Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae

Abstract

A novel actinomycete strain, YIM 0006 T , was isolated from soil of a tropical rainforest in northern Thailand. The isolate displayed the following characteristics: aerial mycelium is absent, short spore chains are formed directly on the substrate mycelium, contains meso-diaminopimelic acid, arabinose and galactose (cell-wall chemotype IV), the diagnostic phospholipid is phosphatidylcholine, MK-9(H 4 ) is the predominant menaquinone and the G+C content of the genomic DNA is 69.0 mol%. Phylogenetic analysis and phenotypic characteristics showed that strain YIM 0006 T belongs to the family Pseudonocardiaceae but can be distinguished from representatives of all genera classified in the family. The novel genus and species Actinomycetospora chiangmaiensis gen. nov., sp. nov. are proposed, with strain YIM 0006 T (5CCTCC AA 205017 T 5DSM 45062 T ) as the type strain of Actinomycetospora chiangmaiensis.The first description of the family Pseudonocardiaceae was given by Embley et al. (1988), and the description was emended by Stackebrandt et al. (1997) et al., 1996) and Thermocrispum (Korn-Wendisch et al., 1995). Strain YIM 0006 T was isolated during an investigation of actinomycete diversity in soil from a tropical rainforest in Chiang Mai, in northern Thailand. Here, we report on the classification and characterization of strain YIM 0006 T and propose a novel genus and species of the family Pseudonocardiaceae to accommodate the strain.Strain YIM 0006 T was isolated from a soil sample after 2 weeks incubation at 28 u C on starch-glycerol medium as described by Jiang et al. (2006). Cultural characteristics of the strain were determined after growth at 28 u C for 2 weeks by methods used in the International Streptomyces Project (ISP; Shirling & Gottlieb, 1966) as well as by using Czapek's medium and nutrient agar (Dong & Cai, 2001). Colour determination was performed with colour chips from the ISCC-NBS Color Charts Standard Samples no. 2106 (Kelly, 1964). Morphological observations of spore chains and mycelia were made by light microscopy (Olympus microscope BH-2) and scanning electron microscopy (Philips XL30 ESEM-TMP) after 20-50 days incubation. Gram staining (Hucker's modification; Society for American Bacteriologists, 1957) and Ziehl-Neelsen preparations (Gordon, 1967) were evaluated by light microscopy.Growth of strain YIM 0006 T was poor on most media tested, although the strain grew well but slowly on yeast extractmalt extract agar (ISP 2). Soluble pigments were not produced under any conditions tested in this study. Aerial mycelium was not observed on any of the tested media. The vegetative mycelium fragmented into rod-shaped elements (Fig. 1a) and was pale to brilliant orange-yellow in colour. Short spore chains were formed directly on the vegetative mycelium (Fig. 1a, b). The strain displayed bud-like structures of the spore chains, as has also been described for members of the genus Pseudonocardia (Huang et al., 2002). Spores were short and rod-shaped, 0.3-0.660.8-1.2 mm. The spore surface was smooth.The...

Description of Actinomycetospora chibensis sp. nov., Actinomycetospora chlora sp. nov., Actinomycetospora cinnamomea sp. nov., Actinomycetospora corticicola sp. nov., Actinomycetospora lutea sp. nov., Actinomycetospora straminea sp. nov. and Actinomycetospora succinea sp. nov. and emended description of the genus Actinomycetospora

“…of the genus is the type species Actinomycetospora chiangmaiensis, which was originally isolated from soil of a tropical rainforest in northern Thailand (Jiang et al, 2008).…”

“…Bar, 0.01 K nuc . The description of the genus is as given by Jiang et al (2008), with the following modifications. The predominant menaquinone is MK-8(H 4 ).…”

Thermotunica guangxiensis gen. nov., sp. nov., isolated from mushroom residue compost

“…Subsequently, 14 genera have been added to family, Prauserella (Kim & Goodfellow 1999), Actinoalloteichus (Tamura et al, 2000), Crossiella (Labeda, 2001), Actinomycetospora (Jiang et al, 2008), Allokutzneria , Goodfellowiella , Sciscionella (Tian et al, 2009), Actinophytocola (Indananda et al, 2010), Alloactinosynnema (Yuan et al, 2010), Haloechinothrix (Tang et al, 2010), Yuhushiella (Mao et al, 2011) and Labedaea (Lee, 2012). Labeda & Kroppenstedt (2000) proposed the family Actinosynnemataceae to accommodate the genera of Actinokineospora, Actinosynnema, Lentzea and Saccharothrix.…”

Large numbers of new bacterial taxa found by Yunnan Institute of Microbiology

“…Members of 3 new genera, Actinobispora [16], Actinomycetospora [17] and Planosporangium [18] were found in soil samples collected from tropical rain forest in Xishuangbanna, Yunnan. A member of a new genus, Naxibacter [19], was found in coniferous forest at an elevation of 3700 m in Lijiang, northwestern Yunnan.…”