Activity and distribution of carbonic anhydrase in leaf and ear parts of wheat (Triticum aestivumL.)

Li, Xingjun; Hou, Jihua; Bai, K. V. K.; Yang, Xinghong; Lin, Jinxing; Li, Zhensheng; Kuang, Tingyun

doi:10.1016/j.plantsci.2003.10.031

Cited by 20 publications

(13 citation statements)

References 38 publications

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“…Recent investigations showed that CA activity of wheat leaves increased to the maximum at the flag-leaf visible stage, and then decreased to a minimum before tending to increase at the dough stage, whereas CA activity in both lemmas and pericarps considerably increased from the milk to the dough stage. CA activities at the milk stage were significantly higher in ear parts than in leaf tissues (Li et al 2004). …”

Section: Resultsmentioning

confidence: 96%

Effect of Water Deficit on Rubisco and Carbonic Anhydrase Activities in Different Wheat Genotypes

Guliyev

Bayramov

Babayev

2008

Photosynthesis. Energy From the Sun

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An influence of drought stress on the dynamics of activities of ribulose 1.5-bisphosphate carboxylase/oxygenase (Rubisco) and carbonic anhydrase (CA) in bread and durum wheat genotypes differing in their drought tolerance and productivity was investigated under field conditions. Rubisco activity was primarily constant in plants with normal watering and subjected to drought, while it decreased at the duration of vegetation in all genotypes. CA activity increased during the first period of drought in flag leaves of drought resistance genotypes, but decreased at the last stage of vegetation. The activities of Rubisco and CA were lower in ear elements at an early stage in generative development than in flag leaves and decreased less in flag leaves under water stress.

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Section: Resultsmentioning

confidence: 96%

Effect of Water Deficit on Rubisco and Carbonic Anhydrase Activities in Different Wheat Genotypes

Guliyev

Bayramov

Babayev

2008

Photosynthesis. Energy From the Sun

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“…They have an important function in grain formation and grouting protein accumulation (Davood andAlireza 2011, Zhang et al 2012). Carbonic anhydrase (CA) possibly functions in C 4 photosynthesis at the dough stage in wheat plants (Li et al 2004). Studies on NAD-malic enzyme (NAD-ME), NADP-malic enzyme (NADP-ME), NADP-malate dehydrogenase (MDH), and pyruvate Pi dikinase (PPDK) are focused on the physiology and protein expression in wheat seedling leaves Bassham 1983, 1984a,b;Gajewska et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Response of wheat ear photosynthesis and photosynthate carbon distribution to water deficit

Jia¹,

Lv²,

Jiang³

et al. 2015

Photosynt.

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Understanding distribution and transport of carbon assimilates and photosynthesis contribution to grain yield in wheat spike is important in assessing the photosynthetic process under stress conditions. In this study, photosynthetic characteristics were evaluated in a pot experiment. Transport of spike photosynthates to grain was demonstrated using 14 C isotope tracer technique. Yield and key enzyme activities of C 3 and C 4 pathways were examined after anthesis in wheat cultivars of different drought resistance. The ear net photosynthetic rate, chlorophyll content of the spike bracts (glume, lemma, and palea), and relative water content slightly decreased under water deficit in drought resistant variety Pubing 143 (Pub) during the grain filling stage, whereas all parameters decreased significantly in drought sensitive variety Zhengyin 1 (Zhe). Grain 14 C-photosynthate distribution rate fell by 3.8% in Pub and increased by 3.9% in Zhe. After harvest, the water-use efficiency of Zhe dropped by 18.7% under water deficit. Rubisco activity in ear organs declined significantly under water deficit, whereas activity of C 4 pathway enzymes was significantly enhanced, especially that of phosphoenolpyruvate carboxylase and NADP-malate dehydrogenase. Water deficit exerted lesser influence on spike photosynthesis in Pub. Ear organs exhibited delayed senescence. Accumulation of photosynthetic carbon assimilates in ear bracts occurred mainly during the early grain filling and photosynthates were transported in the middle of grain filling. C 4 pathway enzymes seem to play an important function in ear photosynthesis. We speculate that the high enzyme activity of the C 4 pathway and the increased capacity of photosynthetic carbon assimilate transport were the reasons for the drought tolerance characteristics of ears. Additional key words:14 C-labelling; harvest index; malic enzyme; spike assimilate transport; Triticum aestivum L.

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“…Rubisco activase is a nuclear-encoded chloroplastic protein, which enables Rubisco to function under varying physiological conditions (Crafts-Brandner and Salvucci 2000). Carbonic anhydrase provides adequate levels of inorganic carbon to accelerate carbon assimilation through carboxylases such as Rubisco (Li et al 2004). Carbonic anhydrase constitutes 1-2% of the total soluble proteins in leaves of C 3 plants, second only to Rubisco in concentration and was prominently observed as five different spots in this study.…”

Section: Discussionmentioning

confidence: 99%

Proteomic approach to study leaf proteins in a fast-growing tree species, Gmelina arborea Linn. Roxb

Rasineni

Chinnaboina

Reddy

2009

Trees

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Foliar proteome studies have become highly significant for a comprehensive understanding of complex processes associated with plant growth and development.In the present study, we present a proteomic approach to analyze leaf proteins in an important timber-yielding and fast-growing forest tree species, Gmelina arborea Linn. Roxb. (Verbanaceae). Foliar protein analysis involved protein extraction, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF-TOF). From the 2-DE protein profile of Gmelina leaves, we identified and isolated 150 well-separated protein spots; among these, 64 protein spots were identified by mass spectrometric (MS/MS) analysis. These proteins were classified according to their involvement in basic biological functions, such as photosynthesis, amino acid metabolism, cytoskeleton, cell wall metabolism, stress-related proteins, redox maintenance, electron transport chain, phytohormone metabolism and protein translation and folding. Analytical variance was determined for the protein spots of samples from different plants. The present study is believed to provide a foundation for the use of leaf proteomics in addressing fundamental physiological and biochemical processes associated with growth and productivity of tree species such as Gmelina arborea.

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Activity and distribution of carbonic anhydrase in leaf and ear parts of wheat (Triticum aestivumL.)

Cited by 20 publications

References 38 publications

Effect of Water Deficit on Rubisco and Carbonic Anhydrase Activities in Different Wheat Genotypes

Effect of Water Deficit on Rubisco and Carbonic Anhydrase Activities in Different Wheat Genotypes

Response of wheat ear photosynthesis and photosynthate carbon distribution to water deficit

Proteomic approach to study leaf proteins in a fast-growing tree species, Gmelina arborea Linn. Roxb

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