Acute Aeromonas salmonicida infection in turbot (Scophthalmus maximus L.). Histopathological and immunohistochemical studies

Coscelli, G.; Bermúdez, Roberto; Losada, Ana; Faílde, L.D.; Santos, Ysabel; Quiroga, María Isabel

doi:10.1016/j.aquaculture.2014.04.002

Cited by 33 publications

(24 citation statements)

References 56 publications

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“…salmonicida mainly affects salmonid fish, causing high mortality rate in the Atlantic salmon (Salmo salar) and the rainbow trout (Oncorhynchus mykis) (Fernández-Álvareza et al, 2016). In salmonids, different clinical presentations of the disease, varying from acute to chronic as well as subclinical form, have been described (Coscelli et al, 2014). According to statistics, the mortality caused by this disease can reach a high of nearly 100%, resulting in heavy marked-economic losses (Liu, 2017).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance of aeromonas spp. isolated from the sea trout (Salmo trutta L.) in Latvia

Revina¹,

Avsejenko²,

Cīrule³

et al. 2017

Research for Rural Development

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The aim of this study was to investigate the patterns of antibiotic resistance of Aeromonas spp. bacteria isolated from the sea trout (Salmo trutta) from the state fish hatcheries of the Institute of Food Safety, Animal Health and Environment 'BIOR', Latvia. Bacteriological investigations were performed at four state fish hatcheries located in the drainage basins of the main Latvian rivers -Daugava, Venta and Gauja, during the five-year period (2012 -2016). In fish with visible clinical signs, bacteriological samples were collected from heart, liver, spleen, kidney and ulcer surfaces. Aeromonas hydrophila and Aeromonas salmonicida were isolated from sea trouts. A total of 52 individual sea trouts were examined. Resistance to amoxicillin, ampicillin, cephalexin, colistin, doxycycline, enrofloxacin, erythromycin, florfenicol, gentamycin, kanamycin, lincomycin, neomycin, oxytetracycline, spectinomycin, streptomycin, tetracycline, trimethoprim/sulfamethoxazole was tested. The results of this study suggest a multi-drug resistance pattern among the A. hydrophila isolates. All the isolates were resistant to amoxicillin (100%), ampicillin (100%), cephalexin (100%) and erythromycin (100%). The lowest level of resistance was found against florfenicol (4.55%), gentamycin (4.55%), kanamycin (4.55%), but susceptibility was recorded to enrofloxacin, neomycin and trimethoprim/sulfamethoxazole. A. salmonicida isolates were resistant to oxytetracycline (9.38%) and tetracycline (9.38%). For other antibiotics A. salmonicida isolates were susceptible.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance of aeromonas spp. isolated from the sea trout (Salmo trutta L.) in Latvia

Revina¹,

Avsejenko²,

Cīrule³

et al. 2017

Research for Rural Development

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“…Distribution of virulent and avirulent ASS strains in turbot tissues was investigated during infection following a bath challenge; although the avirulent strains colonized initial sites such as the epidermal mucus, skin and intestine, they were rarely detected in the internal tissues; the virulent ASS strains was found in the kidney and liver at 12 hr post‐challenge and was found in the muscle at very late stages of infection; the virulent strain existed in all detected tissues until death occurred on day 7 post‐challenge, suggesting that ASS must colonize and survive within the turbot tissues for an infection to result in death of fish (Farto et al., ). The distribution of ASS in turbot tissues was investigated using an immunohistochemical method, and the pathogen presented in the internal tissues such as the spleen, liver and kidney at 96 hr post‐challenge; there was also a direct relation between the development of lesions and the presence of the bacteria (Coscelli et al., ). V. anguillarum was found to proliferate rapidly in the muscle and skin after the bacteria entered the host via the skin injury; the bacteria were subsequently transported to the immune organs (spleen and liver) and then caused a systemic infection in the fish (Liu et al., ).…”

Section: Discussionmentioning

confidence: 99%

Colonization ofAeromonas salmonicidasubsp.masoucidastrains in Atlantic salmon (Salmo salarL.) during infection

Liu

Meng

et al. 2018

Aquac Res

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Aeromonas salmonicida subsp. masoucida (ASM) is classified as atypical A. salmonicida and brought huge economic damages to the local salmonid aquaculture in China. An ASM strain named AS‐C4 was used to investigate the colonization of ASM in Atlantic salmon (Salmo salar L.) by an immersion challenge with the control group (T0, no AS‐C4), group T1 (2.67 × 104 CFU/ml AS‐C4) and group T2 (2.67 × 107 CFU/ml AS‐C4). The numbers of AS‐C4 copies in different fish tissues (gill, intestine, skin, blood, muscle, spleen, liver and kidney) were determined at different time points post challenge using the quantitative real‐time PCR (qRT‐PCR). AS‐C4 were detected in the gill and intestine as early as 0 hr after the challenge both in T1 and T2 groups, suggesting that the gill and intestine were probably the portals of entry of AS‐C4 into salmon. Although AS‐C4 could not be detected in the skin until 24 hr after the challenge in T1 group, it could be detected in the skin as early as 0 hr after the challenge in T2 group, indicating that the skin may also be a portal of entry of AS‐C4 into salmon. AS‐C4 was immediately detected in the blood within 3 hr after it entered the host, suggesting that AS‐C4 successfully invaded the bloodstream of fish. After AS‐C4 colonized the host, it colonized the internal tissues, such as the spleen, liver, kidney and muscle. The results of this study will contribute to the understanding of the pathogenesis of the ASM strains and give a broader understanding of the infection route of ASM in it's host, providing more information for the development of new therapeutic strategies to protect against this pathogen in aquaculture.

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“…Aeromonas salmonicida, the causative agent of furunculosis, is a relevant fish pathogen in aquaculture, responsible for causing significant economic losses worldwide (DallaireDufresne and Tanaka, 2014; Diamanka et al, 2013;Gonzalez et al, 2004;Janda and Abbott, 2010;Noga, 2011) due to high mortality and morbidity in a variety of fish species (e.g., salmon (Ringø et al, 2004;Verner-Jeffreys et al, 2007), trout (Imbeault et al, 2006;Nikoskelainen et al, 2001), turbot (Coscelli et al, 2014;Farto et al, 2011;Lago et al, 2012), Atlantic cod (Arnesen et al, 2010), rockfish (Kim et al, 2013), seabream (Zorrilla et al, 2003) and wolffish (Grøntvedt and Espelid, 2004)). …”

Section: Introductionmentioning

confidence: 99%

Biological control of Aeromonas salmonicida infection in juvenile Senegalese sole (Solea senegalensis) with Phage AS-A

et al. 2016

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Acute Aeromonas salmonicida infection in turbot (Scophthalmus maximus L.). Histopathological and immunohistochemical studies

Cited by 33 publications

References 56 publications

Antimicrobial resistance of aeromonas spp. isolated from the sea trout (Salmo trutta L.) in Latvia

Antimicrobial resistance of aeromonas spp. isolated from the sea trout (Salmo trutta L.) in Latvia

Colonization ofAeromonas salmonicidasubsp.masoucidastrains in Atlantic salmon (Salmo salarL.) during infection

Biological control of Aeromonas salmonicida infection in juvenile Senegalese sole (Solea senegalensis) with Phage AS-A

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