Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection

Bossé, Janine T.; Janson, Håkan; Sheehan, Brian J.; Beddek, Amanda J.; Rycroft, Andrew N.; Kroll, J. Simon; Langford, Paul R.

doi:10.1016/s1286-4579(01)01534-9

Cited by 321 publications

(308 citation statements)

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“…Symptoms of the disease range broadly from pre-acute to chronic, and clinical signs include fever, dyspnea, anorexia, vomiting, coughing, frothy hemorrhage, diarrhea, and cyanosis (Boss e et al 2002). In the acute stage, the disease is characterized by hemorrhagic necrotizing pneumonia and fibrinous pleuritis, while the chronic stage involves localized lung lesions and adhesive pleuritis (Haesebrouck et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In the acute stage, the disease is characterized by hemorrhagic necrotizing pneumonia and fibrinous pleuritis, while the chronic stage involves localized lung lesions and adhesive pleuritis (Haesebrouck et al 2004). This disease affects pigs of all ages and has a major impact on the economics, ecology, and animal welfare in the pigrearing industry (Boss e et al 2002). It is essential that an appropriate therapy be applied as accurately and quickly as possible.…”

Section: Introductionmentioning

confidence: 99%

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Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Kim

Hur

Lee

et al. 2016

Veterinary Quarterly

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Background: Actinobacillus pleuropneumoniae (APP) causes porcine pleuropneumonia (PP). Objective: Serotypes and antimicrobial resistance patterns in APP isolates from pigs in Korea were examined. Methods: Sixty-five APP isolates were genetically serotyped using standard and multiplex PCR (polymerase chain reaction). Antimicrobial susceptibilities were tested using the standardized disk-agar method. PCR was used to detect b-lactam, gentamicin and tetracycline-resistance genes. The random amplified polymorphic DNA (RAPD) patterns were determined by PCR. Results: Korean pigs predominantly carried APP serotypes 1 and 5. Among 65 isolates, one isolate was sensitive to all 12 antimicrobials tested in this study. Sixty-two isolates was resistant to tetracycline and 53 isolates carried one or five genes including tet(B), tet(A), tet(H), tet(M)/tet (O), tet(C), tet(G) and/or tet(L)-1 markers. Among 64 strains, 9% and 26.6% were resistance to 10 and three or more antimicrobials, respectively. Thirteen different antimicrobial resistance patterns were observed and RAPD analysis revealed a separation of the isolates into two clusters: cluster II (6 strains resistant to 10 antimicrobials) and cluster I (the other 59 strains). Conclusion: Results show that APP serotypes 1 and 5 are the most common in Korea, and multidrug resistant strains are prevalent. RAPD analysis demonstrated that six isolates resistant to 10 antimicrobials belonged to the same cluster.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Kim

Hur

Lee

et al. 2016

Veterinary Quarterly

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“…On the basis of the β-nicotinamide adenine dinucleotide (β-NAD) requirement for growth A. pleuropneumoniae has been classified into biovar 1 (β-NAD dependent) and biovar 2 (β-NAD independent). Biovar 2 can synthesize NAD in the presence of specific pyridine nucleotides or their precursors (6,21). Each biovar is further divided into serovars.…”

Section: Praca Oryginalnamentioning

confidence: 99%

Antimicrobial susceptibility of Actinobacillus pleuropneumoniae strains isolated from pig's clinical samples in Serbia

Žutić¹,

Pavlovic²,

Radanović³

et al. 2016

Medycyna Weterynaryjna

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SummaryPorcine pleuropneumonia, caused by Actinobacillus pleuropneumoniae is one of the most important bacterial diseases of the pig respiratory tract. One hundred and forty eight Actinobacillus pleuropneumoniae strains isolated from pig's clinical samples were tested for their susceptibility to 11 antimicrobials by disk diffusion method. Isolates were obtained from the pathoanatomically altered pig's lung that died with signs of respiratory disease. Antimicrobial discs with following concentration were used: ampicillin 10 µg, amoxicillin 10 µg, amoxicillin/clavulanic acid 20/10 µg, cefotaxime 30 µg, gentamicin 10 µg, penicillin 10 IU, streptomycin 10 µg, tetracycline 30 µg, enrofloxacin 5 µg, lincospectin 15/200 µg, florfenicol 30 µg and trimethoprim/sulfamethoxazole 1.25/23.75 µg. Isolated strains showed various susceptibility to tested antimicrobials. Seventy-five percent of isolates were resistant to one or more drugs frequently used in the treatment of pig's diseases at farms in Serbia. All 148 isolates were susceptible to cefotaxime, enrofloxacin and florfenicol, while 96% of them were susceptible to amoxicillin/clavulanic acid and lincospectin. Susceptibility of isolates to amoxicillin, gentamicin and trimethoprim/sulfamethoxazole were 91%, 77% and 64%, respectively. The highest resistance rates were determined for tetracycline and streptomycin, 34% and 31%, respectively. When compared to our previous results, we noticed a decrease in resistance to tetracyclines and trimethoprim/sulfamethoxazole. Our results indicate that A. pleuropneumoniae strains isolated from pig's clinical samples in Serbia show high susceptibility to antimicrobial agents which are most often used in the pig industry.

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“…A pleuropneumonia suína (PPS) causada pela bactéria Actinobacillus pleuropneumoniae (App), provoca doença clí-nica caracterizada por pneumonia com pleurisia fibrinosa, lesões pulmonares necrohemorrágicas, adesões pleurais fibrinóticas e, em casos severos, à morte (Bossé et al 2002). O contágio geralmente ocorre após inalação de aerossóis ou contato direto, o microrganismo inalado coloniza o tecido pulmonar aderindo-se ao muco, proteínas e a células hospedeiras com posterior multiplicação no local (Chiers et al 2010).…”

Section: Introductionunclassified

“…São consideradas doenças multifatoriais, provocando significativas perdas econômicas, geralmente associadas à produção intensiva, a fatores ambientais e de manejo (Hansen et al 2010, Opriessnig, Gimenez-Lirola & Halbur 2011. O diagnóstico baseia-se no isolamento do agente a partir de lesões suspeitas e, principalmente, em testes sorológicos para estabelecimento de medidas de controle (Coelho et al 2004, Xie et al 2013.A pleuropneumonia suína (PPS) causada pela bactéria Actinobacillus pleuropneumoniae (App), provoca doença clí-nica caracterizada por pneumonia com pleurisia fibrinosa, lesões pulmonares necrohemorrágicas, adesões pleurais fibrinóticas e, em casos severos, à morte (Bossé et al 2002). O contágio geralmente ocorre após inalação de aerossóis ou contato direto, o microrganismo inalado coloniza o tecido pulmonar aderindo-se ao muco, proteínas e a células hospedeiras com posterior multiplicação no local (Chiers et al 2010).…”

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Padronização da técnica de nanopartícula de ouro não modificada (AuNPs) para detecção de Actinobacillus pleuropneumoniae em pulmões de suínos

et al. 2014

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Standardization of unmodified gold nanoparticle (AuNPs) for detection of Actinobacillus pleuropneumoniae in swine lungs.] Padronização da técnica de nanopartícula de ouro não modificada (AuNPs) para detecção de Actinobacillus pleuropneumoniae em pulmões de suínos. Pesquisa Veterinária Brasileira 34(7):621-625. Laboratório de Microbiologia e Biologia Molecular, Universidade Federal do Mato Grosso, Av. Fernando Corrêa da Costa2367, Bairro Boa Esperança, Cuiabá, MT 78060-900, Brazil. E-mail: valdutra@ufmt.br Based on diagnostic tests for the detection of nucleic acids without amplification through the use of gold nanoparticles (AuNPs) have been described for various diseases. This study aimed to develop a technique of unmodified AuNPs to detect Actinobacillus pleuropneumoniae (App). We used 70 lung samples from pigs, 17 with and 53 without characteristic lesions of pneumonia, to detect App. The primer used was based on ApxIV gene. The AuNPs test had a sensitivity of 93.8% and specificity of 84.6% when compared with PCR detection. The results showed good agreement between AuNPs and PCR testing, and the technique can be used as an alternative to conventional tests, since it is quick and easy, and does not require implementation infrastructure and skilled labor.INDEX TERMS: Swine pneumonia, gold nanoparticle, AuNP, Actinobacillus pleuropneumoniae. (Rossi et al. 2013). São consideradas doenças multifatoriais, provocando significativas perdas econômicas, geralmente associadas à produção intensiva, a fatores ambientais e de manejo (Hansen et al. 2010, Opriessnig, Gimenez-Lirola & Halbur 2011. O diagnóstico baseia-se no isolamento do agente a partir de lesões suspeitas e, principalmente, em testes sorológicos para estabelecimento de medidas de controle (Coelho et al. 2004, Xie et al. 2013.A pleuropneumonia suína (PPS) causada pela bactéria Actinobacillus pleuropneumoniae (App), provoca doença clí-nica caracterizada por pneumonia com pleurisia fibrinosa, lesões pulmonares necrohemorrágicas, adesões pleurais fibrinóticas e, em casos severos, à morte (Bossé et al. 2002). O contágio geralmente ocorre após inalação de aerossóis ou contato direto, o microrganismo inalado coloniza o tecido pulmonar aderindo-se ao muco, proteínas e a células hospedeiras com posterior multiplicação no local (Chiers et al. 2010). O monitoramento do status da doença em rebanhos, o controle e a erradicação são de fundamental importância devido a características de contágio rápido deste agente, para impedir que animais portadores sejam introduzidos em rebanhos saudáveis (Tremblay et al. 2013).Testes indiretos são muito utilizados (Machado et al. 2001, Shin et al. 2011, Eamens et al. 2012a,b, GimenezLirola et al. 2014,para detecção simultânea de anticorpos para toxinas com sensibilidade de 82,7% e especificidade de 100% (Gimenez-Lirola et al. 2014). São descritos testes de ELISA monovalentes e polivalentes para sorotipos, com sensibilidade e especificidade variando de 88,3% a 96,6% (Machado et al. 2001). A importância de alta sensibilidade e especific...

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Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection

Cited by 321 publications

References 100 publications

Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Molecular serotyping and antimicrobial resistance profiles ofActinobacillus pleuropneumoniaeisolated from pigs in South Korea

Antimicrobial susceptibility of Actinobacillus pleuropneumoniae strains isolated from pig's clinical samples in Serbia

Padronização da técnica de nanopartícula de ouro não modificada (AuNPs) para detecção de Actinobacillus pleuropneumoniae em pulmões de suínos

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