The effects of feeding different levels of protein and vitamin B ]2 on egg production and hatchability of eggs of Single Comb White Leghorn hens were studied. Diets containing 16 and 32% corn-soybean meal protein with and without 10 |xg. of vitamin B 12 per kg. of diet were employed. Egg production and fertility of eggs were not significantly affected by protein level or vitamin B T2 supplement. However, increasing protein level in the hen diets resulted in a significant decrease in hatchability. Vitamin B, 2 supplementation of both diets significantly improved hatchability of eggs. However, the supplementary vitamin B , 2 was not adequate to support desirable hatchability when the diet contained 32% protein.The high level of protein, and the addition of vitamin B l2 to the 16% protein diet significantly increased egg weight.Chicks hatched from eggs produced by hens fed the different experimental diets were used to study the effect of breeder diets on chick growth and livability. A deficiency of vitamin B , 2 in the breeder had a detrimental effect on chick growth and livability. Increasing the protein level of the chick diet from 22 to 32% resulted in a further decrease in growth when the chicks were derived from vitamin B, 2 deficient hens. A high level of protein in the breeder diet also depressed chick growth. This growth depression was not counteracted by a dietary supplement of 500 p.g. of vitamin B, 2 /kg., by an oral dose of 50 |xg. of vitamin B 12 /chick at hatching, or by an injection of 1 or 10 jj,g. of vitamin B, 2 / bird at hatching. Vitamin B 12 supplied through the hen diet was more effective than supplementation of chick diet in stimulating early chick growth. Mortality was higher in chicks hatched from hens fed the 16% protein diet than in those from hens fed 32% protein. This was the reverse of embryo mortality during incubation.