Activities of antioxidant system components and polyphenol oxidase in ontogeny of lawn grasses under megapolis conditions

Половникова, Марина Григорьевна; Voskresenskaya, O. L.

doi:10.1134/s1021443708050154

Cited by 13 publications

(6 citation statements)

References 12 publications

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“…The action of polyphenol oxidase, one of the terminal oxidases in the plant cell, was improved under unfavorable conditions. This enzyme, alongside peroxidase, engaged in the oxidation of phenolic components, which are cancer prevention agents that favor cell resistance [26]. Therefore, a measurement of the concentration of oxidizing phenols could be representative of cell's vulnerability.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Assessment of Antioxidant Enzymes, Phenolic Contents and Antioxidant Capacity of the Verdolaga (Portulacaceae)

Almulaiky

Aldhahri

Al‐Abbasi

et al. 2020

IJN

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In this study, the antioxidants and photosynthetic compounds of Verdolaga were examined. Compounds were extracted from distinctive segments of the verdolaga using various solvents such as methanol (40, 60, 80%), ethanol (40, 60, 80%), acetone (40, 60, 80%), and deionized water. The use of 80% methanol led to the highest extracted concentration of phenolic substances and flavonoids. The extracted products (Leaves, Stem strips, and Root strips) were evaluated for their radical scavenging capabilities with DPPH (IC50= 22.26, 20.56, and 32.10), and ABTS (IC50= 2.86, 3.70, and 5.24), reducing power (EC50= 15.70, 16.39, and 21.69), and peroxide scavenging activity (1C50= 1.717, 2.937, and 3.255), respectively. The extracted products were analyzed by a gas chromatography-mass spectrometer. Peroxidase, catalase, and polyphenol oxidase assays were completed for the crude extract of verdolaga’s leave, stem strips, and root strips. As indicated by these tests, extracts of the verdolaga’s roots, stems and leaves using 80% methanol yielded high antioxidant activity. The most elevated concentrations of extracted chlorophyll, lycopene, and carotenoids were from the leaves and the highest concentration of extracted tannin was noted from strips of stems. The highest measures of peroxidase and polyphenol oxidase were identified in root strips and the highest units of catalase was identified in leaves.

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Section: Resultsmentioning

confidence: 99%

In Vitro Assessment of Antioxidant Enzymes, Phenolic Contents and Antioxidant Capacity of the Verdolaga (Portulacaceae)

Almulaiky

Aldhahri

Al‐Abbasi

et al. 2020

IJN

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“…However, PPO activity is affected to a greater extent by (generally unknown) external factors, as indicated in several studies: (i) in unpublished results of Van Ranst et al the average PPO activity measured in 14 red clover cultivars after re-growth in the greenhouse was found to be about 2-fold higher compared with the PPO activity measured in the same red clover cultivars grown in the field. Furthermore, the effect of the environment (greenhouse v. field) differed between cultivars; (ii) Winters et al (2008) found an effect of growing season as a 4-fold difference in PPO activity in red clover leaves that was measured between June and July; (iii) Fothergill and Rees (2006) also measured a seasonal effect, but in addition, they found a 5-fold higher activity in September 2005 compared with September 2004 and a 2-fold lower activity in May 2005 compared with May 2006; (iv) PPO activity was shown to be up to 5-fold higher when red clover was grown in a polluted city area compared with a relatively unpolluted area (Polovnikova and Voskresenskaya, 2008). An effect of growth stage (Polovnikova and Voskresenskaya, 2008) and re-growth duration (Lee et al, 2009a) was also reported.…”

Section: Ppo Activity In Red Clovermentioning

confidence: 94%

“…Furthermore, the effect of the environment (greenhouse v. field) differed between cultivars; (ii) Winters et al (2008) found an effect of growing season as a 4-fold difference in PPO activity in red clover leaves that was measured between June and July; (iii) Fothergill and Rees (2006) also measured a seasonal effect, but in addition, they found a 5-fold higher activity in September 2005 compared with September 2004 and a 2-fold lower activity in May 2005 compared with May 2006; (iv) PPO activity was shown to be up to 5-fold higher when red clover was grown in a polluted city area compared with a relatively unpolluted area (Polovnikova and Voskresenskaya, 2008). An effect of growth stage (Polovnikova and Voskresenskaya, 2008) and re-growth duration (Lee et al, 2009a) was also reported. Nevertheless, Lee et al (2004) selected red clover plants (cv.…”

Section: Ppo Activity In Red Clovermentioning

confidence: 94%

Red clover polyphenol oxidase and lipid metabolism

Ranst

Lee

Fievez

2011

Animal

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Increasing the polyunsaturated fatty acid (PUFA) composition of milk is acknowledged to be of benefit to consumer health. Despite the high PUFA content of forages, milk fat contains only about 3% of PUFA and only about 0.5% of n-3 fatty acids. This is mainly due to intensive lipid metabolism in the rumen (lipolysis and biohydrogenation) and during conservation (lipolysis and oxidation) such as drying (hay) and ensiling (silage). In red clover, polyphenol oxidase (PPO) has been suggested to protect lipids against degradation, both in the silage as well as in the rumen, leading to a higher output of PUFA in ruminant products (meat and milk). PPO mediates the oxidation of phenols and diphenols to quinones, which will readily react with nucleophilic binding sites. Such binding sites can be found on proteins, resulting in the formation of protein-bound phenols. This review summarizes the different methods that have been used to assess PPO activity in red clover, and an overview on the current understanding of PPO activity and activation in red clover. Knowledge on these aspects is of major importance to fully harness PPO's lipid-protecting role. Furthermore, we review the studies that evidence PPO-mediated lipid protection and discuss its possible importance in lab-scale silages and further in an in vitro rumen system. It is demonstrated that high (induction of) PPO activity can lead to lower lipolysis in the silage and lower biohydrogenation in the rumen. There are three hypotheses on its working mechanism: (i) protein-bound phenols could directly bind to enzymes (e.g. lipases) as such inhibiting them; (ii) binding of quinones in and between proteins embedded in a lipid membrane (e.g. in the chloroplast) could lead to encapsulation of the lipids; (iii) direct binding of quinones to nucleophilic sites in polar lipids also could lead to protection. There is no exclusive evidence on which mechanism is most important, although there are strong indications that only lipid encapsulation in protein-phenol complexes would lead to an effective protection of lipids against ruminal biohydrogenation. From several studies it has also become apparent that the degree of PPO activation could influence the mode and degree of protection. In conclusion, this review demonstrates that protein-bound phenols and encapsulation in protein-phenol complexes, induced by PPO-mediated diphenol oxidation, could be of interest when aiming to protect lipids against pre-ruminal and ruminal degradation.Keywords: red clover, polyphenol oxidase, lipid metabolism, rumen, silage ImplicationsPolyphenol oxidase (PPO) is an enzyme that has been found to be active in red clover. There is a whole range of recent studies exploring possibilities of using this enzyme to protect membrane lipids against degradation in the forage during storage and in the rumen of ruminants. Protection of lipids against degradation is of importance as it could help to improve the fatty acid profile of ruminant products, with respect to consumer health. This review aims to summarize...

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“…At the high concentration of anthropogenic pollutants in the environment (industrial zone) polyphenoloxidase activity in leaves of red clover (sensitive plant to pollution) rose substantially (by 2-3 times in comparison with its roots). 48 It is oen that the activity of glutathione-S-transferase is induced by heavy metals. 21 As such, it was expected that induced glutathione-S-transferase activities would be found in the present study.…”

Section: Resultsmentioning

confidence: 99%

Investigation of antioxidant and detoxifying capacities of some date cultivars (Phoenix dactylifera L.) irrigated with sewage water

et al. 2017

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Activities of antioxidant system components and polyphenol oxidase in ontogeny of lawn grasses under megapolis conditions

Cited by 13 publications

References 12 publications

In Vitro Assessment of Antioxidant Enzymes, Phenolic Contents and Antioxidant Capacity of the Verdolaga (Portulacaceae)

In Vitro Assessment of Antioxidant Enzymes, Phenolic Contents and Antioxidant Capacity of the Verdolaga (Portulacaceae)

Red clover polyphenol oxidase and lipid metabolism

Investigation of antioxidant and detoxifying capacities of some date cultivars (Phoenix dactylifera L.) irrigated with sewage water

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