Activation of melanoblasts and melanocytes after treatment with monochromatic excimer light and narrowband‐ultraviolet B of skin of vitiligo patients

Hirobe, Tomohisa; Enami, Hisao

doi:10.1111/ijd.14216

Cited by 7 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…UVB-based phototherapy has been clinically proven to be efficient for inducing repigmentation (5,10). different skin repigmentation patterns, such as perifollicular, marginal, diffuse and combined patterns, have been observed in vitiligo patients who have undergone UVB-based phototherapy, suggesting that the replenishment of melanocytes probably comes from diverse sources in the epidermis and/or in hair follicles (11). Over the past decades, effort has been devoted to investigating whether dormant melanocyte stem cells that reside in the bulge region of hair follicles are awakened to participate in the perifollicular repigmentation of the skin (5,(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Role of the p53‑TRPM1/miR‑211‑MMP9 axis in UVB‑induced human melanocyte migration and its potential in repigmentation

Miao

Jiang

et al. 2020

Int J Mol Med

View full text Add to dashboard Cite

clinical studies have proven that ultraviolet B (UVB) based phototherapy can induce perifollicular and marginal repigmentation patterns in the skin of vitiligo patients. It is, however, difficult to conceive how melanocytes can easily exit from their tightly interconnected epidermal microenvironment to re-enter a different location in the skin to establish a new network with neighboring keratinocytes. While it is known that matrix metalloprotease 9 (MMP9) is involved in the degradation of the extracellular matrix in physiological or pathological processes, little is known about whether MMP9 affects melanocyte migration in vitiligo repigmentation. To investigate the effects of the p53-transient receptor potential cation channel subfamily M member 1 (TRPM1)/microRNA (miR/miRNA)-211-MMP9 axis to regulate melanocyte migration following exposure to UVB, the expression profile of MMP9 in cultured human melanocytes transfected with or without the miR-211-mimic and p53-GFP lentiviral vector, respectively were determined. Quantitative polymerase chain reaction and western blotting were used to examine p53, TRPM1 and MMP9 mRNA and protein levels in UVB-exposed and unexposed cells. The capacity of melanocytes to migrate on collagen IV substrate was estimated using a Transwell migration assay. Interestingly, the upregulation of p53 and MMP9 at the mRNA and protein levels was evident in melanocytes treated with single or repeat exposures to UVB, whereas levels of TRPM1 and miR-211 were significantly suppressed in UVB-exposed melanocytes compared with the UVB-unexposed control cells. These results indicate that the p53-TRPM1/miR-211-MMP9 axis is significantly activated in melanocytes exposed to UVB. Notably, the ability of melanocyte migration was altered by the overexpression of p53 using a lentiviral vector and by the upregulation of miR-211 using an miRNA mimic. That altered migration could be neutralized by co-treatment with GM6001 (a broad-spectrum MMP inhibitor). Overall, these results show that the MMP9-mediated migration of melanocytes is regulated by a novel mechanism driven by the p53-TRPM1/miR-211-MMP9 axis. Activation of the p53-TRPM1/miR-211-MMP9 axis potentially represents an attractive therapeutic target to improve repigmentation outcomes in vitiligo patients.

show abstract

Section: Introductionmentioning

confidence: 99%

Role of the p53‑TRPM1/miR‑211‑MMP9 axis in UVB‑induced human melanocyte migration and its potential in repigmentation

Miao

Jiang

et al. 2020

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…In our study, 38 normal skin samples were derived from 28 patients, because four patients donated multiple body sites (Table 1, number 3 to 5, number 6 and 7, number 8 to 12 and number 14 to 17). Three punch biopsies (0.6–1.3 mm in diameter, 1.5 mm in depth) per each skin site including arms, legs, scalp, back, belly, cheek, forehead, ears, hands, neck, eyelids, chin, buttock, shoulder and penis were obtained using electric micro drill at 90 g (SIRIUS NT MICRO, Gerlach Sirius Nt Micro, Eduard Gerlach GmbH, Luebeck, Germany) as reported previously [13–16].…”

Section: Methodsmentioning

confidence: 99%

The human melanocyte and melanoblast populations per unit area of epidermis in the rete ridge are greater than in the inter‐rete ridge

Hirobe¹,

Enami²,

Nakayama³

2021

Intern J of Cosmetic Sci

Self Cite

View full text Add to dashboard Cite

Objective In human skin, melanocytes and melanoblasts are mostly located in the epidermis in addition to hair follicles. In hairy skin such as mouse skin, the inter‐follicular epidermis is generally flat. In contrast, in human skin, the epidermis is wavy and possesses well‐developed rete ridges. It is not well understood what difference exists in cell function between melanocytes present in the inter‐rete ridge and those in the rete ridge. To clarify this problem, we first tried to determine the melanocyte and melanoblast populations per unit area of epidermis both in the rete ridge and inter‐rete ridge epidermis. Methods Thirty‐eight samples from normal skin sites of 28 patients (14 males and 14 females) aged from 5 to 76 years were fixed and processed to dopa and combined dopa‐premelanin reactions. The numbers of cells positive to the dopa (melanocytes) and combined dopa‐premelanin (melanoblasts and melanocytes) reactions were scored. Results The average melanocyte population/0.1 mm2 of the inter‐rete ridge was 74 cells, whereas that of the rete ridge was 147 cells. Moreover, the average melanoblast population/0.1 mm2 in the inter‐rete ridge was 43 cells, whereas that of the rete ridge was 131 cells. The melanoblast population in the rete ridge differed between female and male, but the melanocyte populations in the rete ridge and inter‐rete ridge and the melanoblast population in the inter‐rete ridge did not differ between female and male. However, no age difference in the melanocyte and melanoblast populations was observed between the rete ridge and inter‐rete ridge. Conclusion Human epidermal melanocytes and melanoblasts in the rete ridge exist more numerously than in the inter‐rete ridge.

show abstract

“…Three punch biopsies per each skin site were collected. The methods for obtaining punch biopsies were reported previously 3 …”

Section: Patient Gender Age Skin Collagen Elastinmentioning

confidence: 99%

“…The skin samples were fixed with 16% formalin in phosphate buffer (pH 7.0) for 16–24 hours at 2°C. The samples were then washed with distilled water and incubated with a 0.1% L‐3,4‐dihydroxyphenylalanine (dopa, Wako Pure Chemical Industry, Osaka, Japan) solution in phosphate buffer (pH 7.4) for 16–24 hours at 37°C as reported previously 3 . The dopa reaction reveals only tyrosinase‐containing differentiated melanocytes.…”

Section: Patient Gender Age Skin Collagen Elastinmentioning

confidence: 99%

See 1 more Smart Citation

Elastin fiber but not collagen fiber is decreased dramatically in the dermis of vitiligo patients

Hirobe¹,

Enami²,

Nakayama³

2020

Int J Dermatology

Self Cite

View full text Add to dashboard Cite

Activation of melanoblasts and melanocytes after treatment with monochromatic excimer light and narrowband‐ultraviolet B of skin of vitiligo patients

Abstract: These results suggest that exposures with MEL/NB-UVB induce the marginal or perifollicular repigmentation pattern in the white macules, though the two patterns produce no differences in the distribution and density of melanoblasts/melanocytes in the lesional epidermis.

Cited by 7 publications

References 33 publications

Role of the p53‑TRPM1/miR‑211‑MMP9 axis in UVB‑induced human melanocyte migration and its potential in repigmentation

Role of the p53‑TRPM1/miR‑211‑MMP9 axis in UVB‑induced human melanocyte migration and its potential in repigmentation

The human melanocyte and melanoblast populations per unit area of epidermis in the rete ridge are greater than in the inter‐rete ridge

Elastin fiber but not collagen fiber is decreased dramatically in the dermis of vitiligo patients

Contact Info

Product

Resources

About