2017
DOI: 10.1038/s41598-017-16049-2
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Activation of endogenous arginine vasopressin neurons inhibit food intake: by using a novel transgenic rat line with DREADDs system

Abstract: Various studies contributed to discover novel mechanisms of central arginine vasopressin (AVP) system responsible for the behaviour albeit endogenous vasopressin activation. We established a novel transgenic rat line which expresses both human muscarinic acetylcholine receptors (hM3Dq), of which ligand is clozapine-N-oxide (CNO), and mCherry fluorescence specifically in AVP neurons. The mCherry neurons that indicate the expression of the hM3Dq gene were observed in the suprachiasmatic (SCN), supraoptic (SON), … Show more

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Cited by 43 publications
(45 citation statements)
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“…The role of magnocellular vasopressin cells is less well explored, although they are also active during feeding 110 and appear to have an anorectic action. 188,189 However, vasopressin is not only expressed in magnocellular neurones, but also in parvocellular neurones of the paraventricular nucleus that regulate the stress axis, in neurones of the suprachiasmatic nucleus that regulate circadian rhythms, 190 and in diverse other populations, including in the olfactory bulbs 191 and retina. 192 Accordingly, the behavioural roles of vasopressin might reflect compartmentalisation of function in different subsets of neurones.…”
mentioning
confidence: 99%
“…The role of magnocellular vasopressin cells is less well explored, although they are also active during feeding 110 and appear to have an anorectic action. 188,189 However, vasopressin is not only expressed in magnocellular neurones, but also in parvocellular neurones of the paraventricular nucleus that regulate the stress axis, in neurones of the suprachiasmatic nucleus that regulate circadian rhythms, 190 and in diverse other populations, including in the olfactory bulbs 191 and retina. 192 Accordingly, the behavioural roles of vasopressin might reflect compartmentalisation of function in different subsets of neurones.…”
mentioning
confidence: 99%
“…Tissue specimens were prepared and stained followed by analysis via florescence microscopy, as described previously 26,53 . The sections for the L4, SON and PVN were mounted on glass slides.…”
Section: Methodsmentioning
confidence: 99%
“…The sections for L4 were determined based on Figure 116 of the Rat Brain in Stereotaxic Coordinates atlas by Paxinos and Watson (32), and the images were obtained using a digital camera (DS-L2, DS-Fi1; Nikon Corp.). Fos-immunoreactive (ir) cells in lamina I and II of both ipsilateral and contralateral L4 spinal dorsal horns were counted manually with each captured image, as described previously (31,(33)(34)(35)(36). Three sections of L4 were counted for each animal, and the results were averaged for each group at each evaluation time (n = 5-6 per group and evaluation time).…”
Section: Fos Labeling On Spinal Cord Sectionsmentioning
confidence: 99%