Actuation of Tunable Elastomeric Pores: Resistance Measurements and Finite Element Modelling

Willmott, Geoff R.; Chaturvedi, R.C.; Cummins, Struan John Wright; Groenewegen, L G

doi:10.1007/s11340-013-9795-5

Cited by 20 publications

(40 citation statements)

References 54 publications

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“…Separation between pegs on opposite legs was varied between 45 and 49 mm but is the same across both pairs of legs, so that the strain applied to the septum is predominantly radial. 31 Aqueous electrolyte is contained within upper and lower fluid cells which surround the membrane, and Ag=AgCl electrodes are embedded in each half of the cell.…”

Section: Apparatus and Experimentsmentioning

confidence: 99%

“…35 Closer comparison of fitted and experimental parameters would need to incorporate uncertainty in two further values, the electrolyte resistivity (q ¼ 0.75 X m) which is assumed homogeneous, and the membrane thickness (d ¼ 159 lm), which is obtained using a hyperelastic material model of pore actuation. 31 Fits to multiple peaks were generated by aligning simulated events with each experimental peak, and matching the baseline current with the local experimental background. There is a good agreement with experimental data in Fig.…”

Section: Simulationsmentioning

confidence: 99%

“…The sophistication of resistive pulse sensing has also increased, making use of channels made from carbon nanotubes, 17,18 glass, [19][20][21][22][23] silicon, 5,8,15,24 polymers, [25][26][27][28] and elastomers. [29][30][31][32][33][34][35][36][37][38] Resistive pulses can now be used for study and measurement of particle size, 2,3,17,18,20,21,34 concentration, 33 and charge. 17,38 There is clear potential to extend applications towards sensing of complicated dynamic interactions 24 and specific particle shapes.…”

mentioning

confidence: 99%

“…29,30 This membrane can be reversibly actuated on macroscopic scales in order to stretch and relax the micro-to nanoscale conical pore geometry. 31 The ionic current passing through a TP can vary by at least an order of magnitude when the pore is stretched. 32 Therefore, any individual pore specimen has considerable versatility relating to the size of particles it can analyze.…”

mentioning

confidence: 99%

“…In the case of terminal blockages, replacement pores from the same fabrication batch have similar physical characteristics. 31 SPMs are finding many applications on sub-cellular length scales, especially in biomedical, 39 microfluidic, and microTAS technologies. 40 They are of particular benefit in assay development and biological handling applications, providing an efficient and cost-effective way to separate and pre-concentrate analytes from solutions.…”

mentioning

confidence: 99%

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Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

Willmott

Platt

2012

Biomicrofluidics

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Tunable pores (TPs) have been used for resistive pulse sensing of 1 lm superparamagnetic beads, both dispersed and within a magnetic field. Upon application of this field, magnetic supraparticle structures (SPSs) were observed. Onset of aggregation was most effectively indicated by an increase in the mean event magnitude, with data collected using an automated thresholding method. Simulations enabled discrimination between resistive pulses caused by dimers and individual particles. Distinct but time-correlated peaks were often observed, suggesting that SPSs became separated in pressure-driven flow focused at the pore constriction. The distinct properties of magnetophoretic and pressure-driven transport mechanisms can explain variations in the event rate when particles move through an asymmetric pore in either direction, with or without a magnetic field applied. Use of TPs for resistive pulse sensing holds potential for efficient, versatile analysis and measurement of nano-and microparticles, while magnetic beads and particle aggregation play important roles in many prospective biosensing applications.

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Section: Apparatus and Experimentsmentioning

confidence: 99%

Section: Simulationsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

Willmott

Platt

2012

Biomicrofluidics

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Probing the conformational switch of I-motif DNA using tunable resistive pulse sensing

Shi

Zhou

2018

Biochimica et Biophysica Acta (BBA) - General Subjects

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Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics

Maas

Vrij

Vlist

et al. 2015

Journal of Controlled Release

242

202

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Nano-sized extracelullar vesicles (EVs) released by various cell types play important roles in a plethora of (patho)physiological processes and are increasingly recognized as biomarkers for disease. In addition, engineered EV and EV-inspired liposomes hold great potential as drug delivery systems. Major technologies developed for high-throughput analysis of individual EV include nanoparticle tracking analysis (NTA), tunable resistive pulse sensing (tRPS) and high-resolution flow cytometry (hFC). Currently, there is a need for comparative studies on the available technologies to improve standardization of vesicle analysis in diagnostic or therapeutic settings.We investigated the possibilities, limitations and comparability of NTA, tRPS and hFC for analysis of tumor cell-derived EVs and synthetic mimics (i.e. differently sized liposomes). NTA and tRPS instrument settings were identified that significantly affected the quantification of these particles. Furthermore, we detailed the differences in absolute quantification of EVs and liposomes using the three technologies. This study increases our understanding of possibilities and pitfalls of NTA, tRPS and hFC, which will benefit standardized and large-scale clinical application of (engineered) EVs and EV-mimics in the future.

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Actuation of Tunable Elastomeric Pores: Resistance Measurements and Finite Element Modelling

Cited by 20 publications

References 54 publications

Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

Resistive pulse sensing of magnetic beads and supraparticle structures using tunable pores

Probing the conformational switch of I-motif DNA using tunable resistive pulse sensing

Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics

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