Activated peripheral blood mononuclear cell mediators trigger astrocyte reactivity

Bellaver, Bruna; Rocha, Andréia Silva da; Souza, Débora Guerini de; Leffa, Douglas Teixeira; Bastiani, Marco Antônio De; Schu, Guilherme; Ferreira, Pamela C.L.; Venturin, Gianina Teribele; Greggio, Samuel; Ribeiro, Camila Tiefensee; Costa, Jaderson Costa da; Moreira, José Cláudio Fonseca; Gelain, Daniel Pens; Torres, Iraci Lucena da Silva; Klamt, Fábio; Zimmer, Eduardo R.

doi:10.1016/j.bbi.2019.05.041

Cited by 15 publications

(10 citation statements)

References 53 publications

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“…In this study, the decrease of GFAP expression in the model group was initially unexpected. However, it has been established that astrocytes are activated during inflammation [35]. Nevertheless, evidence of reduced GFAP immunoreactivity following traumatic brain injury (TBI) has been reported previously and is attributed to the breakdown of intermediate filaments and a concomitant alteration in overall protein function [36].…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Zhang

Xie

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objectives. To study the influence of electroacupuncture (EA) and moxibustion on the hippocampus astrocyte and microglia activation in the ulcerative colitis model and to evaluate the mitochondria activity. Methods. 2.5% dextran sodium sulfate-induced colitis mice were treated by EA or moxibustion. Intestinal pathological structure was observed by hematoxylin and eosin (H&E) staining; the expression of GFAP or S100b (markers for astrocyte), Iba-1 (a marker for microglia), and Mitofilin (a marker for mitochondria) in hippocampus was detected by immunofluorescence staining or western blot. Results. The results demonstrated that both EA and moxibustion could improve the morphology of distal colonic mucosal epithelia in DSS-induced colitis mice. Expression of GFAP in the hippocampus was significantly increased after EA or moxibustion treatment. The effects were further supported by WB results. Meanwhile, expression of mitofilin in the hippocampus CA1 and CA3 regions showed the same trend as that of GFAP. Expression of Iba-1 in the hippocampus showed no significant difference after EA or moxibustion treatment, while the state of microglia changed from resting in control mice to activated state in colitis mice. Conclusion. EA and moxibustion were able to modulate the activation of astrocyte, microglial, and mitochondria in the hippocampus area in the colitis model.

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Section: Discussionmentioning

confidence: 99%

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Zhang

Xie

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Moreover, neonatal HI increases the assortativity coefficient, indicating a possible compensatory mechanism through a core of jointly interconnected nodes, in an attempt to increase MBN resilience (Rubinov & Sporns, 2010). Research using different experimental models such as epilepsy (Zanirati et al., 2018), sepsis (Bellaver et al., 2019), as well as clinical research on adult‐onset neurodegenerative diseases (Horwitz et al., 1987; Huang et al., 2018) reported a reduction in MBN connection as an indicator of metabolic brain architecture disruption.…”

Section: Discussionmentioning

confidence: 99%

“…Data normality distribution was tested using the Kolmogorov-Smirnov test, which showed a non-parametric profile for the variables: glucose me- sults was conducted and no data was excluded from the analysis. The number of animals required for each experiment was estimated based on previous experience of authors and data available in the literature using a similar methodology (Bellaver et al, 2019;Couto-Pereira et al, 2019;Odorcyk et al, 2020). Thereby, it was estimated that 10-14 animals of each group would be adequate for behavioral assessment and 6-10 animals for the other parameters (metabolism, biochemical, and morphological studies), in order to achieve the power of 80% and significance of p< .05.…”

Section: Discussionmentioning

confidence: 99%

“…In short, global efficiency is a measure of how effectively the network interchange information between its nodes; density quantifies the overall number of edges of a network; assortativity coefficient measures the network resilience; average degree refers to the number of connections a node of a graph has with other nodes, and the clustering coefficient corresponds to the portion of the node's neighbors that are also neighbors of each other (Rubinov & Sporns, 2010). MBNs were corrected for multiple comparisons using false discovery rate (FDR) at p < .001 (Bellaver et al., 2019; Zanirati et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

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Pre‐ and early postnatal enriched environmental experiences prevent neonatal hypoxia‐ischemia late neurodegeneration via metabolic and neuroplastic mechanisms

Durán‐Carabali

Odorcyk

Greggio

et al. 2020

Journal of Neurochemistry

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Prenatal and early postnatal periods are important for brain development and neural function. Neonatal insults such as hypoxia‐ischemia (HI) causes prolonged neural and metabolic dysregulation, affecting central nervous system maturation. There is evidence that brain hypometabolism could increase the risk of adult‐onset neurodegenerative diseases. However, the impact of non‐pharmacologic strategies to attenuate HI‐induced brain glucose dysfunction is still underexplored. This study investigated the long‐term effects of early environmental enrichment in metabolic, cell, and functional responses after neonatal HI. Thereby, male Wistar rats were divided according to surgical procedure, sham, and HI (performed at postnatal day 3), and the allocation to standard (SC) or enriched condition (EC) during gestation and lactation periods. In‐vivo cerebral metabolism was assessed by means of [18F]‐FDG micro‐positron emission tomography, and cognitive, biochemical, and histological analyses were performed in adulthood. Our findings reveal that HI causes a reduction in glucose metabolism and glucose transporter levels as well as hyposynchronicity in metabolic brain networks. However, EC during prenatal or early postnatal period attenuated these metabolic disturbances. A positive correlation was observed between [18F]‐FDG values and volume ratios in adulthood, indicating that preserved tissue by EC is metabolically active. EC promotes better cognitive scores, as well as down‐regulation of amyloid precursor protein in the parietal cortex and hippocampus of HI animals. Furthermore, growth‐associated protein 43 was up‐regulated in the cortex of EC animals. Altogether, results presented support that EC during gestation and lactation period can reduce HI‐induced impairments that may contribute to functional decline and progressive late neurodegeneration.

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“…The SUV was calculated for the whole brain and each individual region. In order to correct for weight variations, we calculated the SUV ratio (SUVr) of each individual brain region by dividing the SUV value of the region by the whole brain SUV [57][58][59]. Mean SUVs of 14 brain regions were extracted using a prede ned VOI template.…”

Section: Chronic Early Treatment With Methylphenidatementioning

confidence: 99%

Evidence that methylphenidate Treatment Evokes Anxiety-Like Behavior Through Glucose Hypometabolism and Disruption of the Orbitofrontal Cortex Metabolic Networks

Schmitz

Silveira

Venturin

et al. 2021

Preprint

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Methylphenidate (MPH) has been widely misused by children and adolescents who do not meet all diagnostic criteria for attention-deficit/hyperactivity disorder without a consensus about the consequences. Here, we evaluate the effect of MPH treatment on glucose metabolism and metabolic network in the rat brain, as well as on performance in behavioral tests. Wistar male rats received intraperitoneal injections of MPH (2.0 mg/kg) or an equivalent volume of 0.9% saline solution (controls), once a day, from the 15th to the 44th day of age. Fluorodeoxyglucose-18 was used to investigate the cerebral metabolism, and a cross-correlation matrix was used to examine the brain metabolic network in MPH-treated rats using micro-positron emission tomography imaging. Performance in the light-dark transition box, eating-related depression, and sucrose preference tests were also evaluated. While MPH provoked glucose hypermetabolism in the auditory, parietal, retrosplenial, somatosensory, and visual cortices; hypometabolism was identified in the left orbitofrontal cortex. MPH-treated rats show a brain metabolic network more efficient and connected, but careful analyzes reveal that the MPH interrupts the connection of the orbitofrontal cortex with other brain areas. Anxiety-like behavior was also observed in MPH-treated rats. This study shows that glucose metabolism evaluated by microPET in the brain can be affected by MPH in different ways according to the region of the brain studied. It may be related, at least in part, to a rewiring in brain the metabolic network and behavioral changes observed, representing an important step in exploring the mechanisms and consequences of MPH treatment.

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Activated peripheral blood mononuclear cell mediators trigger astrocyte reactivity

Cited by 15 publications

References 53 publications

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Pre‐ and early postnatal enriched environmental experiences prevent neonatal hypoxia‐ischemia late neurodegeneration via metabolic and neuroplastic mechanisms

Evidence that methylphenidate Treatment Evokes Anxiety-Like Behavior Through Glucose Hypometabolism and Disruption of the Orbitofrontal Cortex Metabolic Networks

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