Action mechanism of Yi Guan Jian Decoction on CCl4 induced cirrhosis in rats

Mu, Yongping; Liu, Ping; Du, Guanhua; Du, Jin-xing; Wang, Gaoqiang; Long, Ai-Hua; Wang, Lei; Li, Fenghua

doi:10.1016/j.jep.2008.09.032

Cited by 36 publications

(35 citation statements)

References 24 publications

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“…3) Hepatic fibrosis is thought to be a reversible disease; however, there is no satisfactory method in clinical practice to reverse the pathological process. 17) In recent years, researchers [18][19][20] have found many traditional chinese drugs had a very good therapeutic effects in preventing fibrogenesis and other causes of chronic liver injury, which helps to develop a more hopeful future in controlling liver fibrosis injury and fibrosis. PHL is a mainly active monomer isolated from Malus hupehensis.…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective Effects of Phloridzin on Hepatic Fibrosis Induced by Carbon Tetrachloride against Oxidative Stress-Triggered Damage and Fibrosis in Rats

Deng

Wang

Zhang

et al. 2012

Biological & Pharmaceutical Bulletin

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Section: Discussionmentioning

confidence: 99%

Hepatoprotective Effects of Phloridzin on Hepatic Fibrosis Induced by Carbon Tetrachloride against Oxidative Stress-Triggered Damage and Fibrosis in Rats

Deng

Wang

Zhang

et al. 2012

Biological & Pharmaceutical Bulletin

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“…Hepatic Hyp content was examined using the previously reported method with slight modifications [19] . Briefly, 100 mg of liver tissue was homogenized and dissolved in 5 mL of 6 mol/L HCL at 110 °C for 18 h. The hydrolysate was filtered through a Millipore filter (0.45 μm, Millipore, Darmstadt, Germany), and 100 μL was removed to a clean tube and dried at 40 °C.…”

Section: Histological Examinationsmentioning

confidence: 99%

“…Gelatin zymography MMP2 and MMP9 activity were assessed using gelatin zymography as previously described [19,21] . Total protein was extracted with the addition of RIPA solution (containing 1 mol/L Tris-HCl and 0.5 mol/L NaCl, pH 7.0) and separated using electrophoresis in 0.1% gelatin and an 8% stacking gel at 4 °C, followed by elution with elution buffer (containing 2.5% Triton X-100, 50 mmol/L Tris-HCl, 5 mmol/L CaCl 2 , and 1 mmol/L ZnCl 2 , pH 7.6) for 45 min, twice.…”

Section: Western Blot Analysismentioning

confidence: 99%

Chinese medicine CGA formula ameliorates DMN-induced liver fibrosis in rats via inhibiting MMP2/9, TIMP1/2 and the TGF-β/Smad signaling pathways

Peng

Sun

et al. 2016

Acta Pharmacol Sin

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Aim: Chinese medicine CGA formula consists of polysaccharide from Cordyceps sinensis mycelia (CS-PS), gypenosides and amygdalin, which is derived from Fuzheng Huayu (FZHY) capsule for treating liver fibrosis. In this study we attempted to confirm the therapeutic effects of CGA formula in dimethylnitrosamine (DMN)-induced liver fibrosis in rats, and to identify the mechanisms of anti-fibrotic actions. Methods: Rats were injected with DMN (10 mg·kg -1 ·d -1, ip) for 3 consecutive days per week over a 4-week period. The rats then were orally administered with CGA formula (CS-PS 60 mg·kg -1 ·d -1, gypenosides 50 mg·kg -1 ·d -1 and amygdalin 80 mg·kg -1 ·d -1 ) daily in the next 2 weeks. CS-PS, gypenosides or amygdalin alone were administered as individual component controls, whereas colchicine and FZHY were used as positive controls. Serum biomarkers were measured. Hepatic injury, collagen deposition and stellate cell activation were examined. The MMP activities, expression of TIMP protein and proteins involved in the TGF-β1/Smad signaling pathways in liver tissues were assayed. Results: In DMN-treated rats, administration of CGA formula significantly decreased serum ALT, AST and total bilirubin and hepatic hydroxyproline levels, increased serum albumin level, and attenuated liver fibrosis as shown by histological examination. Furthermore, these effects were comparable to those caused by administration of FZHY, and superior to those caused by administration of colchicine or the individual components of CGA formula. Moreover, administration of CGA formula significantly decreased the protein levels of α-SMA, TGF-β1, TGF-β1 receptor (TβR-I), p-TβR-I, p-TβR-II, p-Smad2, p-Smad3, TIMP1 and TIMP2, as well as MMP2 and MMP9 activities in liver tissues of DMN-treated rats. Conclusion: Chinese medicine CGA formula ameliorates DMN-induced liver fibrosis in rats, and this effect was likely associated with the down-regulation of MMP2/9 activities, TIMP1/2 protein expression and the TGF-β1/Smad signaling pathways in the liver.

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“…In the CC14 rat model, Yiguanjian could significantly inhibit the expression of Fas, Bax, Caspase-12, Caspase-3 protein, reduce hepatocyte apoptosis index, suggesting that Yiguanjian might play protecting role to liver cells and anti-fibrosis by inhibiting liver cells apoptosis [36][37][38]. The tests in vitro showed that Yiguanjian also could induce apoptosis of hepatic stellate cells and anoikis of Bel-7402 hepatoma cells [39,40], showing Yiguanjian's positive regulation to different cells.…”

Section: Regulating Apoptosismentioning

confidence: 95%

Researching Prospect of Correspondence between Prescription and Syndromes on the Pathologic Basis of Yin Deficiency Syndrome of Liver and Kidney and Effective Mechanism of Decoction of Yiguan Jian

Wl¹,

Ww²,

Xl³

et al. 2017

J Dev Drugs

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The theory of correspondence between prescription and syndrome is an important starting point for studying modern TCM. Yin deficiency syndrome of liver and kidney is a common clinical syndrome found in many chronic diseases, and modern researches had found its pathobiological basis involving synthetic and metabolic disorders of the body material, chronic inflammation, and damage and apoptosis of cells, etc. Decoction of Yiguan Jian is an effective prescription for Yin deficiency syndrome of liver and kidney, commonly used in clinical treatment of a variety of chronic diseases. Based on summarizing studies on pathobiological basis of Yin deficiency syndrome of liver and kidney and clinical effects of Decoction of Yiguan Jian, this article has discussed the meaning of correspondence between prescription and syndrome in the treatment of Yin deficiency syndrome of liver and kidney with decoction of Yiguan Jian, demonstrated the practical values and developing prospects, and provided new ideas for the study of correlation of syndrome and treatment.

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Action mechanism of Yi Guan Jian Decoction on CCl4 induced cirrhosis in rats

Cited by 36 publications

References 24 publications

Hepatoprotective Effects of Phloridzin on Hepatic Fibrosis Induced by Carbon Tetrachloride against Oxidative Stress-Triggered Damage and Fibrosis in Rats

Hepatoprotective Effects of Phloridzin on Hepatic Fibrosis Induced by Carbon Tetrachloride against Oxidative Stress-Triggered Damage and Fibrosis in Rats

Chinese medicine CGA formula ameliorates DMN-induced liver fibrosis in rats via inhibiting MMP2/9, TIMP1/2 and the TGF-β/Smad signaling pathways

Researching Prospect of Correspondence between Prescription and Syndromes on the Pathologic Basis of Yin Deficiency Syndrome of Liver and Kidney and Effective Mechanism of Decoction of Yiguan Jian

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