Activation of complement by DMSO and ethanol and its inhibition by soluble complement receptor type 1

Laver, Alison; Hibbs, Martin; Smith, Richard A.

doi:10.1042/bst023167s

“…Several investigations indicate that DMSO may cause activation of the complement cascade in human serum that could contribute to DMSOrelated side effects. 7,[12][13][14] Therefore, the development of a DMSO-free method of cryopreservation might improve the safety of hematopoietic cell transplantation by both, by reducing the side effects for the patient and by avoiding toxic effects on the cryoprotected cells.…”

Section: Poietic Stem Cellsmentioning

confidence: 99%

Adoption of long-term cultures to evaluate the cryoprotective potential of trehalose for freezing hematopoietic stem cells

Scheinkönig

¹

,

Kappicht

²

,

Hj

³

et al. 2004

Bone Marrow Transplant

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Summary:Dimethylsulfoxide (DMSO), which is widely used as a cryoprotectant for hematopoietic stem cells (HSC), has considerable toxicity for both the thawed cells and the patient. The aim of this study was to evaluate the cryoprotective potential of trehalose in comparison to DMSO for human HSC. Human bone marrow (BM) and peripheral blood stem cells (PBSC) of volunteer donors were cryopreserved in the presence of different concentrations of trehalose with and without insulin, as well as with DMSO 10%. After thawing to 371C colony-forming unit (CFU) assays were performed. Long-term marrowcultures (LTMC) were established and used for the detection of long-term culture-initiating cells (LTCIC). The total amount of CFUs detected was 1047134 (mean7s.d.) in DMSO-preserved cells and 179734 in trehalose-protected cells. For LTMC the best feeder layer proved to be fresh human BM and the most useful concentration of trehalose was 0.5 M. Using these culture conditions we could detect after 5 weeks LTMC a total of 172728 CFUs for trehalose-protected cells and 170752 for DMSO-preserved cells. In conclusion, trehalose exerts a similar cryoprotective potential for hematopoietic progenitor and stem cells like DMSO and could possibly replace DMSO at least in part as cryoprotectant in the setting of hematopoietic cell transplantation.

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“…These microscopic lesions in mesangial cells [33], other glomerular structures [20], and the basal micro-invaginations [21] of proximal tubular cells, as well as the postulated loss of microenvironment integrity of integral membrane proteins and their function [29], are likely to determine the quality and quantity of kidney function and the restoration of energy resources in transplants. The importance of these findings should be evaluated in light of new immunological findings [9,10,12,19,20,22]. Comparing our histological observations with the 31P analyses, we found a better correlation of phospholipid indicators to glomerular struc- ture (GPC/PME-to-hdi ratio: r = 0.9543 and P = 0.0031) and to glomerular function (GPC/PME to K+-diuresis ratio: r = 0.8275 and P = 0.0421), whereas the comparable TN/PME-to-hdi ratio was r = 0.1002 and P = 0.8503, and the TN/PME-to-K+-diuresis ratio was r = 0.0737 and P = 0.8900.…”

Section: Discussionmentioning

confidence: 99%

“…Hypothermic storage is known to lead to defects in cells, even at temperatures of 8 "C-25 "C, in the absence of cryoprotective agents [17,181. At these temperatures, the toxicity of protective agents (e. g., butylated hydroxytoluene, DMSO) is so great [19,38] that the net benefit is limited. Storage at subzero temperatures is much more useful because the ischemic metabolism is retarded and the resealing process of phospholipid bilayers is highly accelerated by DMSO [18].…”

Section: Discussionmentioning

confidence: 99%

Nonfreezing cryopreservation – a possible means of improving long-term transplant function?

Banafsche

¹

,

Pomer

²

,

Staehler

³

1998

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Improving organ preservation techniques for transplantation is one of the most important goals of transplantation research. We have established a new, nonfreezing cryopreservation method to optimize the viability of rat kidneys for transplantation with up to 4 M dimethylsulphoxide (DMSO) in EuroCollins solution (EC) at -5 degrees C to -15 degrees C. We have confirmed the occurrence of a tubular and glomerular defect pattern that mediates acute tubular necrosis (ATN) and that may be a cause of major histocompatibility complex (MHC) independent immunological components of chronic transplant rejection. The extent of this defect [transplant survival and function, 31P-NMR spectroscopy, histological defect index] in the nonfreezing cryopreserved groups (n = 22) is significantly (P = 0.017) lower than in the simple cold storage group (n = 12). Quality and localization of the lesions in kidney transplants can elucidate the context of organ preservation, progressive hyperfiltration defects, and the occurrence of graft failure without elevated frequency of acute rejection episodes. These results indicate that further efforts to provide higher pretransplant organ viability without using it to prolong cold storage intervals may provide better insight into MHC-independent factors of chronic transplant failure and may result in improved long-term transplant outcome.

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Nonfreezing cryopreservation - a possible means of improving long-term transplant function?

Banafsche

¹

,

Pomer

²

,

Staehler

³

1998

Transplant International

1

0

View full text Add to dashboard Cite

Improving organ preservation techniques for transplantation is one of the most important goals of transplantation research. We have established a new, nonfreezing cryopreservation method to optimize the viability of rat kidneys for transplantation with up to 4 M dimethylsulphoxide (DMSO) in EuroCollins solution (EC) at -5 degrees C to -15 degrees C. We have confirmed the occurrence of a tubular and glomerular defect pattern that mediates acute tubular necrosis (ATN) and that may be a cause of major histocompatibility complex (MHC) independent immunological components of chronic transplant rejection. The extent of this defect [transplant survival and function, 31P-NMR spectroscopy, histological defect index] in the nonfreezing cryopreserved groups (n = 22) is significantly (P = 0.017) lower than in the simple cold storage group (n = 12). Quality and localization of the lesions in kidney transplants can elucidate the context of organ preservation, progressive hyperfiltration defects, and the occurrence of graft failure without elevated frequency of acute rejection episodes. These results indicate that further efforts to provide higher pretransplant organ viability without using it to prolong cold storage intervals may provide better insight into MHC-independent factors of chronic transplant failure and may result in improved long-term transplant outcome.

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Activation of complement by DMSO and ethanol and its inhibition by soluble complement receptor type 1

Cited by 5 publications

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Adoption of long-term cultures to evaluate the cryoprotective potential of trehalose for freezing hematopoietic stem cells

Adoption of long-term cultures to evaluate the cryoprotective potential of trehalose for freezing hematopoietic stem cells

Nonfreezing cryopreservation – a possible means of improving long-term transplant function?

Nonfreezing cryopreservation - a possible means of improving long-term transplant function?

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