Activation of mouse peritoneal macrophages by mannophosphoinositides of mycobacteria

Abstract

Peritoneal macrophages isolated from mannoside-methylated bovine serum albumin (MBSA)-immunized mice showed significantly enhanced phagocytosis of Mycobacterium smegmatis compared to control or MBSA-immunized groups. Immune macrophages also exhibited bacteriostatic activity against M. smegmatis. Pretreatment of mycobacteria with mannoside antibodies did not further alter the phagocytosis and bacteriostatic effect of immune macrophages.

Immunoprotective behaviour of liposome entrapped cell wall subunit of Mycobacterium tuberculosis against experimental tuberculous infection in mice

“…Phagocytic activity of peritoneal macrophages was investigated as described previously [21], with some modifications. Briefly, peritoneal macrophages isolated from control and immunized mice were cultured in RPMI-1640 containing 10% fetal calf serum (FCS), for 24 h at 37°C in an atmosphere of 5% C0 2 • These macrophages were then used as effector cells, and incubated with labelled M. smegmatis cells (target cells) in a ratio of 1:10 for 2 h. To examine the phagocytosis of M. smegmatis cells, macropbages were lysed with lysing solution (0.25% sodium dodecy1 sulphate) and radioactivity in lysate was counted.…”