Activation of Rice nicotianamine synthase 2 (OsNAS2) Enhances Iron Availability for Biofortification

Lee, Sichul; Kim, You-Sun; Jeon, Un Sil; Kim, Eui Chong; Schjøerring, Jan K.; An, Gynheung

doi:10.1007/s10059-012-2231-3

Cited by 123 publications

(86 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NA is also involved in long-distance iron transport in the phloem and translocation to the grains (Puig et al 2007). Constitutive expression of OsNAS2 also increased iron content in polished rice (Johnson et al 2011; Lee et al 2012). Moreover, mugineic acid (MA) and NA function in the uptake of other essential micronutrients, including zinc, copper, and manganese (Haydon and Cobbett 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Masuda et al (2009) showed that constitutive overexpression of the HvNAS1 gene in rice resulted in a threefold increase of iron in the rice endosperm. Similarly, constitutive overexpression of OsNAS2 increased iron content in polished rice by 2- to fourfold (Johnson et al 2011; Lee et al 2012). In addition to iron, the zinc content was also increased in polished grains of NAS overexpressing lines (Johnson et al 2011; Wirth et al 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rice NICOTIANAMINE SYNTHASE 2 expression improves dietary iron and zinc levels in wheat

et al. 2016

View full text Add to dashboard Cite

Key message Iron and zinc deficiencies negatively impact human health worldwide. We developed wheat lines that meet or exceed recommended dietary target levels for iron and zinc in the grains. These lines represent useful germplasm for breeding new wheat varieties that can reduce iron and zinc deficiency-associated health burdens in the affected populations. AbstractMicronutrient deficiencies, including iron and zinc deficiencies, have negative impacts on human health globally. Iron-deficiency; anemia affects nearly two billion people worldwide and is the cause of reduced cognitive development, fatigue and overall low productivity. Similarly, zinc deficiency causes stunted growth, decreased immunity and increased risk of respiratory infections. Biofortification of staple crops is a sustainable and effective approach to reduce the burden of health problems associated with micronutrient deficiencies. Here, we developed wheat lines expressing rice NICOTIANAMINE SYNTHASE 2 (OsNAS2) and bean FERRITIN (PvFERRITIN) as single genes as well as in combination. NAS catalyzes the biosynthesis of nicotianamine (NA), which is a precursor of the iron chelator deoxymugeneic acid (DMA) required for long distance iron translocation. FERRITIN is important for iron storage in plants because it can store up to 4500 iron ions. We obtained significant increases of iron and zinc content in wheat grains of plants expressing either OsNAS2 or PvFERRTIN, or both genes. In particular, wheat lines expressing OsNAS2 greatly surpass the HarvestPlus recommended target level of 30 % dietary estimated average requirement (EAR) for iron, and 40 % of EAR for zinc, with lines containing 93.1 µg/g of iron and 140.6 µg/g of zinc in the grains. These wheat lines with dietary significant levels of iron and zinc represent useful germplasm for breeding new wheat varieties that can reduce micronutrient deficiencies in affected populations.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-016-2808-x) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rice NICOTIANAMINE SYNTHASE 2 expression improves dietary iron and zinc levels in wheat

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Nicotianamine-Zn complexes and yellow-stripe-like transport proteins also influence grain Zn concentration by facilitating root Zn uptake, xylem loading, translocation to shoots, storage in shoots and phloem transport (Curie et al 2009;Lee et al 2012;Zhou et al 2013). Anchoring genetic map to physical map in the future studies can ascertain a possible role of identified QTL in regulating formation of nicotianamine-Zn complexes and yellow-stripe-like transport proteins.…”

Section: Identified Qtl and Physiology Of Zinc Mobilitymentioning

confidence: 96%

Mapping QTL associated with remobilization of zinc from vegetative tissues into grains of barley (Hordeum vulgare)

et al. 2015

View full text Add to dashboard Cite

Background and aims Limited remobilization of Zn from vegetative tissues into grains via phloem is a major physiological barrier against Zn loading into cereal grains. In present experiment, doubled-haploid mapping population (150 lines, derived from Clipper ×Sahara) of barley was genetically characterized for differential Zn remobilization. Methods The germplasm was grown under glasshouse conditions. Leaves (upper three), stem (the rest of the plant) and mature grains were sampled from the maintillers at anthesis and maturity for Zn analysis.Quantitative trait loci (QTL) regulating time to anthesis, plant biomass, Zn concentration in vegetative tissues and remobilization of Zn from these tissues into grains were identified using a genetic linkage map of 485 markers. Results A significant variation existed in grain Zn concentration among the lines (27-75 μg Zn g −1 ), and it correlated with the amount of Zn remobilized from vegetative tissues into grains. Sahara remobilized 37 % of pre-anthesis Zn reserves into grains; the presence of its alleles at all QTL associated with leaf (3 QTL) and stem (2 QTL) Zn remobilization increased the trait score.Conclusions Present study provided an insight into the genetic basis of Zn remobilization from vegetative tissues into barley grains. Such information is useful in breeding for Zn biofortification.

show abstract

“…These studies found up to 2.6-fold more Fe and 2.7-fold more Zn, in polished grain when the genes were activated, individually, in transgenic rice. Furthermore, seed from both OsNAS2 and OsNAS3 activation tagged lines reversed signs of Fe-deficiency when fed to anemic mice, indicating strong potential for NAS overexpression lines to improve iron stores in Fe-deficient humans (Lee et al 2009(Lee et al , 2012. Johnson et al (2011) recently described constitutive overexpression of all three of the rice NAS genes, individually, using the full length CaMV 35S promoter in cv.…”

Section: Enhancing the Chelation-based Fe Uptake System Of Rice -Nutrmentioning

confidence: 98%

Enhancing the chelation capacity of rice to maximise iron and zinc concentrations under elevated atmospheric carbon dioxide

Johnson¹

2013

Functional Plant Biol.

View full text Add to dashboard Cite

Roughly half of the Earth's seven billion people rely on rice as their primary source of food. The milled grain of rice, often referred to as polished or white rice, serves as a rich source of energy but is low in protein and several essential micronutrients such as iron and zinc. As a result, billions of people in rice-based countries suffer the debilitating effects of protein-energy and micronutrient malnutrition with symptoms including iron-deficiency anaemia, growth retardation and blindness. By 2050, the Earth's atmospheric carbon dioxide concentration ([CO 2 ]) is expected to reach 550 mmol mol -1 , representing a 70% increase from today's concentration of 392 mmol mol -1 . The impacts of elevated [CO 2 ] on plant growth will likely include agronomically useful traits such as increased biomass, yield and water-use efficiency. However, increased plant productivity is likely to be accompanied by decreased protein and micronutrient mineral concentrations of cereal grain. This review focuses on the effects of carbon dioxide-enrichment on rice physiology and nutritional composition and proposes increased activity of the Strategy II iron uptake pathway as a promising method to maintain or increase iron and zinc concentrations in rice grain, and perhaps cereal grain in general, under elevated [CO 2 ].

show abstract

Activation of Rice nicotianamine synthase 2 (OsNAS2) Enhances Iron Availability for Biofortification

Cited by 123 publications

References 33 publications

Rice NICOTIANAMINE SYNTHASE 2 expression improves dietary iron and zinc levels in wheat

Rice NICOTIANAMINE SYNTHASE 2 expression improves dietary iron and zinc levels in wheat

Mapping QTL associated with remobilization of zinc from vegetative tissues into grains of barley (Hordeum vulgare)

Enhancing the chelation capacity of rice to maximise iron and zinc concentrations under elevated atmospheric carbon dioxide

Contact Info

Product

Resources

About