Activity of Glutathione Antioxidant System and NADPH-Generating Enzymes in Rats with Experimental Rheumatoid Arthritis

Крыльский, Е. Д.; Попова, Т. Н.; Kirilova, E. M.

doi:10.1007/s10517-015-3089-0

Cited by 15 publications

(5 citation statements)

References 7 publications

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“…On the 15th day after the resulting inflammation in the absence of treatment, the specific activity of all antioxidant defense enzymes (SOD, catalase, GR, G6PD, LDR directed and LDR reversed) increased, which characterizes their activation as a response to oxidative stress developing against the background of RA. These results are consistent with the studies of the authors, which demonstrated a significant increase in enzyme activity in various tissues (liver, heart, muscles, blood serum), from 1.2 to 1.7 times [48][49][50][51][52].…”

Section: Biological Activity Of the Fa Patch On The Adjuvant-induced ...supporting

confidence: 93%

Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model

Konnova,

Volkov,

Solovyeva

et al. 2023

Sci. Pharm.

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The formulation of the transdermal patch with fulvic acid (FA) on an emulsion basis using pluronic Kolliphor® p237 as a permeability enhancer was developed and studied for anti-inflammatory properties. FA was isolated from the peat in the Nizhny Novgorod region of Russia and characterized as a potential active pharmaceutical ingredient. In vitro studies of the release of FA from the transdermal patch, as well as the FA penetration through the acetyl cellulose membrane using the Franz diffusion cell, showed its high efficiency (56% and 90%, respectively, in 8 h). In the in vivo experiment, qualitative and quantitative features of the rat knee caused by complete Freund’s adjuvant-induced arthritis (morphological changes, the FA influence on the biochemical indexes) were studied. The inflammatory process that developed within 15 days was accompanied by the activation of antioxidant oxidoreductase enzymes (by 50–70%), the increase in the cross-sectional diameter of the cartilage, and the increase in the values of marker indicators of the process of rheumatoid arthritis. Within 7 days of treatment, under the influence of FA, the values of ESR, RF, leukocytes, C-reactive protein, as well as the biochemical parameters characterizing oxidative stress (SOD, catalase, glutathione reductase, LDH, G6PD) normalized, and the edema reduced. These results may be useful for arthritis treatment using the transdermal patch with FA.

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Section: Biological Activity Of the Fa Patch On The Adjuvant-induced ...supporting

confidence: 93%

Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model

Konnova,

Volkov,

Solovyeva

et al. 2023

Sci. Pharm.

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“…Apocynin is a well-known NADPH-oxidase inhibitor, and there have been several studies on the therapeutic effect of apocynin in chronic inflammatory diseases such as atherosclerosis [7], rheumatoid arthritis [10], airway inflammation [9], stroke and IBD [12,13]. Many in vitro and in vivo experiments have shown the therapeutic potential of apocynin as an anti-inflammatory agent.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of apocynin on dextran sulfate sodium-induced mouse colitis model

et al. 2019

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Background and aim Various drugs have been developed for inflammatory bowel disease (IBD), but still there are limitations in the treatment due to the insufficient responses and significant adverse effects of immunosuppressant. Apocynin is an NADPH-oxidase inhibitor with established safety profiles. We aimed to investigate the protective efficacy of apocynin in IBD using chemical-induced mouse colitis model. Method We induced experimental colitis by administrating 5% dextran sulfate sodium (DSS) to 8-week old BALB/c mouse for 11 days. Apocynin (400 mg/kg) or sulfasalazine (150 mg/kg) were administeredduring7 days. We monitored bodyweight daily and harvested colon and spleen at day 11 to check weight and length. We also examined histopathologic change and pro-, anti-inflammatory cytokines and enzymes from harvested colons (iNOS, COX-2, TNF-α, MCP-1, p-NrF2, and HO-1). Result Apocynin significantly alleviated weight reduction induced by DSS treatment (21.64 ± 0.55 for Apocynin group vs. 20.33 ± 0.90 for DSS group, p = 0.005). Anti-inflammatory efficacy of apocynin was also shown by the recovery of colon weight and length. Histopathologic examination revealed significantly reduced inflammatory foci and erosions by apocynin treatment. Colonic expression of iNOS, COX-2, TNF-α, and MCP-1 was decreased significantly in the apocynin treated group. Anti-inflammatory mediators Nrf2 and HO-1 were activated significantly in apocynin treated mouse. Conclusion Apocynin showed significant anti-inflammatory efficacy against chemically induced colonic inflammation. This study also revealed the unique action of apocynin compared to the currently prescribed drug, sulfasalazine. Given its excellent safety profile and potent efficacy with novel action mechanism, apocynin can be a new therapeutic molecule for the IBD treatment, which can be added to the currently available drugs.

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“…The activity of the antioxidant enzymes, such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GP), and glutathione reductase (GR), was evaluated in hemolyzed erythrocytes. The content of SOD was determined by calculating the speed of auto-oxidation of quercetin in the presence of tetramethylethylenediamine [ 40 ]; the activity of catalase was measured by the reaction with ammonium molybdate [ 41 ]; the activity of GP was deduced from the reaction between sulfhydryl groups of reduced glutathione and Elman's reagent [ 42 ]; and the GR-activity was assessed by the NADPH-dependent conversion of oxidized glutathione to its reduced form [ 43 ]. The total protein content was determined by the Bradford microassay [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

The Cardio- and Neuroprotective Effects of Corvitin and 2-Oxoglutarate in Rats with Pituitrin-Isoproterenol-Induced Myocardial Damage

Tkachenko

Kovalchuk

Бондаренко

et al. 2018

Biochemistry Research International

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Heart diseases, especially acute coronary syndrome (ACS), are among the most severe illnesses that often lead to death. Despite significant advances in the prevention and treatment of ACS, the incidence of the disease and its complications are very serious. The imbalance between pro- and antioxidant systems, the formation of active carbonyl compounds, and the end products of glycation in the blood and tissues are the key moments in the development of heart and neurological disorders leading to a change of behavioral responses. So, the search for antioxidants with cardio- and neuroprotective effects is an urgent task. This study was aimed at evaluating the effects of Corvitin and 2-oxoglutarate on physiological parameters, heart histology, and markers of carbonyl/oxidative stress of rats with pituitrin-isoproterenol-induced myocardial damage (PIMD). Increased sweating, tachycardia, significantly decreased locomotor and exploratory activity, changes of ECG, heart histology, and biochemical changes were observed in the PIMD-group. The administration of Corvitin or 2-OG led to the recovery of locomotor and cognitive activities of the rats, improvement in heart histology, a decrease in the levels of thiobarbituric acid reactive substances, advanced glycated end products, and various changes in the activity of the antioxidant enzymes, 6 days after PIMD. So, Corvitin and exogenous 2-OG show cardio- and neuroprotective effects through the decrease of carbonyl/oxidative stress and regulation of the activity of the antioxidant system.

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Activity of Glutathione Antioxidant System and NADPH-Generating Enzymes in Rats with Experimental Rheumatoid Arthritis

Cited by 15 publications

References 7 publications

Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model

Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model

Anti-inflammatory effects of apocynin on dextran sulfate sodium-induced mouse colitis model

The Cardio- and Neuroprotective Effects of Corvitin and 2-Oxoglutarate in Rats with Pituitrin-Isoproterenol-Induced Myocardial Damage

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