/mL for MCF-7 and MDA-MB-468 cells, respectively. The results of the apoptosis assays confirmed the apoptosis potential of the plant extract in the breast cancer cell lines suggesting A. repens for further cancer studies.

Phytochemical identification, acute and subchronic oral toxicity assessments of hydroalcoholic extract of Acroptilon repens in BALB/c mice: A toxicological and mechanistic study

“…These proteins may cause loss of the mitochondrial membrane potential (ΔΨ) and the swelling of the matrix of mitochondria through the formation of membrane pores or an increase in the permeability of the mitochondrial membrane, finally causing apoptotic effectors to leak out [36,37]. As shown in the result (Figures 8, 9, and 10 a and b), most doses of 500 and 1000 mg/kg of hydroalcoholic extract in the subchronic study cause mitochondrial damages in mice livers and brains, suggesting that hydroalcoholic extract induces apoptosis [38]. Several studies confirm that mitochondrial dysfunction conveys the apoptosis process [39,40].…”

“…As shown in the result (Figures 8 , 9 , and 10 a and b), most doses of 500 and 1000 mg/kg of hydroalcoholic extract in the subchronic study cause mitochondrial damages in mice livers and brains, suggesting that hydroalcoholic extract induces apoptosis [ 38 ]. Several studies confirm that mitochondrial dysfunction conveys the apoptosis process [ 39 , 40 ].…”

Effect of Afghan Talkhak (Acroptilon Repens) Aqueous Extract on Blood Glucose Level in Diabetic Rats

“…However, there is not much more evidence of the biological activities of A. Repens (22,23). Thus, this study initiates to evaluate the effect of its root extract on the blood glucose level of diabetic rats, using a streptozotocin-induced model of experimental diabetes.…”